Urinary tract infections and antibiotic susceptibility among the patients attending B &amp; D hospital of Lalitpur, Nepal

Joshi, Yadav Prasad; Shrestha, Sneha; Kabir, Russell; Thapa, Arjun Kumar; Upreti, Parbati; Shrestha, Sanjit

doi:10.3126/ajms.v7i5.14908

Cited by 9 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Out of 1099 urine samples requested for urine culture, 21.7% showed significant bacterial growth. This was in correspondence to the similarly conducted study in Kathmandu valley, Nepal [17, 18]. Diabetic patients have higher odds of acquiring UTI compared to nondiabetic patients.…”

Section: Discussionsupporting

confidence: 88%

In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

Raya¹,

Belbase²,

Dhakal³

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Background. Diabetic patients are more susceptible to urinary tract infection compared to nondiabetic patients, Escherichia coli being the most common uropathogen causing UTI. Unreasonable and incorrect antibiotic prescription for UTI in these patients may induce the development of antibiotic-resistant urinary pathogens resulting in delayed recovery and longer hospitalization. In addition to these, biofilm forming capacity of the pathogen may worsen the problem. The main aim of this cross-sectional study (conducted from March to September 2015) is to detect the biofilm forming capacity of UTI causing micro-organisms and compare the antibiotic resistance pattern of Escherichia coli, the most common cause of UTI, which will help the physician in choosing the best antibiotic. Method. Total of 1,099 clean-catch mid stream urine (CCMSU) was processed by standard microbiological technique; 182 were from the diabetic group and 917 nondiabetic. Following identification, all isolates were subjected to antibiotic susceptibility testing using modified Kirby-Bauer disc diffusion method. In-vitro biofilm forming capacity of the isolates were detected by Microtitre plate method. The data were analyzed using SPSS software 16. Result. Urinary tract infection was found to be significantly higher in diabetic patients (42.9%) compared to nondiabetic patients (17.4%) with Escherichia coli as the most common uropathogen in both diabetic and nondiabetic groups. Similarly, UTI was more common in elderly population (29.5%). Imipenem, nitrofurantoin and amikacin were found to be the most effective drug for uropathogenic E. coli in both diabetic and nondiabetic patients, whereas amoxicillin, ciprofloxacin, and cotrimoxazole were least effective. Of the total bacterial isolates, 43.3% showed positive results for in-vitro biofilm production by the Microtitre plate method. A significantly higher resistance rate was observed among biofilm producing E. coli for quinolones, cotrimoxazole, and third generation cephalosporin ceftriaxone. Most of the biofilm producers (79.5%) were found to be MDR (p-value 0.015). Conclusion. Elderly populations with diabetes are at a higher risk of UTI. Higher biofilm production and resistance to in-use antimicrobial agents in this study render its inefficacy for empirical treatment and point out the importance of biofilm screening to ensure the effective management of infection.

show abstract

Section: Discussionsupporting

confidence: 88%

In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

Raya¹,

Belbase²,

Dhakal³

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The prevalence of UTI was found to be 17.20% which was lower than the studies by Kattel et al, and Rijal et al [25,26]. MSU specimens being collected from the patients under treatment, infections due to slow-growing bacteria or those which might not be able to grow in routine culture media used in the study might be the possible causes of a low rate of culture positivity [27].…”

Section: Discussionmentioning

confidence: 61%

“…The close proximity of the vaginal and anal opening in the female and the absence of the prostatic uid which has the bactericidal property in the female may make them more susceptible to the UTIs [29,31]. The patients of age group (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) showed the highest signi cant growth i.e. 44.49% which was similar to the study by Yadav The overall prevalence of UTI was 32.29% in diabetic patients and 15.97% in non-diabetic patients.…”

Section: Discussionmentioning

confidence: 99%

Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

Nayaju

Upreti

Ghimire

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The increased rate of urinary tract infection (UTI) in immunocompromised patients especially diabetic patients is a major public health problem in adults. Moreover, the infection with multidrug resistant strains producing extended spectrum beta-lactamase (ESBL) is a key obstacle in disease management among such vulnerable population. An immediate proper treatment depends on rapid diagnosis of UTI and screening of antimicrobial resistant pattern with highly sensitive methods which also reduces the possible urinary complications among the diabetic patients. Hence, this study was aimed to determine the occurrence of antibiotic resistant genes for β-lactamases; blTEM and blaCTX-M in uropathogenic Escherichia coli isolates from UTI suspected diabetic and non-diabetic patients. attendingMethods: A hospital-based cross-sectional study was conducted in Kathmandu Model Hospital in association with Central Department of Microbiology, TU from June to December 2018. A total of 1267 non-duplicate mid-stream urine specimens from diabetic and non-diabetic patients were obtained and processed immediately for isolation of uropathogens. The isolates were subjected for antibiotic susceptibility testing and ESBL confirmation. Finally, blaTEM and blaCTX-M ESBL genes were screened by using specific primers.Results: The overall prevalence of the urinary tract infection UTI was found to be 17.20%(218/1267) , out of which diabetic patients were significantly more infected with UTI accounting for 32.29%(31/96) as compared to non-diabetic persons, 15.97%(187/1171). A total of 221 bacterial were from 218 culture positive specimens in which E . coli was a most predominate one; 67.9%(150/221.). Forty-four percent (66/150) of the total E. coli was MDR and 37.33%(56/150) were ESBL producers. Among 56 isolates, 92.3%(12/13) from diabetic patents and 83.0% (44/53) were from non-diabetic patients. Furthermore, 84.85% of the screened ESBL producers were confirmed to possess either single or both of the blaTEM and blaCTX-M genes . The blaTEM and blaCTX-M genes were detected in 53.57% and 87.5% of the phenotypically ESBL confirmed E. coli .Conclusions: The UTI infection is an increasing problem in diabetic patients and infection with multidrug resistant strains specially ESBL producing uropathogens are causing a huge problem in disease management leading to high rate of mortality and morbidity of diabetic patients.

show abstract

“…E. coli isolated in (53%) followed by Enterococcus faecalis (13%), Staphylococcus aureus (11%), Klebsiella pneumonia (7%). A study done by (Joshi et al, 2016) found E. coli (66.7%) followed by Enterococcus (7.55%) and Staphylococcus (6.60%) causing UTI. This study was similar to our study by the prevalence of major uropathogens, but variation in percentage might be due to different places of studies.…”

Section: Resultsmentioning

confidence: 97%

Study of Culture and Sensitivity Pattern In Urinary Tract Infections in A Tertiary Care Center in Nepal

Chapagain¹,

Bhandari²,

Aryal³

2018

Med.Lab.Tech.J.

View full text Add to dashboard Cite

The main purpose of this study was to find out the causative agents of urinary tract infections (UTI) and their culture and antibiotic sensitivity in patients visiting Tribhuvan University and Teaching Hospital (TUTH). A retrospective study conducted among 155 patients, aged from 25-50 years with culture-positive UTI, who visited TUTH from 1st April 2017 to 30th September 2017. A culture of midstream urine was done to find out causative agents and their antibiotic sensitivity performed. Data were evaluated using Microsoft Excel 2016. Female were more affected than males. Escherichia coli (E. coli) was the most common microbes causing UTI in 53% patients. Most of the isolates on culture were Multi-Drug Resistant (MDR) strains to comprise 52%. Of the total gram-negative organisms, 33.9% were Extended Spectrum β-lactamase (ESBL) producers, and 3.57% were Metallo β-lactamase (MBL) producers. 29.41% of Staphylococcus were resistant to methicillin. E.coli is the most common organism causing UTI among adults. Multidrug-resistant has appeared alarming with resistant to most of the first line antibiotics.

show abstract

Urinary tract infections and antibiotic susceptibility among the patients attending B & D hospital of Lalitpur, Nepal

Cited by 9 publications

References 14 publications

In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

Study of Culture and Sensitivity Pattern In Urinary Tract Infections in A Tertiary Care Center in Nepal

Contact Info

Product

Resources

About