Urolithin a Partially Protects against Oxidative Damage Induced for Microcistyn‐lr in C6 Cells

Abstract

Microcystin‐LR (MIC‐LR) is a toxin which the mechanism of intoxication involves oxidative stress. Urolithin A (URO‐A) is a metabolic from colonic fermentation of ellagic acid with antioxidant potential. This study aimed to evaluate the putative protective effect of URO‐A against MIC‐LR toxicity in C6 cells. C6 cells were incubated with MIC‐LR (1 and 10 μM) and/or URO‐A (3, 30, 60 and 100 μM) for 24h. MIC‐LR induced reactive species (RS) generation, depletion in total thiol (SH) groups and survival loss when compared with control group. Also, at 10 μM, MICLR induced CAT activity inhibition. URO‐A caused CAT activity inhibition and showed a trend to increase RS generation (60 and 100 μM) per se. URO‐A at 3 μM completely attenuated the RS generation and the impairment in SH groups caused by MIC‐LR. Our results demonstrated that URO‐A might offer protective effect against toxicity caused by MIC‐LR in glial cells by restoring the levels of RS and thiol groups.