Urinary level of 1,N6 ‐ethenodeoxyadenosine, a marker of oxidative stress, is associated with salt excretion and ω6‐polyunsaturated fatty acid intake in postmenopausal Japanese women

Hanaoka, Tomoyuki; Nair, Jagadeesan; Takahashi, Yoshiko; Sasaki, Satoshi; Bartsch, Helmut; Tsugane, Shoichiro

doi:10.1002/ijc.10437

Cited by 34 publications

(28 citation statements)

References 15 publications

(20 reference statements)

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“…The corresponding levels of urinary excretion rates in the six subjects were in the ranges 14.2-117.3 pmol Ade/24 h, 53.2-117.8 pmol dC/24 h, and 9.8 -23.4 pmol dA/24 h. The basal levels here are comparable with those of dA [7,16] and dC [11] found previously. Slightly higher excretion levels of Ade than our finding have been reported by Chen and coworkers [9,12].…”

Section: Resultssupporting

confidence: 87%

“…This corresponded to urine concentrations of 5.6 pM, 8.0 pM, and 1.4 pM for Ade, dC, and dA, respectively. The LOQ for Ade was five times lower than reported by Chen and Chang [12], 10 times lower for dC [11] and dA [7], analyzing comparable urine volumes. Figure 2 presents representative chromatograms obtained for a urine sample.…”

Section: Resultscontrasting

confidence: 55%

“…The presence of many interfering substances in concentrations far greater than those of the DNA adducts demands high selectivity and low limit of quantitation for unequivocal identification and quantification. Methods using high-performance liquid chromatography (HPLC) with fluorescence detection have been used for dA determination [4,7] after immunoaffinity clean-up and a very tedious sample preparation. Furthermore, the internal standard was measured separately.…”

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confidence: 99%

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Quantification of urinary etheno-DNA adducts by column-switching LC/APCI-MS/MS

Hillestrøm

Weimann

Poulsen

2006

J. Am. Soc. Mass Spectrom.

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Lipid peroxidation induced etheno-DNA adducts are promutagenic and have been suggested to play a causal role in the development of human cancers. Therefore, human biomonitoring of etheno-DNA adducts in urine has been suggested as a potential marker for oxidative stress-related DNA damage. For quantitative determination, a column-switching LC/APCI-MS/MS method was developed for simultaneous analysis of Ade, dC, and dA in human urine. Quantitative validation parameters (precision, within-day repeatability, and betweenday reproducibility) yielded satisfactory results below 10%. Limit of quantification for Ade, dC, and dA was 5.3 fmol, 7.5 fmol, and 1.3 fmol on column, respectively. Mean urinary excretion rates of a six healthy volunteers were 45.8 pmol Ade/24 h, 96.8 pmol dC/24 h, and 18.1 pmol dA/24 h. The demonstrated levels of performance suggest a future applicability of this method to studies of cancer and other diseases related to oxidative stress in humans. To our knowledge, this is the first method described that allows simultaneous determination of Ade, dC, and dA in human urine samples. [2], are also generated endogenously by reactions of DNA with products derived from lipid peroxidation and oxidative stress [3]. These DNA adducts have miscoding potential and specific repair pathways supporting the hypothesis that -DNA adducts play a causal relationship in carcinogenesis [4]. Furthermore, several studies have found elevated -DNA adduct levels in disorders known as risk factors for cancers [5,6]. Therefore -DNA adducts have been proposed as biomarkers for human cancers associated with certain lifestyles or chronic inflammations [4]. Upon DNA repair, -DNA adducts are excreted in the urine and could, in that respect, be used to investigate the body burden of DNA damage.The analytical procedures for urinary DNA adducts are challenging indeed. The presence of many interfering substances in concentrations far greater than those of the DNA adducts demands high selectivity and low limit of quantitation for unequivocal identification and quantification. Methods using high-performance liquid chromatography (HPLC) with fluorescence detection have been used for dA determination [4,7] after immunoaffinity clean-up and a very tedious sample preparation. Furthermore, the internal standard was measured separately. Several etheno-DNA adducts have been determined in urine from healthy humans using gas chromatography-electron capture negative chemical ionization mass spectrometry (GC/EC-NCI/ MS) [8 -11]. Gas chromatography offers the advantage of greater peak resolution compared to conventional LC. However, the requirement for derivatization before analysis is a major limitation. Recently, LC-MS have been employed in determination of etheno adducts from human urine using electrospray ionization (ESI) [12][13][14]. Atmospheric pressure chemical ionization (APCI) has been used for analysis of dA and 1,N 2 -dG in vitro [15] and of dA in human urine [16].In this paper, we report that solid-phase extraction (SPE) for preconcent...

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Section: Resultssupporting

confidence: 87%

Section: Resultscontrasting

confidence: 55%

mentioning

confidence: 99%

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Quantification of urinary etheno-DNA adducts by column-switching LC/APCI-MS/MS

Hillestrøm

Weimann

Poulsen

2006

J. Am. Soc. Mass Spectrom.

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“…An HPLC fluorescence detection method has been developed for qdA (50) and applied in several studies. For example, in Japanese women, urinary qdA levels were positively associated with increased N-6 polyunsaturated fatty acid intake and NaCl excretion, providing evidence of salt-induced inflammation and lipid peroxidation (51). It has also been reported that short-term fasting does not affect urinary qdA adduct or, indeed, 8-oxodG levels in healthy Korean women despite a reduction in primary lipid peroxidation products (e.g., urinary MDA and 8-isoprostaglandin (57) and have been applied to show a significant association between etheno-DNA adduct excretion rate and the dietary intake of linoleic acid in healthy men (58).…”

Section: Methods Of Analysismentioning

confidence: 99%

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Cooke

Oliński²,

Loft³

2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

205

147

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Background: Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. The noninvasive assessment of such damage, i.e., in urine, and application to large-scale human studies are vital to understanding this role and devising intervention strategies. Methods: We have reviewed the literature to establish the status quo with regard to the methods and meaning of measuring DNA oxidation products in urine. Results: Most of the literature focus upon 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), and whereas a large number of these reports concern clinical conditions, there remains (a) lack of consensus between methods, (b) possible contribution from diet and/or cell death, (c) no definitive DNA repair source of urinary 2 ¶-deoxyribonucleoside lesions, and (d) no reference ranges for healthy or diseased individuals. Conclusions: The origin of 8-oxodG is not identified; however, recent cell culture studies suggest that the

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“…Some animal studies have shown that n-6-enriched diets result in decreased atherosclerosis (George et al, 2000) and several epidemiological studies have found that linoleic acid in the adipose tissue is inversely related to the risk of coronary heart disease (Wood et al, 1987;Tavendale et al, 1992). However, other reports indicate that n-6 fatty acids may decrease HDL concentration (Hodson et al, 2001), increase oxidative stress (Hanaoka et al, 2002) and thus potentially enhance atherosclerosis.…”

Section: Introductionmentioning

confidence: 99%

Dietary intake of n-3, n-6 fatty acids and fish: Relationship with hostility in young adults—the CARDIA study

et al. 2003

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Background: Hostility has been shown to predict both the development and manifestation of coronary disease. Examining the inter-relation of dietary intake of fish and of polyunsaturated (n-3 and n-6) essential fatty acids with hostility may provide additional insights into the cardioprotective effect of dietary fish and polyunsaturated fatty acids. Objective: To examine the association of dietary n-3, n-6 fatty acids and fish with level of hostility in a sample of 3581 urban white and black young adults. Design: Cross-sectional observational study as part of an ongoing cohort study. A dietary assessment in 1992-1993 and measurement of hostility and other covariates in 1990-1991 were used in the analysis. Results: The multivariate odds ratios of scoring in the upper quartile of hostility (adjusting for age, sex, race, field center, educational attainment, marital status, body mass index, smoking, alcohol consumption and physical activity) associated with one standard deviation increase in docosahexaenoic acid (DHA, 22:6) intake was 0.90 (95% CI ¼ 0.82-0.98; P ¼ 0.02). Consumption of any fish rich in n-3 fatty acids, compared to no consumption, was also independently associated with lower odds of high hostility (OR ¼ 0.82; 95% CI ¼ 0.69-0.97; P ¼ 0.02). Conclusions: These results suggest that high dietary intake of DHA and consumption of fish rich in n-3 fatty acids may be related to lower likelihood of high hostility in young adulthood. The association between dietary n-3 fatty acids and hostile personality merits further research.

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Urinary level of 1,N⁶ ‐ethenodeoxyadenosine, a marker of oxidative stress, is associated with salt excretion and ω6‐polyunsaturated fatty acid intake in postmenopausal Japanese women

Cited by 34 publications

References 15 publications

Quantification of urinary etheno-DNA adducts by column-switching LC/APCI-MS/MS

Quantification of urinary etheno-DNA adducts by column-switching LC/APCI-MS/MS

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Dietary intake of n-3, n-6 fatty acids and fish: Relationship with hostility in young adults—the CARDIA study

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