Urinary sex steroid hormone and placental leucine aminopeptidase concentration differences between live births and stillbirth of Bornean orangutans (<i>Pongo pygmaeus</i>)

Kinoshita, Kodzue; Sano, Yusuke; Takai, Akira; Shimizu, Mika; Kobayashi, Toshio; Ouchi, Akihiro; Kuze, Noko; Inoue‐Murayama, Miho; Idani, Gen’ichi; Okamoto, Masanori; Ozaki, Yasuhiko

doi:10.1111/jmp.12249

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…To date, a few studies in non-human animals have shown that LNPEP concentrations are higher during pregnancy in sheep (Atakisi et al, 2007), mice (Matsumoto & Mori, 1998), and orangutans (Kinoshita et al, 2017) which agrees with our results. In humans, it has been reported that maternal serum LNPEP levels are decreased in patients with hypertensive disorders, and gestational diabetes mellitus and can be used to predict pregnancy and perinatal mortality (Kozaki et al, 2001;Tian et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Yokus,

Takci,

Ercan

et al. 2024

Reprod Domestic Animals

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This study aimed to investigate maternal serum levels of some angiogenic factors and certain proteins in dairy cattle for (1) early prediction of unsuccessful fertilization and (2) early detection of possible pregnancy failures (early EM) after positive insemination Serum samples were collected from the same cattle at three distinct time points: 30 days before artificial insemination (B‐AI), on the day of artificial insemination (AI), and 30 days after artificial insemination (A‐AI). As a result of the pregnancy examination, the cows were divided into two main groups according to whether they were pregnant. The results showed that leucyl/cystinyl aminopeptidase (LNPEP) concentration was significantly decreased B‐AI and Secreted frizzled‐related proteins (SFRP‐3), Vascular Endothelial Growth Factor (VEGF) and LNPEP levels were significantly decreased on day of AI, while PRL level was increased, and these data have prognostic significance as early indicator of the risk of potentially failed pregnancy. Additionally, a significant decrease in LNPEP, SFRP3, and VEGF levels, along with an increase in PRL levels was also observed in A‐AI. These results suggest that these biomarkers can be used as a screening test to monitor the course of pregnancy. There were no significant differences in serum levels of Insulin‐Like Growth Factor 2 (IGF‐2), Tissue inhibitors of metalloproteinases (TIMP‐1), angiopoietin (ANG), Endoglin (ENG), Fibroblast growth factor (FGF), Inhibine‐A (INH‐A) and Transforming growth factors‐β1 (TGF‐β1) between the evaluated periods neither unsuccessful nor the successful pregnancy groups. This is the first study reporting that the maternal serum levels of LNPEP, SFRP3, VEGF, and PRL have important roles in pregnancy success and may indicate whether insemination outcome will be successful B‐AI and predict the risk of unsuccessful pregnancy after AI in dairy cattle. The increase in such studies will allow the development of more specific, practical, and applicable markers.

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Section: Discussionsupporting

confidence: 92%

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Yokus,

Takci,

Ercan

et al. 2024

Reprod Domestic Animals

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“…Therefore, serum P-LAP level has been reported to be a viable predictor of fetal death (Tian et al, 2016). In female Bornean orangutans that gave stillbirths, the P-LAP concentration in urine failed to increase; whereas in those that gave live births, the average P-LAP concentration in urine showed a progressive increase till delivery (Kinoshita et al, 2017). P-LAP is believed to prevent the premature onset of uterine contractions by degrading oxytocin, thereby playing a role in the maintenance of normal pregnancy (Yamahara et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Reciprocal Expression Patterns of Placental Leucine Aminopeptidase/Insulin-Regulated Aminopeptidase and Vasopressin in the Murine Brain

Goto

Nakamura

Ogawa

et al. 2020

Front. Mol. Biosci.

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Placental leucine aminopeptidase/insulin-regulated aminopeptidase (P-LAP/IRAP) regulates vasopressin and oxytocin levels in the brain and peripheral tissues by controlled degradation of these peptides. In this study, we determined the relationship between P-LAP/IRAP and vasopressin levels in subregions of the murine brain. P-LAP/IRAP expression was observed in almost all brain regions. The expression patterns of P-LAP/IRAP and vasopressin indicated that cells expressing one of these protein/peptide were distinct from those expressing the other, although there was significant overlap between the expression regions. In addition, we found reciprocal diurnal rhythm patterns in P-LAP/IRAP and arginine vasopressin (AVP) expression in the hippocampus and pituitary gland. Further, synchronously cultured PC12 cells on treatment with nerve growth factor (NGF) showed circadian expression patterns of P-LAP/IRAP and enzymatic activity during 24 h of incubation. Considering that vasopressin is one of the most efficient peptide substrates of P-LAP/IRAP, these results suggest a possible feedback loop between P-LAP/IRAP and vasopressin expression, that regulates the function of these substrate peptides of the enzyme via translocation of P-LAP/IRAP from intracellular vesicles to the plasma membrane in brain cells. These findings provide novel insights into the functions of P-LAP/IRAP in the brain and suggest the involvement of these peptides in modulation of brain AVP functions in hyperosmolality, memory, learning, and circadian rhythm.

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“…。しかし，尿は排泄後に時間が経過すると乾燥して回収できなかったり，排尿場所によっては全く回収ができない場合がある。そのため，より採取が容易な糞を用いた研究が，その後 1990 年頃から飼育下および野生下で発表されるようになった (Lasley and Kirkpatrick, 1991;Wasser et al, 1988;Ziegler et al, 1989) 。雌の性ホルモン(特に種特異性のない性ステロイドホルモン)濃度を分析することで，春機発動(繁殖能力の発現開始) ，発情期(排卵期) ，妊娠の有無，陰部腫脹との関係などが明らかにされてきた (Carroll et al, 1990;Curtis et al, 2000;Dahl et al, 1987;Heistermann et al, 1996;Kinoshita et al, 2017;Shimizu, 2005;Shimizu et al, 2003) Fourie et al, 2016) Fairbanks et al, 2011;Henley et al, 2013;Vliegenthart et al, 2016;Yamanashi et al, 2013) (Maggioncalda et al, 1999;Marty et al, 2015) ，その間に成長ホルモンの濃度も上昇することが確認されている (Maggioncalda et al, 2000) 。ただし，野生オランウータンにおける遺伝解析により，アンフランジ(フランジがない)雄でも子の父親になっていることが確認されており (Goossens et al, 2006) (Prudom et al, 2007;Roberts et al, 2001;Soltis et al, 2005;Ziegler and Snowdon, 2000;Ziegler et al, 1996) (Woods, 1975) Pettitt et al, 2007) 。糞中の水分含量が高いと，より一層細菌による代謝は活発になる (Palme, 2005 (Khan et al, 2002;Palme, 2005;Pettitt et al, 2007;Woods, 1975 (Kinoshita et al, 2016) 。オランウータンやチンパンジーにおいて，有機溶媒を用いてフィルターペーパーからホルモンを抽出し分析に成功している研究例もある (Seraphin et al, 2008;Shideler et al, 1995;Thompson and Knott, 2008) 。このように，調査地の状況に合わせてどのような保存方法をとるべきかについても，予備実験で確認しておくことが重要である。また，近年は「Field-Friendly 法」として，保存過程を経ずに野外で直接サンプルからホルモンを抽出し実験室に抽出物を持ち帰って分析している例もある (Nugr...…”

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