Urinary Glycosaminoglycans in Different Phases of Balkan Endemic Nephropathy

Juretić, Dubravka; Čvorišćec, Dubravka; Vuković-Holjevac, Ankica; Benković, Jasminka; Lipovac, K; Stavljenić, A

doi:10.1159/000187564

Cited by 2 publications

(3 citation statements)

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“…In the last few years, we have had much experience with the routine use of SDS-PAGE in diagnosing nephrologic patients, and our recent results have shown that this method is suitable for the early diagnosis of BN and eventually for epidemiologic investigation in the endemic areas. Most probably because of the technical and financial advantages, SDS-PAGE will take the place of other methods proposed for the early diagnosis of BN (13,19,(31)(32)(33) although we do not know if this interdependence is valid for tubular damage of other origins. The same correlation should be examined for interstitial nephritis from other causes such as nephrotoxic, metabolic, immunosuppressive, and cytotoxic drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Urinary tract tumors are often associated with BN (16,17). The protein concentration in the urine is lower than 1 g/L (18)(19)(20).…”

mentioning

confidence: 99%

“…This method already has been successfully applied for routine evaluation of proteinuria and may be helpful in understanding the underlying kidney disease (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). The known electrophoretic investigations of BN proteinuria have been carried out after concentration or dilution of the urinary proteins at a maximum protein concentration of 300 mg/L (19,20). However, complete analysis of all the proteins present in the native urine of patients from a chosen region of Bulgaria using ultrathin-layer SDS pore-graduated PAGE and silver staining has not been completed to date.…”

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confidence: 99%

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Importance of Sodium Dodecyl Sulfate Pore‐Graduated Polyacrylamide Gel Electrophoresis in the Differential Diagnostic of Balkan Nephropathy

et al. 1999

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Balkan nephropathy (BN) is an endemic disease, which leads to end-stage renal failure and artificial renal replacement therapy. Pathologically it is characterized by progressive interstitial nephritis in a large population of villages situated in the proximity of a bend of the Danube up to a distance of 100 km from the river in several parts of Bulgaria, Romania, and the former Yugoslavia. The urinary proteins of 19 patients with BN from the region of Vratza, Bulgaria were examined using ultrathin layer sodium dodecyl sulfate (SDS) pore-graduated polyacrylamide gel electrophoresis (PAGE) and silver staining. The documentation of urinary proteins pattern was performed using laser densitometry and consecutive electronic processing for the purpose of characterizing and quantifying protein excretion. Our results show that the proteinuria of BN is predominantly tubular, consisting of low molecular weight species (10-65 kilodaltons). The amount of tubular protein changes with the progression of the disease. SDS-polyacrylamide gel electrophoresis (PAGE) is a diagnostic method for early diagnosis of tubular failure in BN. Using our method of SDS-PAGE, tubular failure can be detected even at a total protein concentration below 0.1 g/L and when the serum creatinine concentration is normal. Additionally, our method of SDS-PAGE supports the differentiation of BN from glomerular disease.

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Section: Discussionmentioning

confidence: 99%

“…Urinary tract tumors are often associated with BN (16,17). The protein concentration in the urine is lower than 1 g/L (18)(19)(20).…”

mentioning

confidence: 99%