Urine nerve growth factor (NGF) level, bladder nerve staining and symptom/problem scores in patients with interstitial cystitis

Tonyalı, Şenol; Özdemir, Deniz; Akbıyık, Filiz; Kankaya, Duygu; Baydar, Dilek Ertoy; Ergen, Ali

doi:10.17219/acem/69231

Cited by 25 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are comparable with data from the study by Steers and Tuttle (14). Our data regarding elevated NGF are consistent with the results of other studies (17)(18)(19). NGF was discovered as a secreted protein necessary for the development of sympathetic and peripheral sensory neurons (20,21).…”

Section: Discussionsupporting

confidence: 93%

“…It has been shown that inflammation caused neuroplasticity leading to increased urinary NGF level and thus IC/ BPS (18). Some authors supposed that NGF played an important role in the pathogenesis of IC/BPS (18,22). The results obtained in this study also indicated an increase in the concentration of another marker-EGF.…”

Section: Discussionsupporting

confidence: 67%

“…Many researchers consider NGF as a potential IC/BPS biomarker. It has been established that the level of NGF in the urine can be used as a biomarker for the diagnosis of IC/BPS, for the differential diagnosis of this disease and hyperactive bladder and also may serve as a prognostic factor (18,23). Reports showing that inflammation increased the expression of NGF arouse interest in NGF as an important indicator of IC/BPS inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…Reports showing that inflammation increased the expression of NGF arouse interest in NGF as an important indicator of IC/BPS inflammation. It has been shown that inflammation caused neuroplasticity leading to increased urinary NGF level and thus IC/ BPS (18). Some authors supposed that NGF played an important role in the pathogenesis of IC/BPS (18,22).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

Sholan¹

2020

Haseki

View full text Add to dashboard Cite

The aim of this study was to determine the level of nerve growth factor (NGF) and epidermal growth factor (EGF) in an animal model of interstitial cystitis/bladder pain syndrome (IC/ BPS) and identify correlations between them. Methods: IC/BPS modeling was performed on white New Zealand female rabbits divided into six groups depending on the methods of modeling. Urine and blood levels of NGF and EGF were determined by the ELISA method. The correlation between the indicators was calculated by the Pearson correlation coefficient. Results: An increase in the concentration of NGF and EGF was detected in animals of all experimental groups; a significantly high level of these factors in blood and urine was determined in a toxic model created with the introduction of urine into the submucosal layer of the bladder wall. The correlation between NGF and EGF tended to increase by 14 days. There was a strong correlation between these indicators in the urine of animals with IC/BPS created by a 70% alcohol solution (p<0.01). A strong correlation between these indicators was observed in blood of animals with IC/BPS created by the administration of protamine sulfate and in animals of toxic model (p<0.01). Conclusion: An increase in the levels of proliferation markers is likely due to chronic inflammation process and urine toxicity. The presence of a correlation between NGF and EGF and their strengthening as the disease progresses indicates an increase in the proliferation processes. Proliferation markers can be used in the diagnosis and monitoring of IC/BPS.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

Sholan¹

2020

Haseki

View full text Add to dashboard Cite

show abstract

“…Interstitial cystitis/painful bladder syndrome (IC/PBS) is a challenging chronic bladder syndrome characterized by symptoms of urinary frequency, urgency, and pelvic pain and hypersensitivity [12]. The morbidity of IC/PBS is two to five times higher in women than in men with an estimated prevalence of 2.7% to 6.5% according to recent epidemiologic studies from the National Institutes of Health [345].…”

Section: Introductionmentioning

confidence: 99%

Treatment with low-energy shock wave alleviates pain in an animal model of uroplakin 3A-induced autoimmune interstitial cystitis/painful bladder syndrome

Zhang

Peng

et al. 2019

Investig Clin Urol

View full text Add to dashboard Cite

Purpose To investigate whether treatment with low-energy shock wave (LESW) alleviates pain and bladder dysfunction in a mouse model of uroplakin 3A (UPK3A)-induced interstitial cystitis/painful bladder syndrome (IC/PBS). Materials and Methods Forty female BALB/c mice were divided into four groups (n=10/group): Sham, Sham+LESW, UPK3A, and UPK3A+LESW. At 6 weeks of age, mice were injected with an emulsion containing water and complete Freund's adjuvant with (UPK3A and UPK3A+LESW groups) or without (Sham and Sham+LESW groups) 200 µg of UPK3A. At 10 weeks, mice received a second dose of Freund's adjuvant to booster immunization. At 12 weeks, mice underwent pain assessment and a frequency volume chart (FVC) test as the pretreatment assessment. LESW treatment and pain assessment were conducted from 13 to 15 weeks. One week after the final treatment, pain assessment and the FVC were conducted again as the post-treatment assessment. Mice were euthanized and sacrificed at 17 weeks. Results The presence of tactile allodynia and bladder dysfunction was significant in the UPK3A-injected mice. LESW raised the pain threshold and improved bladder function with decreased urinary frequency and increased mean urine output. Expression and secretion of local and systemic inflammatory markers, including tumor necrosis factor-α (TNF-α) and nerve growth factor (NGF), increased after UPK3A immunization. These markers were significantly decreased after LESW treatment (p<0.05). Conclusions LESW treatment attenuated pain and bladder dysfunction in a UPK3A-induced model of IC/PBS. Local and systemic inflammation was partially controlled, with a reduced number of infiltrated inflammatory cells and reduced levels of TNF-α and NGF.

show abstract

Bioinformatics analysis of the Hub genes and key pathways of interstitial cystitis pathogenesis

Liu

Zhang

et al. 2019

Neurourology and Urodynamics

View full text Add to dashboard Cite

Aims: This study aimed to identify suitable datasets for reanalysis and then explore potential key genes and related pathways of interstitial cystitis (IC). Methods:We searched the Gene Expression Omnibus database and three expression profile datasets and included 23 lesions of IC and 9 normal tissues in the analysis. Eight urine specimens of patients with IC and five urine specimens of healthy controls were also included. Then, these datasets were reanalyzed to determine the differentially expressed genes (DEGs), which were used to perform Gene Ontology and pathway enrichment analyses. These identified candidate genes were also applied to generate a protein-protein interaction (PPI) network.Results: Forty-two common DEGs were sorted and identified from two datasets, both of which included the samples of bladder lesions. Based on their functions and signaling pathways, these 42 DEGs are mainly classified as cell-surface proteins and are involved in the immune and inflammatory responses. The PPI network included 41 nodes. In this network, we identified 11 genes as central nodes that are involved in the immune system and the inflammatory response. Furthermore, IC with Hunner's lesions shared the same DEGs with IC without Hunner's lesions. In both subgroups (IC with and without Hunner's lesions), we identified some common DEGs shared between bladder lesions and urine samples. Conclusion: Using bioinformatics, we integrated different IC-related datasets and identified potential critical genes involved in IC that may contribute to future research on IC. K E Y W O R D Sbioinformatic, differentially expressed genes, interstitial cystitis

show abstract

Urine nerve growth factor (NGF) level, bladder nerve staining and symptom/problem scores in patients with interstitial cystitis

Abstract: NGF seems to be a promising biomarker in IC/BPS. It may help clinicians in diagnoses and patient follow-up. Thus, unnecessary, expensive and invasive tests, interventions and treatments might be avoided.

Cited by 25 publications

References 24 publications

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

Treatment with low-energy shock wave alleviates pain in an animal model of uroplakin 3A-induced autoimmune interstitial cystitis/painful bladder syndrome

Bioinformatics analysis of the Hub genes and key pathways of interstitial cystitis pathogenesis

Contact Info

Product

Resources

About