Urinary podocyte-associated mRNA profile in Egyptian patients with diabetic nephropathy

Tohamy, Iman Abdul Rahman; Kahla, Hala; Elsayed, Naglaa M.; Ansary, Mervat El; Saadi, Gamal

doi:10.1016/j.dsx.2019.07.048

Cited by 7 publications

(12 citation statements)

References 19 publications

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“…Podocalyxin (PCX) is the central component of glycocalyx that imparts a high degree of negative charge on the podocyte surface to maintain podocyte architecture and modify the structure of slit diaphragms, suggesting its critical role in supporting the structural and functional integrity of the glomerular filtration barrier. 21 Evidence has shown that urine PCX in DN patients is higher than in healthy individuals, suggesting the use of increased urine PCX as a marker for podocyte damage in early DN and target of DN treatment. 22 Nephrin is a secretory protein in the slit diaphragm that reduces podocyte apoptosis and supports the glomerular filtration barrier by modulating the podocyte architecture, as well as relevant signaling pathways, and interacting with Podocin and CD2AP.…”

Section: Discussionmentioning

confidence: 99%

Effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN)

Liu¹,

Guo²,

et al. 2022

Pak J Med Sci

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Objectives: To investigate the effects of a glucagon-like peptide-1 receptor agonist (GLP-1RA) liraglutide on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN). Methods: Eighty-four DN patients treated by the department of endocrinology of the Affiliated Hospital of Hebei University during December 2017 and March 2019 were randomly assigned to a control group and a treatment group (n=42, respectively), with the control group prescribed with conventional DN medications and the treatment group receiving liraglutide treatment in addition to the conventional therapy. The course of treatment lasted for 12 weeks. hemoglobin A1c (HbA1C), body mass index (BMI), total cholesterol (TC), triglyceride (TG), urinary albumin excretion rate (UAER), urine podocalyxin (PCX), urine nephrin, as well as inflammation and oxidative stress markers such as tumor necrosis factor α (TNF-α), monocyte chemotactic protein-1 (MCP-1), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) were measured pre- and post-treatment for intergroup comparison. Results: After 12 weeks of treatment, HbA1C, BMI, TC, and TG in both groups were reduced in comparison with the pre-treatment levels, with the levels in the treatment group lower than in the control group (p<0.05); reduced levels of UAER, PCX, and nephrin were detected in the two groups, with the treatment group exhibiting a significant reduction in these markers compared with the control group (p<0.05); the 12-week treatment led to decreases in the TNF-α, MCP-1, and MDA levels in both groups, with the decline in the treatment group exceeding that in the control group, whereas both groups had an increased level of GSH-Px, with the level in the treatment group higher than that in the control group, and the differences were statistically significant (p<0.05, respectively). Conclusions: Liraglutide protects the kidneys and improves DN by inhibiting inflammation and oxidative stress, reducing urinary albumin excretion and podocyte damage and supporting renal function in addition to its hypoglycemic properties. doi: https://doi.org/10.12669/pjms.38.5.4719 How to cite this:Liu J, Guo S, Li H, Liu X. Effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN). Pak J Med Sci. 2022;38(5):---------. doi: https://doi.org/10.12669/pjms.38.5.4719 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN)

Liu¹,

Guo²,

et al. 2022

Pak J Med Sci

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“…Many studies have demonstrated an association of podocyturia and mRNA expression of podocyte proteins in urinary sediment with an activity and progression of different glomerulopathies, including adult diabetic nephropathy [ 27 ] and lupus nephropathy [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children

Medyńska

Chrzanowska

Kościelska‐Kasprzak

et al. 2021

JCM

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Introduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible, so it is important to find a useful marker of early damage. The study aimed to evaluate the albumin-to-creatinine ratio (ACR), urinary alpha-1-acid glycoprotein (α1-AGP), and mRNA of podocyte-specific proteins as indicators of glomerular injury and their relationship with the degree of obesity and metabolic disorders. Materials and Methods: A total of 125 obese children and 33 healthy peers were enrolled. Patients were divided into two groups, depending on SDS BMI values. ACR, α1-AGP, mRNA expression of nephrin, synaptopodin, podocin, and C2AP protein in urine sediment were measured. Results: ACR values did not differ between groups and were within the normal range. α1-AGP and mRNA expression were significantly higher in obese children compared with controls. mRNA expression of the remaining podocyte proteins was similar in both groups. No significant differences concerning all examined parameters were found depending on the degree of obesity. There was a positive significant correlation between α1-AGP and ACR. Conclusions: Increased α1-AGP before the onset of albuminuria suggests its usefulness as a biomarker of early glomerular damage in obese children. An increased podocin mRNA expression also indicates podocyte damage and may be linked to ORG development. The lack of increase in expression of other podocyte proteins suggests that podocin mRNA may be a more specific and sensitive biomarker. The degree of obesity has no impact on the tested parameters, but further studies are needed to confirm it.

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“…Another study looked into podocyte-associated markers: NPHS1 , NPHS2 , and PODXL [ 73 ]. Renal podocyte injury is pathologically significant in DN progression, so podocyte-associated genes might serve as monitoring biomarkers to evaluate kidney health.…”

Section: Transcriptomics Biomarkers Of T2dmentioning

confidence: 99%

“…Patients with macroalbuminuria had higher podocyte marker levels than normo- and microalbuminuria patients. These markers distinguished microalbuminuria patients from those without complications in univariate analyses, with AUCs of 0.685–0.961 [ 73 ].…”

Section: Transcriptomics Biomarkers Of T2dmentioning

confidence: 99%

Circulating Nucleic Acid-Based Biomarkers of Type 2 Diabetes

Padilla-Martínez

Wojciechowska

Szczerbiński

et al. 2021

IJMS

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Type 2 diabetes (T2D) is a deficiency in how the body regulates glucose. Uncontrolled T2D will result in chronic high blood sugar levels, eventually resulting in T2D complications. These complications, such as kidney, eye, and nerve damage, are even harder to treat. Identifying individuals at high risk of developing T2D and its complications is essential for early prevention and treatment. Numerous studies have been done to identify biomarkers for T2D diagnosis and prognosis. This review focuses on recent T2D biomarker studies based on circulating nucleic acids using different omics technologies: genomics, transcriptomics, and epigenomics. Omics studies have profiled biomarker candidates from blood, urine, and other non-invasive samples. Despite methodological differences, several candidate biomarkers were reported for the risk and diagnosis of T2D, the prognosis of T2D complications, and pharmacodynamics of T2D treatments. Future studies should be done to validate the findings in larger samples and blood-based biomarkers in non-invasive samples to support the realization of precision medicine for T2D.

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Urinary podocyte-associated mRNA profile in Egyptian patients with diabetic nephropathy

Cited by 7 publications

References 19 publications

Effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN)

Effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on podocytes, inflammation, and oxidative stress in patients with diabetic nephropathy (DN)

Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children

Circulating Nucleic Acid-Based Biomarkers of Type 2 Diabetes

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