Urinary Xist is a potential biomarker for membranous nephropathy

Huang, Ya‐Fang; Hsieh, Hsin-Yi; Shih, Hsiu‐Ming; Sytwu, Huey‐Kang; Wu, Chia-Chao

doi:10.1016/j.bbrc.2014.08.077

Cited by 58 publications

(66 citation statements)

References 33 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HULC (highly upregulated in liver cancer) is another lncRNA that is associated with cancer. It can sequester and inhibit the function of several miRNAs, including the tumor suppressor miR-372 (Figure 1E)[47, 63] However, dysregulation of lncRNA is not only associated with several types of cancers but a variety of human diseases including: cardiovascular disease [64–66], diabetes [67], membranous nephropathy [68], neural pathogenesis such as autism spectrum disorder and Alzheimer’s disease [57, 69, 70], and alterations of both the innate and adaptive immune system [71, 72]. Moreover, recent studies have revealed that lncRNAs also are localized within extracellular vesicles (EVs) and may be important for long distant intercellular communication and function as biomarkers [73–76].…”

Section: Lncrnas Cancer and Other Diseasementioning

confidence: 99%

Environmental Health and Long Non-coding RNAs

Karlsson

Baccarelli

2016

Curr Envir Health Rpt

View full text Add to dashboard Cite

An individual’s risk of developing a common disease typically depends on an interaction of genetic and environmental factors. Epigenetic research is uncovering novel ways through which environmental factors such as diet, air pollution, and chemical exposure can affect our genes. DNA methylation and histone modifications are the most commonly studied epigenetic mechanisms. The role of long non-coding RNAs (lncRNAs) in epigenetic processes has been more recently highlighted. LncRNAs are defined as transcribed RNA molecules greater than 200 nucleotides in length with little or no protein-coding capability. While few functional lncRNAs have been well characterized to date, they have been demonstrated to control gene regulation at every level, including transcriptional gene silencing via regulation of the chromatin structure, and DNA methylation. This review aims to provide a general overview of lncRNA function with a focus on their role as key regulators of health and disease, and as biomarkers of environmental exposure.

show abstract

Section: Lncrnas Cancer and Other Diseasementioning

confidence: 99%

Environmental Health and Long Non-coding RNAs

Karlsson

Baccarelli

2016

Curr Envir Health Rpt

View full text Add to dashboard Cite

show abstract

“…However, the benchto-bedside application has not proven successful for any of these markers. Recently, urinary X-inactive specific transcript (Xist) was proposed as a potential biomarker of membranous nephropathy (13 ).…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNAs in Urine Are Detectable and May Enable Early Detection of Acute T Cell–Mediated Rejection of Renal Allografts

et al. 2015

View full text Add to dashboard Cite

BACKGROUND Long noncoding RNAs (lncRNAs) are novel intracellular noncoding ribonucleotides regulating the genome and proteome. They are detectable in the blood of patients with acute kidney injury. We tested whether lncRNAs are present in urine and may serve as new predictors of outcome in renal transplant patients with acute rejection. METHODS A global lncRNA expression analysis was performed with RNA from urine of patients with acute T cell–mediated renal allograft rejection and control transplant patients. Deregulated lncRNAs were confirmed in kidney biopsies and urine in a validation cohort of 62 patients with acute rejection, 10 of them after successful antirejection therapy, and 31 control transplant patients. RESULTS A global screen revealed several lncRNAs to be deregulated in urine of patients with acute rejection. Three intergenic lncRNAs, LNC-MYH13-3:1, RP11-395P13.3-001, and RP11-354P17.15-001, were most strongly altered. These were validated in the whole cohort of patients. RP11-395P13.3-001 and RP11-354P17.15-001 were upregulated in patients with acute rejection compared with controls. Only levels of RP11-354P17.15-001 normalized in patients with acute rejection after successful antirejection therapy. RP11-354P17.15-001 was associated with higher decline in glomerular filtration rate 1 year after transplantation. In vitro, in tubular epithelial cells, all lncRNAs were enriched by interleukin-6 treatment, but only RP11-395P13.3-001 and RP11-354P17.15-001 increased in cell culture supernatant, indicating that these lncRNAs might be secreted under inflammatory conditions. CONCLUSIONS lncRNAs are strongly altered in urine of patients with acute rejection. Urinary RP11-354P17.15-001 may serve as a novel biomarker of acute kidney rejection, identifying patients with acute rejection and predicting loss of kidney function.

show abstract

“…More recently, advances in the use of Xist expression have led to its potential as a biomarker attributing to its high levels in the urine of patients with membranous nephropathy (MN). MN is an autoimmuneinduced glomerular nephritis and the most common cause of nephrotic syndrome in humans (Huang et al 2014). The primary experiments carried out in MN model mice showed significant upregulation of Xist and long noncoding gene Neat1 in tubular epithelial and glomerular cells.…”

Section: Therapeutic and Diagnostic Applications Using X-inactivationmentioning

confidence: 99%

Understanding the Complex Circuitry of lncRNAs at the X-inactivation Center and Its Implications in Disease Conditions

Richard

Ogawa

2015

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Balanced gene expression is a high priority in order to maintain optimal functioning since alterations and variations could result in acute consequences. X chromosome inactivation (X-inactivation) is one such strategy utilized by mammalian species to silence the extra X chromosome in females to uphold a similar level of expression between the two sexes. A functionally versatile class of molecules called long noncoding RNA (lncRNA) has emerged as key regulators of gene expression and plays important roles during development. An lncRNA that is indispensable for X-inactivation is X-inactive specific transcript (Xist), which induces a repressive epigenetic landscape and creates the inactive X chromosome (Xi). With recent advents in the field of X-inactivation, novel positive and negative lncRNA regulators of Xist such as Jpx and Tsix, respectively, have broadened the regulatory network of X-inactivation. Xist expression failure or dysregulation has been implicated in producing developmental anomalies and disease states. Subsequently, reactivation of the Xi at a later stage of development has also been associated with certain tumors. With the recent influx of information about lncRNA biology and advancements in methods to probe lncRNA, we can now attempt to understand this complex network of Xist regulation in development and disease. It has become clear that the presence of an extra set of genes could be fatal for the organism. Only by understanding the precise ways in which lncRNAs function can treatments be developed to bring aberrations under control. This chapter summarizes our current understanding and knowledge with regard to how lncRNAs are orchestrated at the X-inactivation center (Xic), with a special focus on how genetic diseases come about as a consequence of lncRNA dysregulation.

show abstract

Urinary Xist is a potential biomarker for membranous nephropathy

Cited by 58 publications

References 33 publications

Environmental Health and Long Non-coding RNAs

Environmental Health and Long Non-coding RNAs

Long Noncoding RNAs in Urine Are Detectable and May Enable Early Detection of Acute T Cell–Mediated Rejection of Renal Allografts

Understanding the Complex Circuitry of lncRNAs at the X-inactivation Center and Its Implications in Disease Conditions

Contact Info

Product

Resources

About