Urine Formaldehyde: A Non-Invasive Marker for Alzheimer’s Disease?

Tong, Zhiqian

doi:10.4172/2161-0460.1000345

Cited by 2 publications

(1 citation statement)

References 67 publications

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“…The mass fragments and the typical relative intensities of the reduced final product "DMPO-CHO" are: 93.068 (49], 79.054 (47], 98.096 (43], 109.063 (37], 108.078 (31], 77.038 (28], 91.053 (26], 82.064 (25], 69.070 (22], 84.081 (22], 53.038 (22], 56.0498 (21]. The reaction mechanisms of formation of DMPO-OH adduct were already described by Yang et al [41].…”

Section: Mass Spectrometrymentioning

confidence: 99%

Detection of the formyl radical by EPR spin-trapping and mass spectrometry

Bauer

Hoque

Wolf

et al. 2018

Free Radical Biology and Medicine

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For the first time we here present the unambiguous identification of the formyl radical (CHO) by EPR (Electron Paramagnetic Resonance) spectroscopy and mass spectrometry (MS) using DMPO (5,5-dimethyl-1-pyrroline N-oxide) as spin trap at ambient temperature without using any catalyst(s). The CHO was continuously generated by UV photolysis in closed anoxic environment from pure formaldehyde (HCHO) in aqueous solution. The isotropic hyperfine structure constants ofCHO were determined as a = 15.72G and a = 21.27G. The signals were deconvoluted and split by simulation in their single adduct components: DMPO-CHO, DMPO-H and DMPO-OH. We verified our results at first using MNP (2-methyl-2-nitroso-propane) as spin trap with known literature data and then mass spectrometry. Similarly the MNP adduct components MNP-CHO, MNP-H as well as its own adduct, the MNP-2-methyl-2-propyl (MNP-MP) were deconvoluted. Due to the low signal intensities, we had to accumulate single measurements for both spin traps. Using MS we got the exact mass of the reduced CHO adduct independently confirming the result of EPR detection of formyl radical.

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