Urinary Tract Infection Caused by Corynebacterium Group D2: Report of 82 Cases and Review

Soriano, Francisco; Aguado, José María; Ponte, Carmen; Fernández-Roblas, R.; Rodríguez-Tudela, Juan Luis

doi:10.1093/clinids/12.6.1019

Cited by 94 publications

(102 citation statements)

References 27 publications

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“…Colonization of the skin of hospitalized patients is the probable source of infection [11]. The clinical aspects of CD2 infection have been reviewed by Soriano and Fernandez-Roblas [4,12] and by Coyle and Lipsky [13] but there have as yet, been no attempts to type these bacteria.…”

Section: Introductionmentioning

confidence: 99%

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Soto

Pitcher²,

Soriano

1991

Epidemiol. Infect.

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SUMMARYRestriction digest fragments of DNA from 46 clinical isolates identified as Corynebacterium group D2, were separated by electrophoresis, Southern blotted onto nylon membranes and hybridized to a ribosomal RNA gene probe. The resulting band patterns were subjected to unweighed pair-group cluster analysis. Representative strains from the main clusters were compared with similarly prepared band patterns from type strains of human Corynebacterium species.The results indicate that strains identified as Corynebacterium group D2 represent a unique taxon and that computer-assisted analysis of rRNA gene restriction fragment polymorphism (ribotyping) could be a useful technique in epidemiological studies of these bacteria.

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Section: Introductionmentioning

confidence: 99%

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Soto

Pitcher²,

Soriano

1991

Epidemiol. Infect.

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“…This group has tentatively been referred to as Corynebacterium urealyticum (23), but this name has not been validly published previously. Strains identified as C. urealyticum have been associated with cases of encrusted cystitis and other urinary tract infections in hospital patients (20), where they have been highly resistant to antibiotics (22). The natural habitat of these strains is human skin (24), but they readily colonize urinary tracts (22); strains have also been isolated from blood (14,17) and wounds (28).…”

mentioning

confidence: 99%

Classification of Coryneform Bacteria Associated with Human Urinary Tract Infection (Group D2) as Corynebacterium urealyticum sp. nov.

Pitcher¹,

Soto

Soriano

et al. 1992

International Journal of Systematic Bacteriology

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M u r~i a ,~ SpainUrealytic strains of coryneform bacteria that are designated Corynebacterium group D2 and are isolated from human urine are a cause of urinary tract infections. Cell wall and lipid analyses confirmed that these organisms are members of the genus Corynebacterium but can be separated from other species in the genus on the basis of DNA base composition and DNA-DNA hybridization values. Biochemically, strains in this taxon can be distinguished from other Corynebacterium spp. by their failure to produce acid from carbohydrates, by their failure to reduce nitrates, and by their ability to hydrolyze urea. We regard these bacteria as a new species of the genus Corynebacterium and propose the name Corynebacterium urealyticum. The type strain is strain NCTC 12011 (= ATCC 43042).The identification system for medically important coryneform bacteria devised by the Centers for Disease Control, Atlanta, Ga., designates one group of urealytic, nonfermentative bacteria as Corynebacterium group D2 and suggests that these organisms may be nitrate-negative variants of Corynebacterium pseudodiphtheriticum (9). This group has tentatively been referred to as Corynebacterium urealyticum (23), but this name has not been validly published previously. Strains identified as C. urealyticum have been associated with cases of encrusted cystitis and other urinary tract infections in hospital patients (20), where they have been highly resistant to antibiotics (22). The natural habitat of these strains is human skin (24), but they readily colonize urinary tracts (22); strains have also been isolated from blood (14, 17) and wounds (28). C. urealyticum is relatively inactive in conventional tests apart from its ability to hydrolyze urea rapidly. Colonially and microscopically, this species resembles Corynebacterium jeikeium, with which it shares a common habitat and resistance to antibiotics (4). However, the latter species is consistently nonurealytic and acidifies glucose and maltose. Evidence from lipid analysis (7, 21), from pyrolysis-mass spectrometry (8), and from rRNA gene restriction fragment polymorphism studies (26) indicates that there are clear differences between the two species. Chemotaxonomic evidence that group D2 is related to the genus Corynebacterium has been provided by Herrera-Alcarez et al. ("), who determined that the cell wall type is chemotype IV, with mycolic acids having chain lengths of 26 to 36 carbon atoms, and that the isoprenoid quinone MK-9(H2) is present.In this study, we estimated the DNA base compositions of Corynebacterium strains and levels of DNA-DNA relatedness between strains of C. urealyticum and other Corynebacterium species; we concluded that this taxon merits status as a new species in the genus Corynebacterium.* Corresponding author. MATERIALS AND METHODSBacterial strains and culture. The strains used in this study are listed in Table 1. Strains identified as C. urealyticum were isolated in Madrid, Spain, from the urine (strains A516/89, A524190, A532/90, A548/90, and NCTC 12011T [T =...

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“…The clinical significance of C. tuberculostearicum isolates originating from patients 9 and 12 is questionable because limited patient data are available and none of the clinical criteria or the criteria described by Funke and Bernard (10) were met. Therefore, it is probable that C. tuberculostearicum may be found as a frequent colonizer on the skin of hospitalized patients, causing or not causing infection, which has already been reported for other lipophilic and potentially multiresistant corynebacteria like C. jeikeium and C. urealyticum (9,19,20,21).…”

Section: Discussionmentioning

confidence: 85%

Corynebacterium tuberculostearicum: a Potentially Misidentified and Multiresistant Corynebacterium Species Isolated from Clinical Specimens

Hinić

Lang²,

Weisser

et al. 2012

J Clin Microbiol

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c Corynebacterium tuberculostearicum is a lipophilic corynebacterium validly characterized in 2004. We provide clinical information on 18 patients from whom this organism was isolated. The majority of the patients were hospitalized and had a history of prolonged treatment with broad-spectrum antimicrobials. In 7 (38.9%) of the 18 cases, the isolates were found to be clinically relevant. The present report also includes detailed data on the biochemical and molecular identification of C. tuberculostearicum, as well as its identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Our data demonstrate that routine biochemical tests do not provide reliable identification of C. tuberculostearicum. MALDI-TOF MS represents a helpful tool for the identification of this species, since all of the strains matched C. tuberculostearicum as the first choice and 58.3% (7/12) of the strains processed with the full extraction protocol generated scores of >2.000. Nevertheless, partial 16S rRNA gene sequencing still represents the gold standard for the identification of this species. Due to the challenging identification of C. tuberculostearicum, we presume that this organism is often misidentified and its clinical relevance is underestimated. The antimicrobial susceptibility profile of C. tuberculostearicum presented here reveals that 14 (87.5%) of the 16 strains analyzed exhibited multidrug resistance.

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Urinary Tract Infection Caused by Corynebacterium Group D2: Report of 82 Cases and Review

Cited by 94 publications

References 27 publications

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Classification of Coryneform Bacteria Associated with Human Urinary Tract Infection (Group D2) as Corynebacterium urealyticum sp. nov.

Corynebacterium tuberculostearicum: a Potentially Misidentified and Multiresistant Corynebacterium Species Isolated from Clinical Specimens

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