Urinary NGAL levels in potential deceased kidney donors may be useful in determining donor suitability

Proteomics and metabolomics in renal transplantation-quo vadis?

“…Proteogenomic approach, MALDI-TOF [118] Acute rejection Titin, lipopolysaccharide-binding protein, peptidase inhibitor 16, complement factor D, mannose-binding lectin, protein Z-dependent protease, b2 microglobulin, kininogen-1, afamin, serine protease inhibitor, phosphatidyl choline-sterol acyltransferase, sex hormone-binding globulin in plasma iTRAQ labeling in combination with LC-MS, ELISA [119] Acute rejection 66 Proteins in peripheral blood including NF-B, STAT 1 and STAT3 iTRAQ labeling in combination with LC-MS [120] Acute rejection Seven signals in urine, protein signals were characterized based on their mass/charge, but the proteins associated with these signals were not identified SELDI-TOF [121] Acute rejection Five signals in urine, signals were characterized based on their mass/charge, but the proteins associated with these signals were not identified SELDI-TOF [122] Acute rejection 45 Signals in urine of which 16 were considered molecular marker candidates, signals were characterized based on their mass/charge, but the proteins associated with these signals were not identified SELDI-TOF [123] Acute rejection Three signal clusters in urine, signals were characterized based on their mass/charge, but the proteins associated with these signals were not identified SELDI-TOF [124] Acute tubular injury b2 Microglobulin fragments in urine SELDI-TOF [125] have been examined in renal transplant patients: neutrophil gelatinase-associated lipocalin (NGAL) [139][140][141][142][143][144][145][146], kidney injury molecule-1 (KIM-1) [147][148][149][150], netrin-1 [151], interleukin-18 [146,152], a and п-glutathione S-transferase [153], liver fatty-acid-binding protein kidney [154], N-acetyl-b-D-glucosaminidase [143,155,156], b2-microglobulin and cystatin C. Most of these can be measured by ELISA or protein bead-based multi-analyte assays [81], and for some such as NGAL, assays on analytical platforms est...…”

“…In many cases as a result of nontargeted proteomic and genomic discovery studies , about two dozen potential markers of acute kidney injury have been identified . The following protein markers have been examined in renal transplant patients: neutrophil gelatinase‐associated lipocalin (NGAL) , kidney injury molecule‐1 (KIM‐1) , netrin‐1 , interleukin‐18 , α and п‐glutathione S ‐transferase , liver fatty‐acid‐binding protein kidney , N ‐acetyl‐β‐ d ‐glucosaminidase , β2‐microglobulin and cystatin C. Most of these can be measured by ELISA or protein bead‐based multi‐analyte assays , and for some such as NGAL, assays on analytical platforms established in clinical laboratories are either already available or under development. The potential impact of protein kidney dysfunction markers on kidney transplantation is reviewed in references .…”

Does the porcine model give us insight as to how can we improve renal transplantation from large donors to small recipients?

“…Urinary levels of neutrophil gelatinase‐associated lipocalin (NGAL) may be useful in determining the suitability of a potential deceased kidney donor (10). This study used NGAL as a biomarker of renal injury and found no significant difference in urinary NGAL between the small recipients and size‐matched control group.…”