Urinary pseudouridine/creatinine ratio as an indicator of gastrointestinal cancer

Abstract

Urinary pseudouridine/creatinine ratio was determined in 74 patients with gastrointestinal tumours and 34 patients with no known malignant disease. The reproducibility of a single random urine sample was demonstrated. The mean ratio for control patients was 26.9 +/- 7.7 nmol/mumol and no control patient exceeded the mean by 2 standard deviations. There was no difference in the ratio between the sexes. Sixty-five per cent of colon cancer patients and 37.5 per cent of gastric and rectal cancer patients exceeded this upper limit of normality. There was no correlation between pseudouridine/creatinine ratio and histological differentiation, liver involvement or stage in either colorectal or gastric cancer patients. Urinary pseudouridine/creatinine ratio is one of the better non-specific cancer markers and may be particularly useful for detecting colonic cancer.

Serum metabolomics reveals many novel metabolic markers of heart failure, including pseudouridine and 2-oxoglutarate

“…Tumour cells exhibit an unusually high turnover, and consequently it has also been proposed as a tumour marker (e.g. (Higley et al, 1982;Salvatore et al, 1983;Russo et al, 1984;Tamura et al, 1987;Amuro et al, 1988;Xu et al, 2000;Zheng et al, 2005)) where it can have significant prognostic value (Pane et al, 1993). In heart failure its raised level may in part reflect the remodelling process in the heart itself (Morgan et al, 1987), the exact dynamics of which are unknown, but a substantially raised turnover of peripheral cells including skeletal muscle seems a more likely explanation.…”

Multivariate analyses as aids to diagnosis and assessment of prognosis in gastrointestinal cancer

“…Acute phase reactant proteins-C-reactive protein (CRP), a,-acid glycoprotein (AGP) and alantichymotrypsin (ACT)-were measured by single radial immunodiffusion using the method of Mancini et al (1965) using antisera and standards obtained from Behringwerke, Marburg/Lahn, Germany. Serum pseudouridine (i/) concentration was measured by high performance liquid chromatography by the method described by Davis et al (1977) and modified by Higley et al (1982).…”