Urinary Volatile Organic Compounds as Potential Biomarkers in Idiopathic Membranous Nephropathy

Wang, Mingao; Xie, Ruiqin; Jia, Xibei; Liu, Ruichan

doi:10.1159/000478782

Cited by 21 publications

(16 citation statements)

References 29 publications

(33 reference statements)

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“…CAN_1 variable markedly separated Y from the other three groups and the discriminant VOCs involved were 2-butanone, isopropyl alcohol, 2-pentanone, 3penten-1-ol, and pyrrole. These VOCs were detected in the urine of healthy subjects but also, in some cases with higher levels, in patients with different pathologies, such as cancer, nephropathy, and metabolic disorders (Silva et al 2012;Wang et al 2017;Mochalski et al 2018;Jia et al 2019). In our samples, these VOCs were more associated with Y group as they showed a higher correlation with CAN1.…”

Section: Discriminant Analysis Of Urinesmentioning

confidence: 48%

The smell of longevity: a combination of Volatile Organic Compounds (VOCs) can discriminate centenarians and their offspring from age-matched subjects and young controls

Conte

Martucci

et al. 2019

GeroScience

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Aging is characterized by dynamic changes at metabolic level that lead to modifications in the composition of the metabolome. Since the identification of biomarkers that can discriminate people of different age and health status has recently attracted a great interest, we wondered whether age-specific changes in the metabolome could be identified and serve as new and informative biomarkers of aging and longevity. In the last few years, a specific branch of metabonomics devoted to the study of volatile organic compounds (VOCs) has been developed. To date, little is known about the profile of specific VOCs in healthy aging and longevity in humans; therefore, we investigated the profile of VOCs in both urine and feces samples from 73 volunteers of different age including centenarians that represent useful "super-controls" to identify potential biomarkers of successful aging and footprints of longevity. To this purpose, we performed a discriminant analysis by which we were able to identify specific profiles of urinary and fecal VOCs. Such profiles can discriminate different age groups, from young to centenarians, and, even more interesting, centenarians' offspring from age-matched controls. Moreover, we were able to identify VOCs that are specific for the couples "centenariansoffspring" or the trios "centenariansoffspringspouse," suggesting the possible existence of a familiar component also for VOCs profile.

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Section: Discriminant Analysis Of Urinesmentioning

confidence: 48%

The smell of longevity: a combination of Volatile Organic Compounds (VOCs) can discriminate centenarians and their offspring from age-matched subjects and young controls

Conte

Martucci

et al. 2019

GeroScience

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“…As a developing novel tool for the non-invasive detection of various disease states in recent years, VOC analysis has been applied in different body fluids, such as blood, urine, and saliva [12][13][14][15]. VOC analysis is important in the diagnosis of respiratory infections as an accurate and rapid method and has clinical value in guiding the use of antibiotics [19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…Among them, gas chromatography-mass spectrometry (GC-MS) is a preferred method for the identification of pathogen markers with an appropriate sensitivity [11]. As a developing novel tool for non-invasive detection of various disease states in recent years, VOC analysis has been applied in different body fluids, such as blood, urine, and saliva [12][13][14][15], and it also can be applied to identify pathogens such as Escherichia coli, Staphylococcus aureus, Mycobacterium tuberculosis, Haemophilus influenzae, Streptococcus pneumoniae, rhinoviruses, influenza A virus and others [9,10,16]. Although there are a large number of clinical studies on the diagnosis of CNS infection such as cellular and chemistry parameters in CSF, conventional culture for bacteria and PCR with sequencing for viral [3,17] studies on the VOCs in the CSF have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds to Identify Infectious (Bacteria/Viruses) Diseases of the Central Nervous System - A Pilot Study

Qiu

Wang

et al. 2019

Preprint

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Background Central nervous system (CNS) infectious diseases are common diseases in emergency rooms and neurology departments. CNS pathogen identification methods are time-consuming and expensive and have low sensitivity and poor specificity. Some studies have shown that bacteria and viruses can produce specific volatile organic compounds (VOCs). The aim of this study is to find potential biomarkers by VOC analysis of cerebrospinal fluid (CSF) in patients with bacterial and viral meningitis/encephalitis (ME). Methods CSF samples from 16 patients with bacterial ME and 42 patients with viral ME were collected, and solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to analyze the metabolites in the CSF. Results There are two substances (Ethylene oxide and Phenol) that were found to be different between the two groups. Ethylene oxide was significantly greater in the group of bacterial ME patients than in the viral ME group of patients (P < 0.05). In addition, phenol was remarkably increased in the group of ME patients compared with the bacterial ME patients (P < 0.05). Conclusions Ethylene oxide and phenol may be potential biomarkers to distinguish bacterial ME and viral ME. VOC analysis of CSF may be used as a supporting tool for clinical diagnosis.

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“…Urine is frequently analyzed using Liquid Chromatography-Mass Spectrometry (LC-MS), Gas Chromatography-Mass Spectrometry (GC-MS) and Nuclear Magnetic Resonance Spectroscopy (NMR) [5][6][7]. A specific class of metabolomics focused on the profile of volatile compounds (VCs) is termed volatolomics, the applications of which for diagnostic purposes is growing [8][9][10][11][12][13][14][15]. VCs are secreted by cells of the human body, as a result of their metabolism.…”

Section: Introductionmentioning

confidence: 99%

“…The changes in the profile of VCs in biological fluids, dependent on the metabolic changes, may reflect the presence of disease. Many studies have suggested that the profile of VCs in urine change in cancer [8][9][10], nephrological conditions [15], oxidative stress [11], gastrointestinal diseases [12][13][14] and other disease states.…”

Section: Introductionmentioning

confidence: 99%

An Optimization of Liquid–Liquid Extraction of Urinary Volatile and Semi-Volatile Compounds and Its Application for Gas Chromatography-Mass Spectrometry and Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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Urinary volatile compounds (VCs) have been recently assessed for disease diagnoses. They belong to very diverse chemical classes, and they are characterized by different volatilities, polarities and concentrations, complicating their analysis via a single analytical procedure. There remains a need for better, lower-cost methods for VC biomarker discovery. Thus, there is a strong need for alternative methods, enabling the detection of a broader range of VCs. Therefore, the main aim of this study was to optimize a simple and reliable liquid–liquid extraction (LLE) procedure for the analysis of VCs in urine using gas chromatography-mass spectrometry (GC-MS), in order to obtain the maximum number of responses. Extraction parameters such as pH, type of solvent and ionic strength were optimized. Moreover, the same extracts were analyzed using Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), to evaluate the applicability of a single urine extraction for multiplatform purposes. After the evaluation of experimental conditions, an LLE protocol using 2 mL of urine in the presence of 2 mL of 1 M sulfuric acid and sodium sulphate extracted with dichloromethane was found to be optimal. The optimized method was validated with the external standards and was found to be precise and linear, and allowed for detection of >400 peaks in a single run present in at least 50% of six samples—considerably more than the number of peaks detected by solid-phase microextracton fiber pre-concentration-GC-MS (328 ± 6 vs. 234 ± 4). 1H-NMR spectroscopy of the polar and non-polar extracts extended the range to >40 more (mainly low volatility compounds) metabolites (non-destructively), the majority of which were different from GC-MS. The more peaks detectable, the greater the opportunity of assessing a fingerprint of several compounds to aid biomarker discovery. In summary, we have successfully demonstrated the potential of LLE as a cheap and simple alternative for the analysis of VCs in urine, and for the first time the applicability of a single urine solvent extraction procedure for detecting a wide range of analytes using both GC-MS and 1H-NMR analysis to enhance putative biomarker detection. The proposed method will simplify the transport between laboratories and storage of samples, as compared to intact urine samples.

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Urinary Volatile Organic Compounds as Potential Biomarkers in Idiopathic Membranous Nephropathy

Cited by 21 publications

References 29 publications

The smell of longevity: a combination of Volatile Organic Compounds (VOCs) can discriminate centenarians and their offspring from age-matched subjects and young controls

The smell of longevity: a combination of Volatile Organic Compounds (VOCs) can discriminate centenarians and their offspring from age-matched subjects and young controls

Volatile Organic Compounds to Identify Infectious (Bacteria/Viruses) Diseases of the Central Nervous System - A Pilot Study

An Optimization of Liquid–Liquid Extraction of Urinary Volatile and Semi-Volatile Compounds and Its Application for Gas Chromatography-Mass Spectrometry and Proton Nuclear Magnetic Resonance Spectroscopy

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