Urinary miRNA profile for the diagnosis of IgA nephropathy

Abstract

Background IgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. Urinary micro-RNA (miRNA) level is increasingly reported to as non-invasive markers of various kidney diseases. We aim to identify urinary miRNA targets for the diagnosis of IgAN. Methods In the development cohort, we performed complete miRNA profiling of urinary sediment in 22 patients with IgAN and 11 healthy controls (CTL). Potential miRNA targets were quantified by a separate validation cohort of 33 IgAN patients and 9 healthy controls. Results In the development cohort, we identified 39 miRNA targets that have significantly different expression between IgAN and CTL (14 up-regulated, and 25 down-regulated). Among the 8 miRNA targets chosen for validation study, urinary miR-204, miR-431 and miR-555 remained significantly reduced, and urinary miR-150 level was significantly increased in the IgAN as compared to CTL. The area-under-curve of the receiver operating characteristic (ROC) curve for urinary mi-204 level for the diagnosis of IgAN was 0.976, and the diagnostic performance of combining additional miRNA targets was not further improved. At the cut-off 1.70 unit, the sensitivity and specificity of urinary miR-204 was 100 and 55.5%, respectively, for diagnosing IgAN. Conclusions Urinary miR-150, miR-204, miR-431 and miR-555 levels are significantly different between IgAN and healthy controls; urinary miR-204 level alone has the best diagnostic accuracy. Electronic supplementary material The online version of this article (10.1186/s12882-019-1267-4) contains supplementary material, which is available to authorized users.

Differential expression of microRNA miR-150-5p in IgA nephropathy as a potential mediator and marker of disease progression

“…However, due to the relatively small sample size, we did not have adequate statistical power to reliably assess statistical significance, this will require a much larger cohort (estimated 400-700 IgAN kidney biopsies). Importantly, these observations are consistent with two independent studies that have reported an association between miR-150-5p in the plasma 30 and in the urine 31 decline . [36][37][38][39][40][41][42] In addition to a potential role for miR-150-5p in determining infiltrating lymphocyte phenotype it is plausible that specific genes within the intrinsic kidney cell population are directly targeted by lymphocyte-derived miR-150-5p via the release of miR-containing exosomes.…”

Mean Expression Value Normalized and Absolute Quantified miR-21 Found to be a Potential Diagnostic and Prognostic Marker for IgA Nephropathy

“…This method reduced the group variance without changing the mean values. Previous studies have shown miR-155 expression level increased or decreased in urine, serum, and kidney tissue samples in IgAN, lupus nephritis, and other kidney disease cases [14,17,18,35]. We are the first one to report miR-155 as a potential reference gene in IgA nephropathy in plasma samples for quantitative PCR-based gene expression study.…”

The Non-Coding RNA Landscape in IgA Nephropathy—Where Are We in 2021?

“…In addition, both miRs -3613 and -4668 were downregulated in kidneys displaying no segmental sclerosis (S0 of the Oxford classification system) compared to the S1 lesion. Szeto et al [98] performed a similar study, employing the nanostring microarray platform. The group found a miR signature (miRs -204, -431, -555 (downregulated) and miR-150 (upregulated)) which was significantly differentially expressed in the urine sediments of IgAN patients compared to healthy subjects.…”