Urinary Oligosaccharides of Fucosidosis

Abstract

Urine of a fucosidosis patient contained a large amount of fucosyl oligosaccharides and fucose-rich glycopeptides. Six major oligosaccharides were purified by a combination of Bio-Gel P-2 and P-4 column chromatographies and paper chromatography. Structural studies by sequential exoglycosidase digestion and by methylation analysis revealed that their structures were as follows: Fucalpha1 leads to 6GlcNAc, Fucalpha1 leads to 2Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 2Manalpha1 leads to 3Manbeta1 leads to 4GlcNAc, Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 4Manalpha1 leads to 4GlcNAc, Galbeta1 leads to 4(Fucalpha1 leads to3)GlcNAcbeta1 leads to 2Manalpha1 leads to 6Manbeta1 leads to 4GlcNAc, and Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 4Manalpha1 leads to 6Manalpha1 leads to 6Manbeta1 leads to 4GlcNAc. In additon, the structure of a minor decasaccharide was found to be Galbeta1 leads to (Fucalpha1 leads to)GlcNAcbeta1 leads to Manalpha1 leads to [Galbeta1 leads to (Fucalpha1 leads to)GlcNAcbeta1 leads to Manalpha1 leads to]Manbeta1 leads to 4GlcNAc.

Characterisation of oligosaccharides released from human‐blood‐group O erythrocyte glycopeptides by the endo‐β‐galactosidase of Bacteroides fragilis

“…Sodium b~ro [~H]hydride (527 Ci/mol) was from New England Nuclear, DuPont (Stevenage, UK). Dextran T2000 was from Pharmacia (Milton Keynes, UK); an acid hydrolysate of Dextran T2000 was obtained using the method of Nishigaki et al [15] and contained glucose oligomers of 1-20 glucose units.…”

Severe Infantile Sialidosis-The Characteristics of Oligosaccharides Isolated from the Urine and the Abdominal Ascites.

“…Glucose oligomers were obtained by partial acid hydrolysis of dextran, as described in (Nishigaki et al 1978). Preparation and labeling of oligosaccharide fractions.…”

“…This procedure converted the sialyl oligosaccharides to neutral oligosaccharides (Takahashi et al 1991). The structure of each neutral oligosaccharide were determined by sequential digestions using exoglycosidases and Bio-Gel P-4 column chromatography which were preformed under the special conditions of the previous paper (Nishigaki et al 1978). The location of the glycosidic linkage between each monosaccharide in the sialyl oligosaccharide chain was determined by means of the methylation analysis and the gas chromatographmass spectrometer (GC-MS) analysis (Takahashi and Orii 1989;Takahashi et al 1991).…”

Structural analysis of the asparagine-linked oligosaccharides of rat haptoglobin metabolically labeled in a hepatocyte culture system

“…Radioactive peaks obtained were compared with elution positions of glucose oligomers which were detected with a refractometer. Sizes of oligosaccharides were expressed as glucose units [23].…”