Urinary metabonomic study of patients with acute coronary syndrome using UPLC-QTOF/MS

Wang, Yingfeng; Sun, Wenting; Zheng, Jilin; Xu, Can; Wang, Xia; Li, Tianyi; Tang, Yi‐Da; Li, Zhongfeng

doi:10.1016/j.jchromb.2018.10.005

Cited by 23 publications

(35 citation statements)

References 41 publications

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“…Oxidative stress is known to be involved in the pathogenesis of CVD [ 23 – 25 ], and prior metabolomics studies in human urine point to an important contribution for oxidative stress in acute coronary syndrome [ 26 ] and hypertension in persons < 50 years [ 27 ]. In the same line, our previous proteomic study highlighted oxidative stress as a major functional category that is altered by cardiovascular risk factors already present in young adults [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

et al. 2020

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The predictive value of traditional cardiovascular risk estimators is limited, and young and elderly populations are particularly underrepresented. We aimed to investigate the urine metabolome and its association with cardiovascular risk to identify novel markers that might complement current estimators based on age. Urine samples were collected from 234 subjects categorized into three age-grouped cohorts: 30–50 years (cohort I, young), 50–70 years (cohort II, middle-aged), and > 70 years (cohort III, elderly). Each cohort was further classified into three groups: (a) control, (b) individuals with cardiovascular risk factors, and (c) those who had a previous cardiovascular event. Novel urinary metabolites linked to cardiovascular risk were identified by nuclear magnetic resonance in cohort I and then evaluated by target mass spectrometry quantification in all cohorts. A previously identified metabolic fingerprint associated with atherosclerosis was also analyzed and its potential risk estimation investigated in the three aged cohorts. Three different metabolic signatures were identified according to age: 2-hydroxybutyrate, gamma-aminobutyric acid, hypoxanthine, guanidoacetate, oxaloacetate, and serine in young adults; citrate, cyclohexanol, glutamine, lysine, pantothenate, pipecolate, threonine, and tyramine shared by middle-aged and elderly adults; and trimethylamine N-oxide and glucuronate associated with cardiovascular risk in all three cohorts. The urinary metabolome contains a metabolic signature of cardiovascular risk that differs across age groups. These signatures might serve to complement existing algorithms and improve the accuracy of cardiovascular risk prediction for personalized prevention. Key messages • Cardiovascular risk in the young and elderly is underestimated. • The urinary metabolome reflects cardiovascular risk across all age groups. • Six metabolites constitute a metabolic signature of cardiovascular risk in young adults. • Middle-aged and elderly adults share a cardiovascular risk metabolic signature. • TMAO and glucuronate levels reflect cardiovascular risk across all age groups.

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Section: Discussionmentioning

confidence: 99%

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

et al. 2020

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“…3-Methyldioxyindole is an oxidation product of 3-methylindole in vivo which is a metabolic product of tryptophan, and produced by bacteria in the colon. In the urine of a patient with acute coronary syndrome, 3-methyldioxyindole was found to be up-regulated as a potential metabolic biomarker ( Wang et al., 2018 ). Zhao et al reported that an obvious decrease of 3-methyldioxyindole was observed in chronic kidney disease rats ( Zhao et al., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Urine Metabolomics Study on Potential Hepatoxic Biomarkers Identification in Rats Induced by Aurantio-Obtusin

Wang²,

Tang³

et al. 2020

Front. Pharmacol.

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Previous studies revealed the hepatotoxic effect of aurantio-obtusin on rats. The aim of this study was to identify potential biomarkers of urine caused by aurantio-obtusin. Sprague–Dawley (SD) rats with body weight of 0, 4, 40, and 200 mg/kg were orally given aurantio-obtusin for 28 days, and urine was collected for 24 h after the last administration. The urine metabolites in the aurantio-obtusin group and the control group were analyzed by ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). Twenty-three metabolites were identified as potential biomarkers, and 10 of them were up-regulated, including xanthosine, hippuric acid, 5-L-glutamyl-taurine, etc. The other 13 biomarkers were down-regulated, including thymidine, 3-methyldioxyindole, cholic acid, etc. The significant changes of these biomarkers indicated that purine metabolism, taurine and hypotaurine metabolism, primary bile acid biosynthesis, pyrimidine metabolism, and tryptophan metabolism played an important role in the hepatotoxicity of aurantio-obtusin in rats. In this paper, the safety and potential risk of aurantio-obtusin were studied for the first time by combining the toxicity of aurantio-obtusin with the results of urine metabolomics, which provided information for the mechanism of liver injury induced by aurantio-obtusin.

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“…Fatty acids are the main source of energy for the heart, accounting for 40–80% of the heart's energy source ( 21 ). A previous study has demonstrated that elevated free fatty acids levels are simply a biomarker of poor overall health, associated with an increased risk of cardiovascular ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

“…in recent years, metabolomics has made progress in cardiovascular research. Wang et al (21) used ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (uPlc-Q-ToF/MS) to conduct urine metabolomics on patients with acute coronary syndrome. The results revealed that nine biomarkers, such as isobutyryl-1-carnitine, were significantly upregulated, and that the expression of 11 biomarkers, including l-lactic acid, were downregulated; in addition, fatty acid metabolism, fatty acid β-oxidation metabolism, amino acid metabolism and the tricarboxylic acid (Tca) cycle were demonstrated to serve important roles in acute coronary syndrome.…”

Section: Introductionmentioning

confidence: 99%

Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry

Liu

Zhang

et al. 2020

Mol Med Rep

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unstable angina (ua) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non-invasive techniques to identify urine biomarkers in patients with ua, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolomics, urine samples from 28 patients with ua and 28 healthy controls (Hcs) were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (uPlc-Q-ToF/MS). a total of 16 significant biomarkers that could distinguish between patients with ua and Hcs, including d-glucuronic acid, creatinine, succinic acid and n-acetylneuraminic acid, were identified. The major metabolic pathways associated with ua were subsequently analyzed by non-targeted metabolomics. The results demonstrated that amino acid and energy metabolism, fatty acid metabolism, purine metabolism and steroid hormone biosynthetic metabolism may serve important roles in ua. The results of the current study may provide a theoretical basis for the early diagnosis of ua and novel treatment strategies for clinicians. The trial was registered with the chinese clinical Trial registration Center (registration no. ChiCTR-ROC-17013957) at Tianjin university of Traditional chinese Medicine.

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Urinary metabonomic study of patients with acute coronary syndrome using UPLC-QTOF/MS

Cited by 23 publications

References 41 publications

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

Urine Metabolomics Study on Potential Hepatoxic Biomarkers Identification in Rats Induced by Aurantio-Obtusin

Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry

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