Urinary Exosomal MiRNA-4534 as a Novel Diagnostic Biomarker for Diabetic Kidney Disease

Abstract

Urinary exosomal miRNAs can reflect the physiological and possibly pathophysiological state of cells lining the kidney and participate in the regulation of transcription and translation of proteins, which are playing an important role in the pathogenesis of diabetic kidney disease. In the present study, urine was collected from DM and DKD patients with a duration more than 10 years and urinary exosomal miRNA profiling was conducted in urinary exosomes obtained from three patients with type 2 diabetes (DM) and three patients with type 2 diabetic kidney disease (DKD) using Exiqon's microRNA arrays. In total, the expression of 14 miRNAs (miR-4491, miR-2117, miR-4507, miR-5088-5P, miR-1587, miR-219a-3p, miR-5091, miR-498, miR-4687-3p, miR-516b-5p, miR-4534, miR-1275, miR-5007-3p, and miR-4516) was up-regulated (>2-fold) in DKD patients compared to healthy controls and DM patients. We used qRT-PCR based analysis of these 14 miRNAs in urinary exosomes from 14 DKD to 14 DM patients in confirmation cohort, among which seven miRNAs were consistent with the microarray results. The expressions of miR-4534 and miR-516b-5p correlated with trace proteinuria levels in the confirmation cohort. In conclusion, it has been confirmed that the expression of urinary exosomal miRNA in patients with type 2 diabetes DKD has changed. Mir-4534 might affect the FoxO signaling pathway by targeting BNIP3, and is expected to become a new biomarker for the progression of type 2 DKD disease, which will provide further research on the pathogenesis of DKD.

LncRNA-URHC Functions as ceRNA to Regulate DNAJB9 Expression by Competitively Binding to miR-5007-3p in Hepatocellular Carcinoma

“…In addition, the results revealed that expressed of miR-5007-3p was upreglated in patients with positive lymphatic metastasis of primary gastric tumors [ 36 ]. In addition, another experiment by RT-qPCR indicated that the relative expression level of miR-5007-3p was significantly upregulated in the diabetic kidney disease group compared to type 2 diabetes group [ 37 ]. However, these researches did not examine miR-5007-3p specific cellular functions, and miR-5007-3p expression in HCC is unclear.…”

LncRNA-URHC Functions as a ceRNA to Regulate Danjb9 Expression by Competitively Binding to miR-5007-3p in Hepatocellular Carcinoma

“…In addition, the results revealed that expressed of miR-5007-3p was upreglated in patients with positive lymphatic metastasis of primary gastric tumors [33]. In addition, another experiment by RT-qPCR indicated that the relative expression level of miR-5007-3p was signi cantly upregulation in the Diabetic kidney disease group compared to type 2 diabetes group [34]. However, these researches did not examine miR-5007-3p speci c cellular functions, and miR-5007-3p expression in HCC is unclear.…”

Deciphering the link between Diabetes mellitus and SARS-CoV-2 infection through differential targeting of microRNAs in the human pancreas

“…hsa-miR-4279 is expressed in urinary extracellular vesicles of Diabetic patients with macroalbumin, while hsa-miR-3941 is linked with islet immunity [ 92 – 94 ]. hsa-miR-5088-5p is upregulated in urine in the case of Diabetic Kidney disease [ 95 ]. hsa-miR-4747-5p is downregulated in human aortic vascular smooth muscle cells with high glucose-induced calcification/senescence [ 96 ].…”