Urine – A waste or the future of regenerative medicine?

Kloskowski, Tomasz; Nowacki, Maciej; Pokrywczyńska, Marta; Drewa, Tomasz

doi:10.1016/j.mehy.2015.01.019

Cited by 14 publications

(12 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, recent studies have indicated that the cells isolated from voided urine or urine catheterized from urinary tract have multi-lineage differentiation capability which are highly expandable and posses stem cell features. [1,2,[10][11][12][13] One of the most important results of the present study is that that human urine contains mesenchymal stem cells together with urothelial cells. This result is in compliance with the results other studies published in recent years.…”

Section: Discussionmentioning

confidence: 60%

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Tayhan¹,

Keleş²,

Topçu³

et al. 2017

Turkish Journal of Urology

View full text Add to dashboard Cite

Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods:After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies.Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine-derived cell population. Conclusion:According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.

show abstract

Section: Discussionmentioning

confidence: 60%

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Tayhan¹,

Keleş²,

Topçu³

et al. 2017

Turkish Journal of Urology

View full text Add to dashboard Cite

show abstract

“…Isolation of UDSC from fresh urine is preferable, but protocols for UDSC isolation can be performed within 24 h if urine is stored at 4 • C in a storage medium with serum; longer storage negatively influences the viability of cells [30]. Recommendation on the process of urine collection, including exclusion criteria (e.g., viral or bacterial infections and malignancies) for future regenerative medicine, have been previously published; nevertheless, the future will bring advances in the automatic recovery of cells from the urine and possibly rescuing UDSC from otherwise excluded individuals [31].…”

Section: Morphological Characterization and Early Applications Of Urimentioning

confidence: 99%

Urine-Derived Stem Cells: Applications in Regenerative and Predictive Medicine

Bento

Шафигуллина

Rizvanov

et al. 2020

Cells

View full text Add to dashboard Cite

Despite being a biological waste, human urine contains a small population of cells with self-renewal capacity and differentiation potential into several cell types. Being derived from the convoluted tubules of nephron, renal pelvis, ureters, bladder and urethra, urine-derived stem cells (UDSC) have a similar phenotype to mesenchymal stroma cells (MSC) and can be reprogrammed into iPSC (induced pluripotent stem cells). Having simple, safer, low-cost and noninvasive collection procedures, the interest in UDSC has been growing in the last decade. With great potential in regenerative medicine applications, UDSC can also be used as biological models for pharmacology and toxicology tests. This review describes UDSC biological characteristics and differentiation potential and their possible use, including the potential of UDSC-derived iPSC to be used in drug discovery and toxicology, as well as in regenerative medicine. Being a new cellular platform amenable to noninvasive collection for disease stratification and personalized therapy could be a future application for UDSC.

show abstract

“…Urothelial differentiation of UDSCs presents an almost limitless cell source for tissue engineering or model fabrication with simple methods that can be used to isolate and expand urine cells. Additionally, the possibility of producing cells from urine samples makes the human urine-derived cells a smart choice for cell therapy [52]. This can produce high-quality cells, which can be expanded extensively [42].…”

Section: Advantages Of Udscsmentioning

confidence: 99%

Urine as a Main Effector in Urological Tissue Engineering—A Double-Edged Sword

Abbas

Ali

Uddin

2020

Cells

View full text Add to dashboard Cite

In order to reconstruct injured urinary tract tissues, biodegradable scaffolds with autologous seeded cells are explored in this work. However, when cells are obtained via biopsy from individuals who have damaged organs due to infection, congenital disorders, or cancer, this can result in unhealthy engineered cells and donor site morbidity. Thus, neo-organ construction through an alternative cell source might be useful. Significant advancements in the isolation and utilization of urine-derived stem cells have provided opportunities for this less invasive, limitless, and versatile source of cells to be employed in urologic tissue-engineered replacement. These cells have a high potential to differentiate into urothelial and smooth muscle cells. However, urinary tract reconstruction via tissue engineering is peculiar as it takes place in a milieu of urine that imposes certain risks on the implanted cells and scaffolds as a result of the highly cytotoxic nature of urine and its detrimental effect on both growth and differentiation of these cells. Both of these projections should be tackled thoughtfully when designing a suitable approach for repairing urinary tract defects and applying the needful precautions is vital.

show abstract

Urine – A waste or the future of regenerative medicine?

Cited by 14 publications

References 65 publications

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Urine-Derived Stem Cells: Applications in Regenerative and Predictive Medicine

Urine as a Main Effector in Urological Tissue Engineering—A Double-Edged Sword

Contact Info

Product

Resources

About