Urine miRNA Signature as a Potential Non-invasive Diagnostic and Prognostic Biomarker in Cervical Cancer

Aftab, Mehreen; Poojari, Satish; Seshan, Vaishnavi; Kumar, Sachin; Agarwal, Pallavi; Tandon, Simran; Zutshi, Vijay; Das, Bina Pani

doi:10.21203/rs.3.rs-115644/v1

Cited by 7 publications

(16 citation statements)

References 51 publications

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“…Sensitivity analysis was conducted in the 8 reports of 7 included studies and two AUC reports from one study [ 74 ] were found to be outliers ( S1 Fig ). Therefore, the two reports have been dropped in the meta-analysis of AUC of studies although no significant change has been shown in the interpretation of the result.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, a study revealed, in univariate Cox proportional hazards regression analysis, that there was a significant association between high microRNA-21 expression and poor overall survival of cervical cancer patients (P <0.05). However, the study revealed insignificant association in the multivariate Cox regression analysis (P <0.05) which was analyzed within the context of many clinical characteristics, including HPV infection, age at diagnosis, parity, clinical stage, marriage age, and histopathological grade [ 74 ]. Besides, a study conducted in China showed that the 5-year survival rate of patients with highly expressed microRNA-21 was lower than those of patients with lowly expressed microRNA-21 (p<0.05) [ 109 ].…”

Section: Resultsmentioning

confidence: 99%

“…Inveterate evidence on upregulated expression of microRNA-21 in cervical cancer cells and the identification of its target signaling pathways pinpoints using microRNA-21 as a convenient diagnostic and prognostic biomarker of cervical cancer. Changes in the levels of expression of miRNAs can stably and easily be assayed in tumor tissues, plasma, serum, and urine samples [ 74 , 85 ]. As a result, microRNAs are potential cancer biomarkers.…”

Section: Discussionmentioning

confidence: 99%

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Disease progression role as well as the diagnostic and prognostic value of microRNA-21 in patients with cervical cancer: A systematic review and meta-analysis

Gebrie

2022

PLoS ONE

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Introduction Cervical cancer is the fourth commonest and the fourth leading cause of cancer death in females globally. The upregulated expression of microRNA-21 in cervical cancer has been investigated in numerous studies, yet given the inconsistency on some of the findings, a systematic review and meta-analysis is needed. Therefore, the aim of this systematic review and meta-analysis is to investigate the role in disease progression as well as the diagnostic and prognostic value of microRNA-21 in patients with cervical cancer. Methods Literature search was carried out through visiting several electronic databases including PubMed/MEDLINE/ PubMed Central, Web of Science, Embase, WorldCat, DOAJ, ScienceDirect, and Google Scholar. After extraction, data analysis was carried out using Rev-Man 5.3, STATA 15.0 and Meta-disk 1.4. I2 and meta-bias statistics assessed heterogeneity and publication bias of the included studies, respectively. The area under summary receiver operating characteristic curve and other diagnostic indexes were used to estimate diagnostic accuracy. Result A total of 53 studies were included for this systematic review and meta-analysis. This study summarized that microRNA-21 targets the expression of numerous genes that regulate their subsequent downstream signaling pathways which promote cervical carcinogenesis. The targets addressed in this study included TNF-α, CCL20, PTEN RasA1, TIMP3, PDCD-4, TPM-1, FASL, BTG-2, GAS-5, and VHL. In addition, the meta-analysis of reports from 6 eligible studies has demonstrated that the overall area under the curve (AUC) of summary receiver operating characteristic (SROC) of microRNA-21 as a diagnostic accuracy index for cervical cancer was 0.80 (95% CI: 0.75, 0.86). In addition, evidence from studies revealed that upregulated microRNA-21 led to worsening progression and poor prognosis in cervical cancer patients. Conclusion microRNA-21 is an oncogenic microRNA molecule playing a key role in the development and progression of cervical malignancy. It has good diagnostic accuracy in the diagnosis of cervical cancer. In addition, the upregulation of microRNA-21 could predict a worse outcome in terms of prognosis in cervical cancer patients.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Disease progression role as well as the diagnostic and prognostic value of microRNA-21 in patients with cervical cancer: A systematic review and meta-analysis

Gebrie

2022

PLoS ONE

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“…Além disso, com o intuito de analisar se os miRNAs presentes na urina poderiam ser usados como biomarcadores confiáveis para diagnóstico e prognóstico do câncer cervical, Aftab et al (2021) concluíram que a combinação entre miR-145-5p, miR-218-5p e miR-34a-5p presentes na urina apresentou 100% de sensibilidade e 92,8% de especificidade na distinção entre pacientes controle e pacientes com câncer. Research, Society and Development, v. 11, n. 2, e25711225776, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i2.25776…”

Section: Resultsunclassified

O uso de microRNAs como biomarcadores do câncer cervical

Silva

2022

RSD

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Esse trabalho pretendeu descrever o papel dos miRNAs na regulação gênica do câncer cervical. Para esta revisão integrativa de literatura, utilizou-se artigos encontrados nas bases de dados PubMed, SciELO, Lilacs e Sciencedirect, selecionando trabalhos publicados entre 2011 e 2021, na língua portuguesa e inglesa, considerando-se os seguintes descritores: “microRNAs”, “regulação da expressão gênica”, “câncer de colo do útero” e “biomarcadores”. Encontrou-se um total de 156 trabalhos, sendo, por fim, selecionados 17 trabalhos para estudo. Estudos apontaram uma relação direta entre miRNAs e os estágios de desenvolvimento do câncer cervical. Diversos miRNAs, como miR-21 e miR-135a, têm sua expressão aumentada em células de câncer cervical quando comparada a seus níveis em indivíduos normais. Outros miRNAs, como miRNA-20a, miR-1246, miR-2392, miR-3147, miR-3162-5p e miR-4484, foram identificados como promissores biomarcadores para detecção de metástase em pacientes com neoplasia cervical. Já variação nos níveis de expressão de miRNAs pode interferir na sensibilidade à quimioterapia, como o miR-155 que aumenta a quimiossensibilidade à cisplatina, agente antineoplásico. Dessa forma, a análise das assinaturas de miRNAs em pacientes como câncer cervical surge como promissora ferramenta para o seu diagnóstico, prognóstico e tratamento, sendo necessária a associação desses aos métodos usuais para um melhor manejo clínico.

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“…Circulating miRNAs have the potential to serve as diagnostic markers of tumor progression and this has been the focus of an increasing number of studies [14,15]. For example, serum exosomal miR-1247-3p levels in patients with primary hepatic cancer are associated with the extent of lung metastasis [16].…”

Section: Introductionmentioning

confidence: 99%

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

Xun

Wang

et al. 2021

Preprint

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Background: Colorectal cancer is one of the most common malignancy in the world. It has been reported that cancer stem cells (CSCs) serve as the primary drivers of tumorigenesis and tumor progression. There is an urgent need to explore novel molecules that regulate CSCs or their signatures. Increasing evidence has shown that miRNAs are involved in tumorigenesis and progression. Here, we aim to explore the regulatory effect and mechanism of miR-3065-3p on the stemness of colorectal cancer.Methods: The expression of miR-3065-3p in colorectal cancer and the association of miR-3065-3p expression with prognosis of patients with colorectal cancer were analyzed using TCGA dataset or clinical cases. Gain of function in different models, including colorectal cancer cell lines and orthotopic xenograft or liver metastatic mouse model, were used to investigate the effects of miR-3065-3p on colorectal cancer stemness adn metastasis in vitro and in vivo. Cancer stemness was analyzed by detecting the ability of migration and invasion, NANOG, OCT4, and SOX2 expression, ALDH activity and sphere formation. In addition, the interaction of miR-3065-3p and cytokine receptor-like factor 1 (CRLF1) was analyzed theoretically and identified by the luciferase reporter assay. Moreover, the correlation between CRLF1 expression and miR-3065-3p was analyzed in colorectal cancer tissues. Finally, the effect of CRLF1 on the stemness and metastasis of colorectal cancer in vitro and in vivo was assessed.Results: In this report, we found that miR-3065-3p was overexpressed in colorectal cancer and that its high expression was associated with poor prognosis of patients with colorectal cancer. miR-3065-3p promotes the stemness and metastasis of colorectal cancer. Furthermore, CRLF1 was the downstream target of miR-3065-3p and inhibited the stemness of colorectal cancer. In addition, CRLF1 expression was negatively correlated with miR-3065-3p in colorectal cancer tissues. In addition, CRLF1 mediated the effects of miR-3065-3p on promoting stemness-related transcription factor expression in colorectal cancer cells.Conclusion: Our data suggest that miR-3065-3p promoted the stemness and metastasis of colorectal cancer by targeting CRLF1. miR-3065-3p might serve as a promising prognostic marker as well as a therapeutic target for colorectal cancer.

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Urine miRNA Signature as a Potential Non-invasive Diagnostic and Prognostic Biomarker in Cervical Cancer

Cited by 7 publications

References 51 publications

Disease progression role as well as the diagnostic and prognostic value of microRNA-21 in patients with cervical cancer: A systematic review and meta-analysis

Disease progression role as well as the diagnostic and prognostic value of microRNA-21 in patients with cervical cancer: A systematic review and meta-analysis

O uso de microRNAs como biomarcadores do câncer cervical

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

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