Urinary Excretion of Androstenedione, Testosterone, Epitestosterone and Dehydroepiandrosterone During the Normal Menstrual Cycle

Abstract

The urinary excretion of total androstenedione i. e. free, glucosiduronide and sulphate, testosterone and dehydroepiandrosterone during two normal menstrual cycle is presented. Epitestosterone was determinated during one cycle only. Both cycles were ovulatory as can be seen from the pregnanediol and oestriol determinations. The androstenedione and testosterone excretion values showed a caracteristic decrease from 12th to 16th day and an increase during the luteal phases, reaching their maximal levels between the 20th and 25th (cycle A) and 20th and 24th day (cycle B) respectively. The excretion values of epitestosterone were highest on 22nd and 23rd day. Dehydroepiandrosterone values were also slightly raised during the luteal phase of the cycle.

Urinary steroid profile in females – the impact of menstrual cycle and emergency contraceptives

“…[4,15] This is partly due to endogenous concentrations of testosterone, epitestosterone and the diols are near the detection limit in females and therefore the results of the corresponding ratios are prone to larger variations. [9] Our pilot study indicates that a single dose of an EC does not influence the T/E ratio to the same extent as continuous use of HC. [16] Androsterone and Etio are both found at 1000-times higher concentrations than the other metabolites and hence the analytical sensitivity is not of any concern and therefore associated with smaller intra-individual variation.…”

“…Altogether the individual variation, here measured as CV, of the ABP biomarkers are larger in women than in men. [8,9] Epitestosterone was the only affected urinary steroid, where an increase on days 14 and 21 was observed. In agreement with another study in men, the A/Etio ratio show the smallest CV.…”

“…[1,2] The ABP steroidal module consists of the urinary concentrations of testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5α-androstan-3α,17β-diol (5α-diol) and 5β-androstan-3α,17β-diol (5β-diol), as well as the T/E, T/A, A/Etio, 5α-diol/5β-diol and 5α-diol/E ratios. [6,7] This is due to hormonal fluctuations throughout the menstrual cycle [8,9] and due to the fact that the concentrations of some metabolites are near the detection limit in female samples. [6,7] This is due to hormonal fluctuations throughout the menstrual cycle [8,9] and due to the fact that the concentrations of some metabolites are near the detection limit in female samples.…”

“…[3] Previous studies have shown that there is a large intra-individual variation in these ratios [4,5] which appears to be even larger in women. [6,7] This is due to hormonal fluctuations throughout the menstrual cycle [8,9] and due to the fact that the concentrations of some metabolites are near the detection limit in female samples.…”

Characterization of 17α-hydroxysteroid dehydrogenase activity (17α-HSD) and its involvement in the biosynthesis of epitestosterone

“…In hyperplasic prostate, epiT concentration is comparable to that of 4-dione, which represents about twice the amount of T and half the concentration of dihydrotestosterone (DHT) [7]. The excretion of epiT in urine is slightly lower than that of T [8-11]. Plasma concentrations of epiT decline with age and were established at approximately 2.5 nmol/l in adult men and 1.2 nmol/l in women.…”