Urinary trypsin inhibitor attenuates the development of neuropathic pain following spinal nerve ligation

Gabexate mesilate ameliorates the neuropathic pain in a rat model by inhibition of proinflammatory cytokines and nitric oxide pathway via suppression of nuclear factor-κB

“…The sample size was calculated using "G*Power3" software (available freely at http://www.psycho.uni-duesseldorf.de/ abteilungen/aap/gpower3). The effect size was calculated as 0.77 according to the results of the previous study which used another serine protease inhibitor [2]. Using α = 0.05 with a power 95%, the total sample size was calculated as 30 for the statistical analysis of three consecutive measurements with PWT with repeated measures analysis of variance in three groups.…”

“…In terms of neuroinflammation, serine protease inhibitor has inhibitory properties on the proinflammatory cytokines and has the effect of attenuating the development of neuropathic pain [2,14]. Gabexate mesilate is one of those serine protease inhibitors with properties of antiinflammation and TNF-α inhibition by the suppression of NF-κB and the NO pathway [13,15,17] which are closely related with an inflammatory response and the development of neuropathic pain after spinal nerve injury.…”

“…After SNL, maximal increases of IL-1 and TNF-α were shown at the 3rd day after spinal nerve injury and decreased to control levels by the 14th day [46]. Thus, inflammatory cytokines might have the principal role in the initiation of neuroinflammation [2]. Moreover, maintenance of mechanical allodynia from neuropathic pain is also associated were significantly increased in the vehicle-treated group and the experimental group (group E) compared with the sham-operation group (P < 0.05).…”

“…with potent therapeutic effects according to its developmental mechanism [1]. Not only the changes in the nervous systems, but also neuroinflammation, are associated with the development of neuropathic pain [2].…”

“…Its property of TNF-α inhibition via monocyte also provides effectiveness in the treatment of sepsis-associated disseminated intravascular coagulation [13]. Recent studies have shown that the inhibitory effects of serine protease inhibitor on the proinflammatory cytokines can attenuate the development of neuropathic pain [2,14]. Gabexate mesilate also showed a protective effect after spinal cord trauma by inhibition of the increase in the myeloperoxidase activity in an animal model [15].…”