Urinary Microbiome and Cytokine Levels in Women With Interstitial Cystitis

Abernethy, Melinda G.; Rosenfeld, Amy; White, James R.; Mueller, Margaret; Lewicky-Gaupp, Christina; Kenton, Kimberly

doi:10.1097/aog.0000000000001892

Cited by 111 publications

(92 citation statements)

References 25 publications

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“…Despite the study limitation of small sample size, our numbers were large enough to suggest that microbiota of the lower urinary tract may not contribute to the symptoms in women meeting the clinical definition of IC/PBS. This contradicts recent work by others, who have argued for a link between clinical symptoms of IC/PBS and the FUM 13 14 15 . Abernathy et al .…”

Section: Discussioncontrasting

confidence: 82%

“…Few groups have reported on the FUM of IC/PBS patients. The few existing studies used various urine collection methods and bacterial detection methods, in general had small sample sizes and, therefore, the data conflict 13 14 15 . Our prospective case-controlled study was designed to determine if the FUM of women with and without IC/PBS differs using mid-stream voided urine specimens to avoid pain provocation and analyzed them with expanded techniques.…”

Section: Introductionmentioning

confidence: 99%

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Female lower urinary tract microbiota do not correspond to IC/PBS symptoms: a case-controlled study

Bresler

Price

Tulke

et al. 2019

Preprint

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OBJECTIVE: Current etiology of interstitial cystitis/painful bladder syndrome (IC/PBS) is poorly understood and multifactorial. Recent studies suggest the female urinary microbiota (FUM) contribute to IC/PBS symptoms. This study was designed to determine if the FUM, analyzed using mid-stream voided urine samples, differs between IC/PBS patients and controls. MATERIALS AND METHODS: This prospective case-controlled study compared the voided FUM of women with symptoms of urinary frequency, urgency, and bladder pain for greater than 6 months to the voided FUM of healthy female controls without pain. Bacterial identification was performed using 16S rRNA gene sequencing and EQUC, a validated enhanced urine culture approach. Urotype was defined by a genus present at >50% relative abundance. If no genus was present above this threshold, the urotype was classified as mixed. Chi-square and Fishers exact tests were performed. P-values <0.05 were considered significant. RESULTS: A mid-stream voided specimen was collected from 21 IC/PBS patients and 20 asymptomatic controls. Both groups had similar demographics. Urotypes did not differ between cohorts as assessed by either EQUC or 16S rRNA gene sequencing. We detected no significant differences between cohorts in terms of alpha-diversity. Cohorts also were not distinct using Principle Component Analysis or hierarchical clustering. Detection by EQUC of bacterial species considered uropathogenic was high in both cohorts, but detection of these uropathogenic species did not differ between groups (p=0.10). CONCLUSIONS: Enhanced culture and modern DNA sequencing methods provide evidence that IC/PBS symptoms may not be related to differences in the FUM, at least not its bacterial components. Future larger studies are needed to confirm this preliminary finding.

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Section: Discussioncontrasting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Female lower urinary tract microbiota do not correspond to IC/PBS symptoms: a case-controlled study

Bresler

Price

Tulke

et al. 2019

Preprint

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“…1-4 While there are shared species in the urinary tract and those found in microbial communities associated with skin, gastrointestinal tract and vagina, studies currently suggest that the urinary tract contains microbial populations that are distinct from those at other sites of the human body that harbor microbiota. 1,5-7 Because the urinary microbiome was excluded from seminal studies such as HMP (Human Microbiome Project), which characterized the healthy human microbiota, 8 studies profiling healthy urinary microbiota are less mature, as is our understanding of how urinary microbial populations may shift with disease. 1 …”

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confidence: 99%

Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer

et al. 2018

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Purpose Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.

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“…In general, the core microbiota composition is very different between women and men. The urine of women is mainly dominated by Lactobacillus followed by Gardnerella genera[11,13,15–17]. Men have a more diverse bacterial composition consisting of broader representation of different genera including Lactobacillus , Corynebacterium , Staphylococcus , and Prevotella [13,18–23].…”

Section: Introductionmentioning

confidence: 99%

“…It is assumed that these bacteria provide healthy functioning of the lower urinary tract. Notably, several studies have recently documented that an alteration in urinary microbiota correlates to diseases in the lower urinary tract, including urgency urinary incontinence[10–12,24] and interstitial cystitis[15,25]. More knowledge on the urinary microbiota may therefore help us to understand the etiology behind diseases of the lower urinary tract.…”

Section: Introductionmentioning

confidence: 99%

The urinary microbiota composition remains stable over time and under various storage conditions

Bundgaard‐Nielsen

Ammitzbøll

et al. 2019

Preprint

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BackgroundNew sensitive techniques have revealed a large population of bacteria in the human urinary tract, challenging the perception of the urine of healthy humans being sterile. While the role of this urinary microbiota is unknown, dysbiosis has been linked to disorders like urgency urinary incontinence and interstitial cystitis. When comparing studies it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether storage condition or time of collection, had any impact on the urinary microbial composition.ResultsFor comparison of different storage conditions, urine was collected from five healthy adult female donors, and analyzed by 16S rRNA gene sequencing. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in nineteen healthy donors, including four women, five men, five girls, and five boys. With the exception of two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals. Conclusion: The composition of the urinary microbiota was found to be highly resilient to changes introduced by storage temperature and duration. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys.Together our study supports flexibility in study design, when conducting urinary microbiota studies.Author summaryThe discovery of bacteria native to the urinary tract in healthy people, a location previously believed to be sterile, has prompted research into the clinical potential of these bacteria. However, methodological weaknesses can significantly influence such studies, and thus development of robust techniques for investigating these bacteria are needed. In the present study, we investigated whether differences in storage following collection, could affect the bacterial composition of urine samples. Next, we investigated if this composition exhibited daily or day-to-day variations.Firstly, we found, that the bacterial composition of urine could be maintained by storage at −80 °C, −20 °C, or refrigerated at 4 °C. Secondly, the bacterial composition of urine remained stable over time. Overall, the results of this study provide information important to study design in future investigations into the clinical implications of urinary bacteria.

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Urinary Microbiome and Cytokine Levels in Women With Interstitial Cystitis

Cited by 111 publications

References 25 publications

Female lower urinary tract microbiota do not correspond to IC/PBS symptoms: a case-controlled study

Female lower urinary tract microbiota do not correspond to IC/PBS symptoms: a case-controlled study

Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer

The urinary microbiota composition remains stable over time and under various storage conditions

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