Urinary pseudouridine as a tumor marker in patients with small cell lung cancer

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

“…affinity gel chromatography and separated by RP-HPLC [4,5,9,[15][16][17][19][20][21]. These clean-up procedures for urinary nucleosides need manual operation, resulting in artificial error and time consuming.…”

“…Therefore, any disorder of RNA turnover alters the levels of modified nucleosides. A number of studies have examined their biomedical significance as possible biomarkers for cancer [3][4][5][6][7][8][9], AIDS [10][11][12] and for the whole-body turnover of RNAs [13,14]. In addition, urinary nucleosides also have been used to monitor progress of disease and response to therapy [4,[15][16][17][18].…”

“…Several methodologies, including gas chromatography (GC) [3], reversed-phase high-performance liquid chromatography (RP-HPLC) [4,5,9,[15][16][17][19][20][21], capillary electrophoresis (CE) [7][8][9]22,23] and immunoassays [6,[24][25][26] have been used for the analysis of urinary nucleosides. Although immunoassays allow rapid analysis of hundreds of samples a day, the lack of specificity is of concern in evaluating the accuracy of the quantitative data.…”

Comparison of Serum and Urinary Levels of Modified Nucleoside, l-Methyladenosine, in Cancer Patients Using a Monoclonal Antibody-Based Inhibition ELISA.

“…This assay system seemed to have a sufficient sensitivity for determining the serum 1-methyladenosine because the serum 1-methyladenosine level of healthy individuals would be around 10 ng/ml from the estimation based on the reports of serum pseudouridine (Tamura et al 1987;Amuro et al 1988) and our urinary pseudouridine (Itoh et al 1989a) and 1-methyladenosine (Itoh et al 1988).…”

Age-dependence of urinary normal and modified nucleosides in childhood as determined by reversed-phase high-performance liquid chromatography

“…Consequently, in many pathological states the concentrations of all nucleosides as well as their relative patterns can be changed, especially in malignant diseases, in which increased t-RNA turnover and enzyme alterations can occur in the malignant cells. Therefore, for many years modified nucleosides have been suggested as tumor markers [6][7][8][9][10][11][12].…”