URINARY EXCRETION OF Δ 5 -PREGNENETRIOL AND OTHER 3β-HYDROXY-Δ 5 STEROIDS BY SUBJECTS WITH AND WITHOUT ENDOCRINE DISEASE

Interstitial cell carcinoma of the testis is a singularly rare steroid-producing cancer. Of the seven reported cases (1-7), urinary 17-ketosteroid (17-KS) excretion was high in the four cases in which it was measured. Abelson, Bulaschenko, Trommer, and Valdes-Dapena (7) fractionated the urinary 17-ketosteriods and corticoids in one recently reported case. There is, however, no comprehensive study of either the production of androgens or related steroids by this tumor. (testosterone) ; 17a-hydroxy-androst-4-en-3-one (epitestosterone); 3a-hydroxy-5,B-androstan-17-one (etiocholanolone); 3a-hydroxy-5a-androstan-17-one (androsterone); 3a,11,8-dihydroxy-51-androstan-3-one (11-hydroxyetiocholanolone); 3a-hydroxy-58-androstan-3,11-dione (ll-ketoetiocholanolone) ; 3a,11'B-dihydroxy-Sa-androstan-17-one (1l-hydroxy-androsterone); 3,B-hydroxy-androst-5-en-3-one (dehydroepiandrosterone, DHA); androst-4,en-3,17-dione (androstenedione); 3fi,16a-dihydroxy-5-androstan-17-one (16-hydroxy DHA); androst-5-en-3,8,17P-diol (androstenediol); pregn-5-en-3p8,20a-diol (pregnenediol); pregn-5-en-3fi,17a,20a-triol (pregnenetriol) ; 5,6-pregnan3a,20a-diol (pregnanediol); 5P-pregnan-3a,17a,20a-triol (pregnanetriol); 3a,17a-dihydroxy-5p8-pregnan-20-one (17-hydroxypregnanolone); 3a,17a,20a-trihydroxy-59-pregnan-l1-one (11-ketopregnanetriol); 5a-androst-16-en- 3,20-dione (cortisol) ; 3ac,11P,17a,21 -tetrahydroxy-5#9-pregnan-20-one (tetrahydrocortisol); 3a,17a,21-trihydroxy-5fi-pregnan-11,20-dione (tetrahydrocortisone) ; 3a, 11P,17a,21-tetrahydroxy-5a-pregnan-20-one (allotetrahydrocortisol) ; 3a,17a,21 -trihydroxy-5Sf-pregnan-20-one (tetrahydro substance S); 3-hydroxyestratrien-1,3,5 (10)-17-one (estrone); 1,3,5 (10-estratriene-3,17P-diol (estradiol); 1,3,5 (10)-estratriene-3,16a,17P-triol (estriol). 1700

supporting

confidence: 89%

Metabolism of testoterone and related steroids in metastatic interstitial cell carcinoma of the testis.

Lipsett¹,

Sarfaty²,

Wilson³

et al. 1966

“…In the case of dehydroepiandrosterone, however, interposition of the Decalin paper system was necessary to reduce H3 contaminants. In each case the dimethylhydrazones had the same H'/C" ratio as the corresponding Step 1 Washing only after acetylation 1.10 >100 >100 Gas-liquid chromatography 0.36 8.5…”

Section: Comments On Methodsmentioning

confidence: 96%

Plasma Ketosteroids and Testosterone in Man: A Study of the Pituitary-testicular Axis*

Kirschner¹,

Lipsett²,

Collins³

1965

Self Cite

122

The relationship between the Leydig cell of the testis and the tropic hormones of the anterior pituitary gland has been inadequately explored in man because of methodological difficulties. The development of methods for measuring plasma testosterone 1 levels in this and other laboratories has made such studies possible. We have examined the ability of the Leydig cell to respond to stimulation by gonadotropin, its inhibition by exogenous androgen, diurnal variation of plasma testosterone, and the role of the adrenal cortex in the maintenance of plasma testosterone levels in man.To measure plasma testosterone, we have used a double isotope derivative method and employed gas-liquid chromatography as the final chromatographic step. This method has also been applied to the measurement of the plasma levels of dehydroepiandrosterone, androsterone, and etiocholanolone. The methods will be presented in detail, and we shall show that the relationship between the testicular Leydig cell and the anterior pituitary gland is similar to the relationship between the adrenal cortex and the anterior pituitary gland. MethodsThe plasma steroids were measured by a double isotope derivative method using C14-labeled steroids to monitor recovery and H'-acetic anhydride to measure mass.

“…Water was glass-distilled after the addition of a few crystals of KMnO4. n-Hexane, ether, chloroform, and methanol were prepared as previously described (7,8 Silica gel G 4 was washed twice with absolute ethanol and once with redistilled ethanol. After the third wash, the wet powder was heated overnight in an oven at 1000 C and then stored at room temperature in a desic-…”

Section: Methodsmentioning

confidence: 99%

“…Water was glass-distilled after the addition of a few crystals of KMnO4. n-Hexane, ether, chloroform, and methanol were prepared as previously described (7,8). Ligroin 2 was prepared exactly like n-hexane; on redistillation, the fraction boiling from 1030 C to 1060 C was collected.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Testosterone Production Rates in Normal Adults

Korenman¹,

Wilson²,

Lipsett³

1963

Self Cite

The investigation of clinical and physiological problems related to androgen production has been hampered by lack of an adequate measure of testosterone production. As one approach to this problem, Finkelstein, Forchielli, and Dorfman (1) developed a sensitive method for the measurement of free testosterone in plasma. The subsequent identification of testosterone in the urine (2) as the glucuronoside (3) provided a unique metabolite for the estimation of testosterone production rate by the isotope-dilution method. One such study, using an isotope-derivative method to quantitate testosterone, was briefly reported by Hudson, Coghlan, Dulmanis, and Ekkel (4).We have measured urinary testosterone by an adaptation of the fluorescence reaction described by Wilson (5). This has facilitated the use of the isotope-dilution method for the measurement of testosterone production rates in man. MATERIALS AND METHODSL.S., L.M., G.S., R.S., J.S., C.Z., and M.G. were healthy young adult volunteers C.S. was a 27-year-old white woman in complete remission after treatment for metastatic choriocarcinoma. Regular menses had occurred for the 6 months before study. E.H. was a 27-year-o'dNegro woman with normal menstrual function admitted for treatment of local recurrence of carcinoma of the breast.Absolute ethanol 1 was redistilled by the method of Peterson and his associates (6). Water was glass-distilled after the addition of a few crystals of KMnO4. n-Hexane, ether, chloroform, and methanol were prepared as previously described (7,8 Silica gel G 4 was washed twice with absolute ethanol and once with redistilled ethanol. After the third wash, the wet powder was heated overnight in an oven at 1000 C and then stored at room temperature in a desic-cator. An alcohol eluate of a 10-g sample of the powder should give no colored residue.The steroids 5 used were obtained from commercial sources. Testosterone and testosterone acetate were recrystallized, and the melting points agreed with reported values. Other materials used were human folliclestimulating hormone (FSH) (potency, 0.1 ml = 1 U NIH FSH-S1) contaminated with a small amount of luteinizing hormone, human chorionic gonadotropin (HCG) ,6 and testosterone-4-C 7 (77 ,&c per mg), which was chromatographed in systems A2 and B6 before use.Partition column chromatography. The technique previously described (7) was modified so that extracts of 1.5 days' urine could be resolved on a single column.The glass tube was 48 mm i.d. and 30 mm long with a 55: 50 outer joint at the top. Solvent systems are shown in Table I. Seventy g of silica-alumina catalyst, used as supplied, was mixed with 43 ml stationary phase A and packed in about 140 ml mobile phase A previously poured into the column. The dried urine extract was applied with successive portions of 1.2, 0.6, and 0.3 ml stationary phase A, each mixed with an equal volume of mobile phase A. Each transfer was preceded by placing layers of 2, 1, and 0.5 g dry silicate on the column. To develop the column, the successive solvents were allowed to ...