Urinary metallomics as a novel biomarker discovery platform: Breast cancer as a case study

Burton, Casey; Dan, Yongbo; Donovan, Ariel R.; Liu, Kun; Shi, Honglan; Ma, Yinfa; Bosnak, C. P.

doi:10.1016/j.cca.2015.11.014

Cited by 43 publications

(25 citation statements)

References 37 publications

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“…However, current understanding on the biochemistry and molecular pathology of mammalian pteridines remains fragmented [33]. As Rembold and Gyure presciently remarked over forty years ago, analytical limitations related to the general instability and trace levels of biogenic pteridines have precluded detailed studies of the prevalence and role of many lesser known derivatives [13,34].…”

Section: Introductionmentioning

confidence: 99%

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Burton

Shi

2016

Analytica Chimica Acta

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Pteridines have evoked considerable interest from the scientific community owing to their prominent roles in human health and disease. The availability of analytical methodologies suitable for comprehensive pteridine profiling, termed here as "pterinomics", has been limited by inconsistent sample preparation and the exclusion of lesser studied pteridine derivatives. In response, the present study describes a new pterinomics workflow using a high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) methodology for the simultaneous analysis of 15 pteridine derivatives including four structural isomers, marking the largest quantitative pteridine panel that has been studied to-date. The validated method possessed excellent sensitivity with method detection limits (0.025 μg L(-1) to 0.5 μg L(-1)) that were comparable or superior to existing techniques. Spiked recovery studies demonstrated the technique was both accurate (88-112%) and precise (RSD: 0-6%). A comparative study of commonly used oxidative pretreatments, including triiodide, permanganate, and manganese dioxide, revealed that the oxidative mechanisms were inefficient, complex, and concentration dependent. Finally, 50 clinical urine specimens were examined with the new technique wherein 10 pteridine derivatives were quantified and population ranges have been given. This technique can be used to examine pteridine molecular epidemiology and biochemistry to support related research applications, and may further be readily extended to include additional pteridine derivatives and biological matrices for specific applications.

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Section: Introductionmentioning

confidence: 99%

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Burton

Shi

2016

Analytica Chimica Acta

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“…As such, this difference primarily reects the SG-normalisation value of 1.020, which was also used in numerous previous investigations. 18,52,54,55 Notably, the SG-normalisation clearly reduces the dispersion of the individual results. In detail, the coefficients of variation (CV) for all elements, except As, are lower by between 10 to more than 50% for the SG-normalised compared to the non-normalised data ( Table 2).…”

Section: Sample Resultsmentioning

confidence: 99%

“…[5][6][7] Urine, a product of constant-rate kidney ltration of blood, represents a mixture of water-soluble waste metabolites. The study of urine (urinalysis) is well established and has been used to investigate exposure to hazardous elements or chemicals, [8][9][10][11][12] to identify abnormalities in absorption, [13][14][15][16][17] or for the study of diseases [18][19][20][21][22] to determine causes and improve prognosis. Compared to analyses of blood and faeces, urine analyses are relatively straightforward, as sample pre-treatment is facilitated by the relatively small amounts of organic and inorganic solutes that are present.…”

Section: Introductionmentioning

confidence: 99%

Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation

et al. 2018

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“…estimated that the MT level in breast cancer significantly increase due to higher metabolic activity of the tumor tissue and defense system against oxidative stress induced by chemotherapeutics. [13][14][15] The aim of our study was to design a chitosan based nanotransporter for DOX which was modified by zinc ions (Zn-ChNPs-DOX). Zinc ions were involved for higher uptake from the bloodstream via interaction with MT.…”

Section: Introductionmentioning

confidence: 99%

Zinc Modified Nanotransporter of Anticancer Drugs for Targeted Therapy: Biophysical Analysis

Skaličková

Docekalova

Staňková

et al. 2019

j nanosci nanotechnol

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Modern anticancer therapy aims to increase the effectiveness of tumor treatment. The aim of this work was to propose a new nanotransporter for targeted delivery of anthracycline antibiotics, which is characterized by its bioavailability, increased uptake of the drug from the bloodstream at the site of tumor tissue and as well as low toxicity to non-target tissue. Chitosan nanoparticles have attracted great attention in the field of drug delivery due to their stability, low toxicity and easy preparation. Deacetylated chitosan skeleton is composed of glucosamine units and has a high density of charged amino groups which allow strong electrostatic interactions with biomolecules, transition metals (Zn, Se) and peptides. We obtained the encapsulation effectiveness of chitosan 20%. Electrochemical detection of the bounded Zn 2+ ions into the chitosan structure showed shift from −0 99 to −0.93 V. This result proved the formation of a chitosan-zinc complex. The ability of metallothione in to quench the 2,2-diphenyl-1-picrylhydrazylradicalin the presence of 50 M doxorubicin was confirmed by the change of relative absorbance in the range of 50 to 60%.

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Urinary metallomics as a novel biomarker discovery platform: Breast cancer as a case study

Cited by 43 publications

References 37 publications

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation

Zinc Modified Nanotransporter of Anticancer Drugs for Targeted Therapy: Biophysical Analysis

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