Urinary Leukotriene E4 After Antigen Challenge and in Acute Asthma and Allergic Rhinitis

Taylor, G; Black, Peter; Turner, Nicholas C.; Taylor, Ian; Maltby, NicolaH; Fuller, R W; Dollery, C T

doi:10.1016/s0140-6736(89)91611-5

Cited by 307 publications

(152 citation statements)

References 15 publications

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“…In one study, corticosteroids decreased LT E4 in the EBC of patients with asthma by 18% and had no effect on LT B4 in the EBC 23) ; LT C4 and LT D4 were not measured in that study. The effect of other pharmacological treatments on LTs is mostly unknown, according to the literature data.…”

Section: Discussionmentioning

confidence: 81%

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Leukotrienes B4, C4, D4 and E4 in the Exhaled Breath Condensate (EBC), Blood and Urine in Patients with Pneumoconiosis

Pelclová¹,

Fenclová²,

Vlčková³

et al. 2012

Ind Health

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Leukotrienes (LTs) are involved in the pathogenesis of lung fibrosis and were increased in exhaled breath condensate (EBC) of the patients with pneumoconiosis. However the possible influence of extra-pulmonary disorders on the EBC markers is not known. Therefore in parallel with EBC, LTs' levels in the plasma and urine were measured in patients with pneumoconiosis (45 × asbestos exposure, 37 × silica exposure) and in 27 controls. Individual LTs B4, C4, D4 and E4 were measured by liquid chromatography − electrospray ionization − tandem mass spectrometry (LC-ESI-MS/MS). In EBC, LT D4 and LT E4 were increased in both groups of patients (p<0.001 and p<0.05), comparing with the controls. Both LT B4 and cysteinyl LTs were elevated in asbestosexposed subjects (p<0.05). Asbestosis with more severe radiological signs (s1/s2-t3/u2) and lung functions impairment has shown higher cysteinyl LTs and LT C4 in the EBC (p<0.05) than mild asbestosis (s1/s0-s1/s1). In addition, in the subjects with asbestosis, cysteinyl LTs in EBC correlated with TLC (−0.313, p<0.05) and TLCO/Hb (−0.307, p<0.05), and LT C4 with TLC (−0.358, p<0.05). In pneumoconioses, EBC appears the most useful from the 3 fluids studied.

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Section: Discussionmentioning

confidence: 81%

“…LT E4 is considered to be the final metabolite of LT C4 and LT D4. Consequently, only LT E4 has commonly been analyzed in urine 23,24) .…”

Section: Introductionmentioning

confidence: 99%

Leukotrienes B4, C4, D4 and E4 in the Exhaled Breath Condensate (EBC), Blood and Urine in Patients with Pneumoconiosis

Pelclová¹,

Fenclová²,

Vlčková³

et al. 2012

Ind Health

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“…Leukotrienes B4 and C4 have been detected in the bronchoalveolar lavage fluid of asthmatic subjects (Lam et al, 1988), and urinary excretion of LTE4 increases markedly following allergen challenge and during acute exacerbations of asthma (Taylor et al, 1989). Inhalation of LTE4 by asthmatic subjects results in increased bronchial responsiveness to histamine for up to 1 week (Arm et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Enhanced leukotriene synthesis in leukocytes of atopic and asthmatic subjects.

Sampson

Thomas

Costello

et al. 1992

Brit J Clinical Pharma

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1 We have investigated the capacities of peripheral leukocytes from atopic asthmatic (AA) (n = 7), atopic non-asthmatic (AN) (n = 7), and normal (N) (n = 7) subjects to generate the bronchoconstrictor and proinflammatory mediators leukotrienes (LTs) B4 and C4. 2 Mixed leukocyte preparations containing 61-84% neutrophils, 2.4-15% eosinophils, and 13-29% mononuclear cells were incubated in vitro at 37°C in the presence of calcium ionophore A23187. Synthesis of LTB4 and LTC4 was quantitated by radioimmunoassay.3 Both in dose-response experiments (0-10 J.M A23187 for 5 min), and in time-course investigations (2 jm' A23187 for 0-30 min), the mixed leukocytes of the AA and AN subjects generated on average 4-to 5-fold more LTB4 and 3-to 5-fold more LTC4 than the normal leukocytes (P < 0.01 in all cases; ANOVA). 4 This enhanced LT synthesis by the AN and AA leukocytes was not due to differences in the counts of leukocyte sub-types, or to altered rates of LT catabolism between the subject groups.5 LTB4 synthesis correlated significantly with LTC4 synthesis in the leukocytes of the AN and AA subjects (r = 0.81, n = 14, P < 0.01), but not in those of the normal subjects (r = 0.19, n = 7, P > 0.05). 6 Our results demonstrate an up-regulation of the leukotriene synthetic pathway in the circulating leukocytes of atopic non-asthmatic and atopic asthmatic subjects, which may have important implications in the pathophysiology of asthma and allergy.

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“…This mediator is very unstable and rapidly converted to LTD 4 . Finally LTD 4 may undergo further bioconversion to a less potent metabolite LTE 4 , which is the most stable of the these three compounds and is excreted in the urine [9,10].…”

mentioning

confidence: 99%

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Severien¹,

Artlich²,

Jonas³

et al. 2000

Eur Respir J

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Measurement of leukotriene E 4 (LTE 4 ) in urine is a noninvasive method for assessing changes in the rate of total body cysteinyl leukotriene production. Eosinophil protein X (EPX) has been used to assess eosinophil activity and monitor inflammation in bronchial asthma. The aim of the study was to look for differences in urinary LTE 4 and EPX concentrations between children with stable atopic asthma and healthy controls and to compare asthmatic children with different disease severity. In addition the relationship was evaluated between urinary LTE 4 amd EPX levels and lung function.LTE 4 was also measured (enzyme immunoassay) together with EPX (radioimmunoassay) in urine and lung function tests were carried out in children with mild asthma (steroid-naive) (n=49), moderate to severe asthma (using inhaled steroids) (n=31) and healthy control subjects (n=28).Urinary leukotriene E 4 (LTE 4 ) was significantly higher in children with asthma than in controls (median [25±75 percentile] 238.5 (126.5±375.7) SD 191.8 versus 189 (51±253.2) SD 131.7 pg . mg -1 creatinine; p=0.021). Urinary EPX was also significantly increased in asthmatic children compared with controls (85.5 [64±131.5] SD 76.2 versus 48.5 [43.2±90] 112.1 mg . mmol -1 creatinine; p=0.006). There were no differences in urinary LTE 4 and EPX between the group of mild and the group of moderate to severe asthmatic children. There were significant associations between the urinary LTE 4 and intrathoracic gas volume (ITGV), residual volume (RV), forced expiratory volume in one second (FEV1), forced expiratory capacity (FVC) and maximum expiratory flow rate at 25% of vital capacity (MEF25).Urinary EPX was only correlated with maximum expiratory flow rate at 75% of vital capacity (MEF75). Thus measurement of urinary LTE 4 may predict the degree of airflow obstruction in asthmatic children. Urinary LTE 4 and EPX are useful markers of airway inflammation and can be helpful in guiding asthma management. There was no correlation between LTE 4 and EPX levels. Eur Respir J 2000; 16: 588±592.

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Urinary Leukotriene E4 After Antigen Challenge and in Acute Asthma and Allergic Rhinitis

Cited by 307 publications

References 15 publications

Leukotrienes B4, C4, D4 and E4 in the Exhaled Breath Condensate (EBC), Blood and Urine in Patients with Pneumoconiosis

Leukotrienes B4, C4, D4 and E4 in the Exhaled Breath Condensate (EBC), Blood and Urine in Patients with Pneumoconiosis

Enhanced leukotriene synthesis in leukocytes of atopic and asthmatic subjects.

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

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