Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers

Pimpão, Rui C.; Dew, Tristan P.; Figueira, Maria Eduardo; McDougall, Gordon J.; Stewart, Derek; Ferreira, Ricardo B.; Santos, Cláudia N.; Williamson, Gary

doi:10.1002/mnfr.201300822

Cited by 74 publications

(82 citation statements)

References 37 publications

(51 reference statements)

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“…However, the determination of this inter-individual variability is often underreported and most works present the data as average or mean and use the standard error of the mean as a dispersion parameter. Data concerning inter-individual variability after (poly)phenol intake has somewhat been buried in the published scientific literature in the form of plasma concentration ranges [31,33] or box-and-plot whisker graphs [39,40], which give a more accurate picture of the actual variation in a dataset but very few papers have described the CV for each kinetic parameter after (poly)phenol intake. We recently reported that the CV of the AUC of the sum of epicatechin metabolites was 38%, which was comparable to the CV of acetaminophen, consumed by the same individuals [35].…”

Section: Discussionmentioning

confidence: 99%

Bioavailability of wild blueberry (poly)phenols at different levels of intake

Rodriguez-Mateos

Feliciano

Cifuentes-Gomez

et al. 2016

JBR

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Abstract. BACKGROUND:Data from human intervention studies have highlighted potential cardiovascular benefits of blueberry (poly)phenols. However, such biological effects are dependent on their bioavailability and, as such, information on the absorption, metabolism and excretion of such compounds is necessary. OBJECTIVE: To investigate whether the bioavailability of blueberry (poly)phenols is intake-dependent, a group of nine healthy volunteers consumed three wild blueberry drinks containing 766 mg (lower), 1278 mg (medium) and 1791 mg (higher) total (poly)phenols, corresponding to 34, 56 and 80 g, respectively, freeze-dried blueberry powder. METHODS: Plasma levels of (poly)phenol metabolites were assessed at baseline and at 1, 2, 4 and 6 hours post-consumption, using UPLC-Q-TOF mass spectrometry. RESULTS: Twenty-three phenolic acid metabolites were quantified in plasma after blueberry consumption. Increases in plasma (poly)phenol metabolites were observed in all the interventions tested. The area under the curve of the concentration over time (AUC) significantly increased when comparing the lower and higher (poly)phenol interventions. Linear doseresponse regressions were obtained for 11 metabolites, while the plasma concentration of the remaining 12 metabolites was not affected by increasing amounts of (poly)phenols in the blueberry interventions. CONCLUSION: Absorption and metabolism of blueberry (poly)phenols are not exclusively intake-dependent at the amounts tested, evidencing a complex metabolic fate of these compounds.

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Section: Discussionmentioning

confidence: 99%

Bioavailability of wild blueberry (poly)phenols at different levels of intake

Rodriguez-Mateos

Feliciano

Cifuentes-Gomez

et al. 2016

JBR

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“…Note that the y axes are different scales. [87] and [86]. *, p < 0.05; **, p < 0.01 relative to time zero.…”

Section: And 5)mentioning

confidence: 99%

“…2 and 5). Human studies in vivo and gut microbiota-fermentation studies have demonstrated that at least some of these C 6 -C 1 and possibly C 6 -C 2 phenolic acids can be decarboxylated yielding the corresponding phenols (C 6 ) or methyl-phenols (C 6 -C 1 ) [84,[86][87][88][89][90], respectively. Phloroglucinol can also be formed from chalcones and dihydrochalcones [91,92], and at least certain rumen microorganisms can reduce this to non-aromatic dihydrophloroglucinol [93,94].…”

mentioning

confidence: 99%

Role of the small intestine, colon and microbiota in determining the metabolic fate of polyphenols

Williamson

Clifford

2017

Biochemical Pharmacology

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Please cite this article as: G. Williamson, M.N. Clifford, Role of the small intestine, colon and microbiota in determining the metabolic fate of polyphenols, Biochemical Pharmacology (2017), doi: http://dx.doi.org/10.1016/ j. bcp.2017.03.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1Role of the small intestine, colon and microbiota in determining the metabolic fate of in the small intestine, or reaches the colon and is subject to extensive catabolism by colonic microbiota. Untransformed substrates may be absorbed, appearing in plasma primarily as methylated, sulfated and glucuronidated derivatives, with in some cases the unchanged substrate. Many of the catabolites are well absorbed from the colon and appear in the plasma either similarly conjugated, or as glycine conjugates, or in some cases unchanged.Although many (poly)phenol catabolites have been identified in human plasma and / or urine, the pathways from substrate to final catabolite, and the species of bacteria and enzymes involved, are still scarcely reported. While it is clear that the composition of the human gut microbiota can be modulated in vivo by supplementation with some (poly)phenol-rich commodities, such modulation is definitely not an inevitable consequence of supplementation, it depends on the treatment, length of time and on the individual metabotype, and it is not clear whether the modulation is sustained when supplementation ceases. Some catabolites have been recorded in plasma of volunteers at concentrations similar to those shown to be effective in in vitro studies suggesting that some benefit may be achieved in vivo by diets yielding such catabolites. and are also present at high levels in supplements [4], and form essential components of many Chinese medicines [5]. A study within the European Prospective Investigation intoCancer and Nutrition (EPIC) using the Phenol Explorer database reported that the cohort studied consumed 427 different polyphenols including 94 that were consumed at a rate of >1 g/day. The estimated total polyphenol consumption ranged from 584 mg/day by Greek women to 1786 mg/day for men in Aarhus-Denmark. The corresponding data for Greek men and for Aarhus women were 744 mg/day and 1626 mg/ day, respectively, with all data adjusted for age and weighted by season and weekday of dietary recall [6]. This study defined the major contributors to be the phenolic acids (predominantly the caffeoylquinic acids (27-53%), also called chlorogenic acids) and flavonoids (predominantly flavanols (16-29%), proanthocyanidins (5-9%) and theaflavins (14-25%)) [6]. Hydroxybenzoic acids (3.3-7.4%), alkyl-phenols (1.6...

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“…A confirmação da identificação dos metabolitos foi efetuada por LC-MS/MS através da comparação com compostos padrão sintetizados química ou enzimaticamente. Vários metabolitos foram identificados nas amostras de urina, maioritariamente na forma conjugada com grupos metilo, ácido glucurónico e sulfato, sendo que alguns foram identificados em amostras humanas pela primeira vez (PIMPÃO et al, 2014).…”

Section: Metabolitos De Frutas Vermelhas Para Um Envelhecimento Saudáunclassified

Metabolitos de frutas vermelhas para um envelhecimento saudável do cérebro

Santos¹,

Costa²,

Ferreira³

et al. 2015

RBCEH

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ResumoO envelhecimento é considerado um processo natural de ir ficando mais velho e um reconhecido factor de risco para o desenvolvimento de doenças crónicas incluindo as doenças neurodegenerativas. A nutrição têm vindo a ser indicada como um factor importante e modificável com impacto na sáude e bem estar dos idosos. Entre os compostos bioativos da dieta destacam-se os polifenóis, que estão presents em grandes quantidades nos pequenos frutos. A procura de um envelhecimento saudável levou a uma investigação extensiva das propriedades benéficas para a saúde dos polifenóis de pequenos frutos por estudos in vitro, baseados em modelos celulares, modelos animais assim como estudos em humanos. Esta revisão discute o estado-da-arte do conhecimento sobre o impacto dos polifenóis de frutas vermelhas na saúde humana, as lacunas e a estratégia do laboratório de investigação Nutrição Molecular e Saúde para suportar a exploração dos polifenóis de pequenos frutos como moduladores de um envelhecimento saudável.Palavras-chave: Envelhecimento Humano. Nutrição. Polifenóis. Cérebro.

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Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers

Cited by 74 publications

References 37 publications

Bioavailability of wild blueberry (poly)phenols at different levels of intake

Bioavailability of wild blueberry (poly)phenols at different levels of intake

Role of the small intestine, colon and microbiota in determining the metabolic fate of polyphenols

Metabolitos de frutas vermelhas para um envelhecimento saudável do cérebro

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