Urinary Excretion Rates of Multiple Renal Indicators after Kidney Transplantation: Clinical Significance for Early Graft Outcome

Matteucci, Elena; Carmellini, Mario; Bertoni, C; Boldrini, E; Mosca, Franco; Giampietro, Ottavio

doi:10.3109/08860229809045118

Cited by 27 publications

(17 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the main renal functional changes observed in the acute postoperative phase in aTX-NHE-I in electrolyte excretion, reduced protein reabsorption, and polyuria are predictive of favorable graft outcome in humans [21] (in contrast to long-term observations). A possible counterregulatory influence on ENaC due to upstream NHE-I by enhanced ENaC activity was excluded (Fig.…”

Section: Discussionmentioning

confidence: 97%

Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection

Reuter

Velić

Edemir

et al. 2008

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Acute rejection in renal transplantation disturbs solute and volume maintenance in humans accompanied by delayed graft function and poor prognosis. We recently reported that decreased expression and function of Na+/H+ exchanger type 3 (NHE-3) in proximal tubules and epithelial Na+ channels and aquaporin 2 in collecting ducts are major mechanisms involved in Na+ and water imbalances shortly after transplantation in rat undergoing acute rejection. We performed kidney transplantations in rats with bilaterally nephrectomized recipients with acute rejection and, in addition, systemically administered a specific inhibitor of NHE-3 (NHE-I). NHE inhibition in acute renal failure was shown to improve tubular function and recovery. The aim of this therapy was to reduce energy consumption of the graft and preserve NHE-3 function. Imbalances in electrolyte excretion declined in NHE-I-treated animals and NHE-3 activity was preserved. Observed NHE-I-dependent changes in electrolyte excretion, polyuria, and reduced protein reabsorption in the acute postoperative phase are predictors of favorable graft outcome in humans.

show abstract

Section: Discussionmentioning

confidence: 97%

Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection

Reuter

Velić

Edemir

et al. 2008

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…Early post-transplant high diuresis volume and low proteinuria can reportedly predict graft outcome [18,23]. Although our study was limited to 7 days, we therefore believe that our data provide good evidence that long term kidney graft function in NHB donor grafts can also be positively influenced through hyperthermia preconditioning.…”

Section: Discussionmentioning

confidence: 67%

Hyperthermia-induced HSP expression correlates with improved rat renal isograft viability and survival in kidneys harvested from non-heart-beating donors

Redaelli

Wagner

Kulli

et al. 2001

Transplant International

View full text Add to dashboard Cite

Transient sublethal hyperthermia followed by recovery from heat stress, referred to as heat shock preconditioning, exerts a protective effect on ischemiaheperfusion-induced injury in many systems. This effect is considered to be correlated to heat shock proteins (HSPs) and might be a critical factor in kidney graft function and survival. This study was designed to examine the impact of heat shock preconditioning on kidney isograft function and survival in a model utilizing non-heart-beating (NHB) donors. Four groups of male Lewis rats (n = 10/group) subjected either to whole body hyperthermia (groups A and C) or to sham anesthesia (groups B and D) were allowed 24 h recovery. Thereafter, 20 min of warm ischemia (A/B), and in a separate set of experiments 40 min of warm ischemia (C/D), were induced by suprarenal aortic cross clamping before renal procurement. After 24-h preservation with University of Wisconsin solution at 4 "C, orthotopic kidney transplantations were performed to syngeneic bilaterally nephrectomized recipients. Tissue specimens were taken to determine HO-l/HSP32,72, and 90 induction by Western blot analysis. Renal function was measured by means of serum creatinine and creatinine clearance on days 0,3, and 7 as well as urine volume, protein content, and creatinine levels daily. HO-1/ HSP32 and HSP72 were found to be expressed constitutively. Moreover, heat shock strongly induced renal HSP72 and HSP32/HO-1, and to a lesser extent HSP90, expression. For recipients of group A grafts, the graft survival rate was 10/10, whereas it was 7/10 (70 %) in recipients of group B grafts (log rank p < 0.05). Following 40 min of warm ischemia, 6/10 (60 %) recipients survived, whereas all sham treated animals died with anuria within 6 days (log rank p = 0.01). Heat shock preconditioning strongly improved graft viability and reduced functional impairment. Creatinine clearance (CRC) on day 3 post Tx was 0.43 f 0.24 ml/min in preconditioned animals (group A) and 0.07 f 0.09 ml/min (p < 0.001) in sham preconditioned (group B), whereas it was 0.91 f 0.33 ml/min and 0.03 f 0.02 ml/min (p < 0.00001) on day 7 post Tx. Following 40 min NHB time, CRC in survivors of preconditioned graft recipients (group C) was 0.32 & 0.2 ml/ min (day 3 post Tx) and 0.23 f 0.08 mllmin (day 7 post Tx) and was significantly better than CRC of group B (p < 0.01 and p < 0.00001, respectively). CRCs prior to NHB procedures were comparable in all animals ranging between 1.31 and 1.72 ml/min. Serum creatinine as well as proteinuria were significantly increased after transplantation in both groups but 352 recovered within 5 days in recipients of preconditioned grafts, whereas kidneys from donors without HP did not recover function. Histological alterations were also diminished following HP. Hyperthermic preconditioning induces strong and long lasting HO-UHSP32, HSP72, and HSP90 expression in rat kidKeywords Heat shock protein. neys. HP increases survival followHeme oxygenase-1 * Non-hearting transplantation and improves beating-donor . NHB...

show abstract

“…Moreover, proteinuria was clearly reduced following preconditioning, while urine volume and creatinine excretion were increased. An early high diuresis volume and low proteinuria can reportedly predict graft outcome [30, 31]. Although our study was limited to 7 days, we therefore believe that our data provide good evidence that long-term kidney graft function can also be positively influenced by HP.…”

Section: Discussionmentioning

confidence: 67%

Hyperthermia Preconditioning Induces Renal Heat Shock Protein Expression, Improves Cold Ischemia Tolerance, Kidney Graft Function and Survival in Rats

Redaelli¹,

Tien²,

Kubulus

et al. 2002

Nephron

View full text Add to dashboard Cite

Background: Evidence indicates that hyperthermia preconditioning (HP) can be protective in kidney transplantation, possibly through increased heat shock protein (HSP) expression. A detailed study about individual HSPs and functional preservation is lacking, however. Therefore, we studied the effects of HP on kidney graft survival, function and HSP expression. Methods: Male Lewis rats were or were not subjected to whole-body hyperthermia 24 h prior to kidney procurement. Kidneys were stored in UW solution at 4°C for 32, 40 or 45 h. Recipient kidneys were both removed and single isografts transplanted orthotopically. Results: HP strongly induced HSP72 and HSP32 expression. Following 32-hour cold ischemia, most animals survived even without prior HP. However, HP strongly reduced functional impairment induced by cold ischemia. Following 40-hour cold ischemia, kidneys from donors without HP did not recover function and all animals died within 3 days. In contrast, HP-exposed kidneys tolerated 40-hour storage significantly better, with 44% of rats surviving until sacrifice on day 7. In these animals, renal function was still better compared to animals with 32-hour-stored kidneys without HP. Histological alterations were also diminished following HP. Conclusion: Our data show that HP induces renal HSP72 and, for the first time, HSP32. HP increases survival following transplantation and acts by improving several parameters of kidney function including proteinuria, volume output and creatinine clearance.

show abstract

Urinary Excretion Rates of Multiple Renal Indicators after Kidney Transplantation: Clinical Significance for Early Graft Outcome

Cited by 27 publications

References 5 publications

Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection

Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection

Hyperthermia-induced HSP expression correlates with improved rat renal isograft viability and survival in kidneys harvested from non-heart-beating donors

Hyperthermia Preconditioning Induces Renal Heat Shock Protein Expression, Improves Cold Ischemia Tolerance, Kidney Graft Function and Survival in Rats

Contact Info

Product

Resources

About