Urodynamics in acetone-induced cystitis of anesthetized rats

Shimizu, Isao; Kawashima, Katsuyoshi; Hosoki, Kanoo

doi:10.1002/(sici)1520-6777(1999)18:2<115::aid-nau7>3.0.co;2-d

Cited by 16 publications

(11 citation statements)

References 25 publications

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“…The anti-inflammatory effect of NSAIDs, such as indomethacin, helps to reduce the hypersensitivity of the bladder sensory nerves (52,53), although the anti-inflammatory effect on the bladder inflammation, which was indicated by increased bladder weight and induction of TNF-α mRNA expression, was not observed in the present administration schedule. A potent opioid analgesic, morphine, may be used for severe bladder pain and referred pain in IC patients (34), while it suppresses urinary frequency through spinal and supraspinal mechanisms by acting on μ-opioid receptors (54,55). Taken together, these results for drug sensitivity suggest that the H 2 O 2 -induced cystitis model is useful as hyperactive bladder model and is suitable to screen novel drugs for cystitis-induced urinary frequency.…”

Section: Discussionmentioning

confidence: 85%

A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide

et al. 2013

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Section: Discussionmentioning

confidence: 85%

A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide

et al. 2013

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“…Thus, CYP-treated rats have been used as a bladder pain model. On the other hand, intravesical instillation of acetone, which is known to remove the glycosaminoglycan layer of the transitional uroepithelium,5 has also been used to induce chemical cystitis 6,7. However, there have been few studies of the connection between changes in micturition, nociceptive behavior and inflammation in the CYP or acetone induced cystitis models.…”

Section: Introductionmentioning

confidence: 99%

Comparison of voiding function and nociceptive behavior in two rat models of cystitis induced by cyclophosphamide or acetone

Saitoh

Yokoyama

Chancellor

et al. 2009

Neurourology and Urodynamics

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Aims-Nociceptive behavior and its relationship with bladder dysfunction were investigated in two cystitis models, which were induced by intraperitoneal (ip) injection of cyclophosphamide (CYP) or intravesical instillation of acetone, using freely moving, non-catheterized conscious rats. Methods-FemaleSprague-Dawley rats were used. Cystitis was induced by ip injection of CYP (100 and 200mg/kg) or intravesical instillation of acetone (10, 30 and 50%) via a polyethylene catheter temporarily inserted into the bladder through the urethra. Then the incidence of nociceptive behavior (immobility with decreased breathing rates) was scored. Voided urine was collected simultaneously and continuously to measure bladder capacity. The plasma extravasation in the bladder was quantified by an evans blue (EB) dye leakage technique.Results-CYP (100mg/kg, ip) induced nociceptive behavior without affecting bladder capacity or EB concentration in the bladder. A higher dose of CYP (200mg/kg, ip) decreased bladder capacity and increased EB levels as well as nociceptive behavior. In contrast, intravesical instillation of acetone (30%) decreased bladder capacity and increased EB levels, but evoked nociceptive behavior less frequently compared with CYP-treated animals. In capsaicin pretreated rats, nociceptive behavior induced by CYP or acetone was reduced; however, the overall effects of CYP or acetone on bladder capacity and bladder EB levels were unaffected.Conclusions-These results suggest that there is a difference in the induction process of nociceptive behavior and small bladder capacity after two different types of bladder irritation and that C-fiber sensitization is more directly involved in pain sensation than reduced bladder capacity.

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“…The effect of these compounds has been demonstrated at the molecular level, where the appearance of claudin-1, zonula occludens-1, (ZO-1), occludin-1, are all modified in the bladders of both CyP and PS treated of Sprague Dawley rodent models. Other chemical stripping approaches have also been utilized, for example: acetone, xylene, acidic solutions and mustard oil instillation or injection, as well as electrical stimulation, have been proved to deplete the GAG layer and thus negatively influence urothelium barrier function by increasing permeability resulting in IC symptoms in numerous animal models (25). A recent study presented evidence that mouse bladder infused with 3% acetic acid followed by repeated treatment with a synthetic form of the antiproliferative factor (APF) results in IC related symptoms.…”

Section: Induced Ic Animal Models With Effects On Gags Depletion and mentioning

confidence: 99%

Reviewing Interstitial Cystitis Models and Treatments: A Focus on the Urothelium

F¹

2017

Razavi Int J Med

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Context: Interstitial cystitis is a multifactorial chronic and debilitating disease which is commonly associated with pain localized in the bladder region, increased urinary frequency, urgency and nocturia. Due to the high prevalence of pain in the population affected more recently the condition is often referred to as interstitial cystitis / bladder pain syndrome (IC/BPS). Evidence Acquisition: Although IC presents with many different symptoms, researchers have formulated three different theories, which are not mutually exclusive, to explain IC pathology: the first is related to the alteration of the proteoglycan and protein junction composition, structure and presence in the urothelium. The second is an immune induced IC resulting from an increased number of activated mast cells in the bladder internal layers, such as detrusor muscle (DM), and mucosa/submucosa. The third type, which is closely related to the second one, is due to a sensory nerve sensitization as an effect of neurotrophic factors molecule release. Results: Previous classification has been mirrored in the development of various in vitro and in vivo disease models created to mimic IC, as well as in the available therapies used to treat the condition to date. Conclusions: This review will summarize the most recent advances in the field, related to the different causative factors contributing to the development of the condition, the in vivo models used as well as the evidence they provide in advancing our knowledge and their limitations. The focus will be on works reporting on IC in the domain related to alterations in proteoglycans and cellular junction, specifically their composition, structure and appearance in the urothelium, and also discuss present and future therapies. Conclusions: This review will summarize the most recent advances in the field, related to the different causative factors contributing to the development of the condition, the in vivo models used as well as the evidence they provide in advancing our knowledge and their limitations. The focus will be on works reporting on IC in the domain related to alterations in proteoglycans and cellular junction, specifically their composition, structure and appearance in the urothelium, and also discuss present and future therapies.

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Urodynamics in acetone-induced cystitis of anesthetized rats

Cited by 16 publications

References 25 publications

A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide

A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide

Comparison of voiding function and nociceptive behavior in two rat models of cystitis induced by cyclophosphamide or acetone

Reviewing Interstitial Cystitis Models and Treatments: A Focus on the Urothelium

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