Urinary miR-210 as a Mediator of Acute T-Cell Mediated Rejection in Renal Allograft Recipients

Lorenzen, Johan M.; Volkmann, Ingo; Fiedler, Jan; Schmidt, Mascha; Scheffner, Irina; Haller, Hermann; Gwinner, Wilfried; Thum, Thomas

doi:10.1111/j.1600-6143.2011.03679.x

Cited by 183 publications

(133 citation statements)

References 22 publications

(25 reference statements)

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“…To further assess the diagnostic potential of miRNAs in this patient population, our group analyzed the miRNA expression profile in urine samples (quantitative reverse transcription PCR-based miRNA microarray) of patients with acute T cell-mediated renal allograft rejection (31). This approach was chosen to identify miRNAs deregulated in easily accessible urine to circumvent the potential risks associated with an invasive procedure such as a kidney biopsy.…”

Section: Circulating and Urinary Mirnas In Kidney Transplant Patientsmentioning

confidence: 99%

Circulating and Urinary microRNAs in Kidney Disease

Lorenzen

Thum

2012

Clinical Journal of the American Society of Nephrology

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Summary microRNAs are small, noncoding RNAs that control gene/protein expression through target messengerRNA degradation and/or inhibition of protein synthesis. An array of experimental studies has shown the importance of microRNAs for disease initiation/progression. microRNAs are generally considered to act as intracellular modulators of gene expression. However, first studies in the cancer and cardiovascular field have elucidated that microRNAs are remarkably stable in the extracellular compartment (e.g., in blood or urine). The detection and quantification of circulating microRNAs may, thus, represent a novel noninvasive tool to detect and monitor disease activity. In addition, there might be a potential biologic relevance of circulating microRNAs for cell/cell communication. The aim of the present article is to give an outline of recent work on circulating and urinary microRNAs as well as their potential paracrine activity in kidney disease.

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Section: Circulating and Urinary Mirnas In Kidney Transplant Patientsmentioning

confidence: 99%

Circulating and Urinary microRNAs in Kidney Disease

Lorenzen

Thum

2012

Clinical Journal of the American Society of Nephrology

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“…miRNAs in easily accessible body fluids could reflect tissue situation and are therefore of great, diagnostic and prognostic interest. Data regarding the detection of miRNAs in urine samples have been rarely published (12)(13)(14)(15)(16). In bladder cancer, urinary miR-126 was described as a specific indicator of BC (16), while overexpressed miR-96 and miR-183 were found to be correlated to tumour stage and grade (15).…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

et al. 2013

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Abstract. Since differential expression of microRNAs (miRNAs) has been found to be highly associated with several types of cancer, the goal of the present study was to identify an miRNA fingerprint as a non-invasive diagnostic tool to detect urinary bladder cancer using the easily accessible samples of whole blood and urine. Blood and urine samples from 4 controls and from patients suffering from superficial and invasive bladder cancer were analyzed using miRNA microarray consisting of 754 human miRNAs from the Sanger database v14. Using RT-qPCR technique, 6 of the differentially expressed miRNAs were validated in the controls (20 blood, 19 urine samples) and patients with superficial (18 blood, 16 urine samples) or invasive (20 blood and urine samples each) tumours. Three blood miRNAs (miR-26b-5p, miR-144-5p, miR-374-5p) were found to be significantly upregulated in invasive bladder tumour patients (P<0.05) when compared to the control group. The expression of 2 miRNAs (miR-618, miR-1255b-5p) in the urine of patients with invasive tumours was significantly (P<0.05) increased in comparison to the control group. Blood miR-26b-5p detected the presence of invasive bladder tumours with 94% specificity and 65% sensitivity. The urine miR-1255b-5p reached 68% specificity and 85% sensitivity in the diagnosis of invasive tumours. This pilot study represents the first characterization of an miRNA profile for urinary bladder tumours in whole blood samples. In addition, it was shown that invasive bladder tumours could be identified by differentially expressed urine miRNAs. Further studies are needed to test the clinical usefulness for bladder cancer detection and surveillance.

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“…A single study has addressed the association between urinary microRNA and acute T cell–mediated rejection 31. In this study, the urinary microRNA transcriptome profile was first examined in pooled urine samples of five patients with acute rejection and five patients with stable graft function.…”

Section: Urinary Micrornas As Biomarkers Of Graft Injurymentioning

confidence: 99%

Urinary MicroRNA as Biomarker in Renal Transplantation

Vrie

Deegens

Eikmans

et al. 2017

American Journal of Transplantation

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Urine represents a noninvasive source in which proteins and nucleic acids can be assessed. Such analytes may function as biomarkers to monitor kidney graft pathology at every desired frequency, thereby providing a time window to prevent graft damage by therapeutic intervention. Recently, several proteins have been measured in urine as markers of graft injury. However, the specificity is limited, and measuring urinary proteins generally lacks the potential to predict early kidney graft damage. Currently, urinary mRNA and microRNA are being investigated to evaluate the prognostic value of changes in gene expression during the initial stages of graft damage. At such time point, a change in treatment regimen and dosage is expected to have maximum potency to minimize future decline in graft function. Both mRNA and microRNAs have shown promising results in both detection and prediction of graft injury. An advantage of microRNAs compared to mRNA molecules is their stability, a characteristic that is beneficial when working with urine samples. In this review, we provide the current state of urinary biomarkers in renal transplantation, with a focus on urinary microRNA. In addition, we discuss the methods used to study urinary microRNA expression.

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Urinary miR-210 as a Mediator of Acute T-Cell Mediated Rejection in Renal Allograft Recipients

Cited by 183 publications

References 22 publications

Circulating and Urinary microRNAs in Kidney Disease

Circulating and Urinary microRNAs in Kidney Disease

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

Urinary MicroRNA as Biomarker in Renal Transplantation

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