Urinary fructose-1,6-bisphosphatase activity as a marker of the damage to the renal proximal tubules in children with idiopathic nephrotic syndrome

Kępka, Alina; Szajda, Sławomir Dariusz; Stypułkowska, Anna; Waszkiewicz, Napoleon; Jankowska, Anna; Chojnowska, Sylwia; Zwierz, Krzysztof

doi:10.1515/cclm.2008.171

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…In addition, FBPase has also been identified as a bio-marker for assessing damage to the proximal renal tubules [58] . Furthermore, it has been determined that gluconeogenesis is upregulated in patients suffering from Type Ⅱ diabetes [59] .…”

Section: Future Directionsmentioning

confidence: 99%

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Alibhoy¹

2011

WJBC

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Glucose deprivation induces the synthesis of pivotal gluconeogenic enzymes such as fructose-1,6-bisphosphatase, malate dehydrogenase, phosphoenolpyruvate carboxykinase and isocitrate lyase in Saccharomyces cerevisiae . However, following glucose replenishment, these gluconeogenic enzymes are inactivated and degraded. Studies have characterized the mechanisms by which these enzymes are inactivated in response to glucose. The site of degradation of these proteins has also been ascertained to be dependent on the duration of starvation. Glucose replenishment of short-term starved cells results in these proteins being degraded in the proteasome. In contrast, addition of glucose to cells starved for a prolonged period results in these proteins being degraded in the vacuole. In the vacuole dependent pathway, these proteins are sequestered in specialized vesicles termed vacuole import and degradation (Vid). These vesicles converge with the endocytic pathway and deliver their cargo to the vacuole for degradation. Recent studies have identified that internalization, as mediated by actin polymerization, is essential for delivery of cargo proteins to the vacuole for degradation. In addition, components of the target of rapamycin complex 1 interact with cargo proteins during glucose starvation. Furthermore, Tor1p dissociates from cargo proteins following glucose replenishment. Future studies will be needed to elaborate on the importance of internalization at the plasma membrane and the subsequent import of cargo proteins into Vid vesicles in the vacuole dependent degradation pathway.

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Section: Future Directionsmentioning

confidence: 99%

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Alibhoy¹

2011

WJBC

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“…It is also considered as the marker of damage for proximal renal tubules (Kepka et al 2008) and involved in experimental therapeutics for bipolar disorder (Gould et al 2004). Its inhibitor managlinat dialanetil has potential treatment of type 2 diabetes (Wang and Tomlinson 2007).…”

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confidence: 99%

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Zheng

Liu

et al. 2011

Parasitol Res

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Clonorchis sinensis is a food-borne zoonotic parasite that resides in bile ducts and causes clonorchiasis, which may result in cholelithiasis, cholecystitis, hepatic fibrosis, and liver tumors. Although total excretory secretory products (ESP) of C. sinensis adults induce hepatic fibrosis in vivo in rats, the causative mechanism is not well understood. To study components of the ESP, C. sinensis culture medium was collected and analyzed using shotgun LC-MS/MS. We identified a total of 110 proteins, including glycometabolic enzymes (such as fructose-1,6-bisphosphatase (FBPase) and enolase), detoxification enzymes (such as glutamate dehydrogenase, dihydrolipoamide dehydrogenase and cathepsin B endopeptidase), and a number of RAB family proteins. To identify a potential causative agent for hepatic fibrosis, we expressed and purified a recombinant FBPase, a 1,041-bp gene product that encodes a 41.7-kDa protein with prototypical FBPase domains and that can form a tetramer with a molecular mass of 166.8 kDa. In addition, we found that FBPase is an antigen present in the ESP and in circulation. Immunofluorescence showed that FBPase localizes to the intestinal cecum and vitellarium in C. sinensis adults. Our results describe the components of the excretory secretory products from C. sinensis adult worms and suggest that FBPase may be an important antigen present in the ESP of C. sinensis and may lay the foundation for additional studies on the development of clonorchiasis-associated hepatic fibrosis.

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“…Accordingly, similar results were also obtained by proteomic analysis in which Fella and his colleagues found that the down-regulated FBP was involved in the response to cellular stress, which may have its potential usefulness as an early predictive biomarker for liver carcinogenicity [28]. In addition, studies have also reported that FBP activity in the urine and serum can be used to evaluate the degree of graft ischemia during human kidney transplantation, and FBP activity in the urine may also be considered as an indicator of damage to the proximal renal tubules [29,30].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis and Identification of Intestinal FBP as a Predictor of Gut Dysfunction During Heatstroke in Mice

Liu

et al. 2012

Journal of Surgical Research

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Urinary fructose-1,6-bisphosphatase activity as a marker of the damage to the renal proximal tubules in children with idiopathic nephrotic syndrome

Abstract: "The overload" of proximal renal tubules by proteins in children with INS releases FBP-1 into urine. FBP-1 activity in urine may therefore be considered as a marker of damage to the proximal renal tubules in children with INS.

Cited by 10 publications

References 18 publications

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Proteomic Analysis and Identification of Intestinal FBP as a Predictor of Gut Dysfunction During Heatstroke in Mice

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