Urinary Excretion of 16α-Hydroxydehydroepiandrosterone and 16-Ketoandrostenediol During the Early Neonatal Period

Cleary, Robert E.; Pion, R.

doi:10.1210/jcem-28-3-372

Cited by 19 publications

(3 citation statements)

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“…Both steroids were measured in large quantities (table VI). Previous studies have already shown this (8,10,(52)(53)(54)56). The same is true for amniotic fluid (1,2).…”

Section: Discussionsupporting

confidence: 64%

Studies on Steroids in Urine of the Male Newborn

Schindler¹,

Wuchter²

1975

Neonatology

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Four different methods of isolation and purification were utilized to study steroids in urine of male newborns which was collected during the first 5 days of life. These methods included celite column, ion exchange column and thin-layer chromatography, solvolysis and enzyme hydrolysis with β-glucuronidase and aryl sulfatase. Procedural losses were evaluated by using radioactive internal standards. Final quantitation of each steroid was achieved by comparison of its chromatographic and quantitative behavior with the respective standard steroids on various gas-liquid chromatography systems, either as parent compound or as trimethylsilyl ether derivative. The following steroids were found in the amounts indicated: progesterone, 2.1 μg/l (pool I), 4.6 μg/l (pool III); pregnanediol, 625.0 μg/l (pool IIa), 605.0 μg/l (pool IIb glucuronide), 25.4 μg/l (pool IIb sulfate), 4.2 μg/l (pool IIb free), 729.0 μg/l (pool III); 16α-hydroxyprogesterone, 713.0 μg/l (pool III); 16α-hydroxypregnenolone, 14,000.0 μg/l (pool III); 16α-hydroxydehydro-epiandrosterone, 2,350.0 μg/l (pool III); 16-dehydroprogesterone, 155.0 μg/l (pool I), 21.2 μg/l (pool IIb glucuronide), 97.5 μg/l (pool IIb sulfate), 5.3 μg/l (pool III); 16-de-hydropregnenolone, 382.0 μg/l (pool I), 1,380 μg/l (pool IIb glucuronide), 172.0 μg/l (pool IIb sulfate), 174.0 μg/l (pool III); 16-dehydropregnanolone, 8.3 μg/l (pool I), 239.0 μg/l (pool IIb sulfate). Pregnenolone, pregnanolone, 17α-hydroxyprogesterone and 17α-hydroxypregnenolone could not be detected. The results support the concept that the steroid patterns of urine of the newborn and amniotic fluid are very similar and that the amniotic fluid steroid content is mainly dependent on fetal urinary steroid excretion. The data on Δ¹⁶-C₂₁ -steroids are discussed.

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“…Both steroids were measured in large quantities (table VI). Previous studies have already shown this (8,10,(52)(53)(54)56). The same is true for amniotic fluid (1,2).…”

Section: Discussionsupporting

confidence: 64%

Studies on Steroids in Urine of the Male Newborn

Schindler¹,

Wuchter²

1975

Neonatology

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“…Hyperfunction of the fetal adrenals [1,33,34,35] would increase estradiol, only if associated with a relative lack of the 16 a hydroxylating activity of the fetal liver. A deficit of 16 a hydroxylated compounds was found in the urine of one newborn of a diabetic mother [36]. The low pregnandiol excretion [1] in the presence of normal or high maternal plasma progesterone in diabetic pregnancy [37,38] might also result from fetal liver insufficiency [1].…”

Section: Discussionmentioning

confidence: 99%

Plasma concentrations of unconjugated estrone, estradiol-17β and estriol, and HCS throughout pregnancy in diabetics and gestational diabetics

Hertogh¹,

Thomas²,

Hoet³

et al. 1976

Diabetologia

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Summary. Plasma unconjugated estrone (E~), estradiol-17/3 (E2) and estriol (E3) , and HCS were measured in the same plasma samples collected throughout pregnancy in 19 gestational diabetics (GD) and 21 diabetics (D). When compared to the results obtained in 22 normal subjects, plasma levels of E 1 and E 2 were significantly elevated in D in the second half of gestation. The results were intermediate although closer to the normals, in GD. E 3 values were not different from the normals in both D and GD. HCS values were lower than normal in early pregnancy in both D and GD. In late pregnancy HCS levels were not different from normal in either D or GD, although some individual values were much above the upper limit in some diabetic patients. The hormonal ratios in D and GD parallel those in normals, although E3/E 2 and HCS/E 2 were lower in D. These results are discussed with respect to the different behaviour of E 2 and E3, taking into accotmt the difference in their respective biosynthetic pathways. Besides a possible quantitative modification of the placental function in D, the results could tentatively be explained by a qualitative change in the fetal estrogen precursors to placental aromatization, in favour of the 16-non-hydroxylated compound. However, maternal modifications in precursor production or in estrogen metabolism can be an alternative hypothesis. Finally, the present work does not support the hypothetical estrogen deficiency in diabetic pregnancy. Estrogen treatment appears to have no objective justification.* This work was presented at the 1 lth annual meeting of the E. A.S.D., Munich September 1975 (Abstract 46, Diabetologia 11, 337, 1975.Key words: Estrone, estradiol, estriol, estrogens, diabetes, pregnancy, HCS, gestational diabetes, plasma levels.The hormonal environment in diabetic pregnancy has been the subject of many investigations, which have been extensively reviewed recently [1,2]. These works were aimed at showing the possible occurrence of endocrine dysfunction of the placenta, induced by the maternal diabetic environment, or of the fetus where adaptative mechanisms might have been stimulated by the particular metabolic situation. Yet, estrogen administration has been advocated as a necessary part of the treatment in diabetic pregnancy [3], in view of inferred hormonal insufficiency based on urine analysis [1,4].Controversy has arisen concerning the significance of urinary measurements [5], and conflicting results were reported [6]. Plasma measurements of estrogens also gave inconclusive results depending on the technique used, or the selection of patients [1,4,7], regarding for instance their state of equilibrium or the degree of illness. Low [8], high [9][10], and normal [11][12][13][14][15] values were reported for plasma estrogens. High [16,17] and normal [18,19] values were found for plasma HCS (human chorionic somatomammotropin).In the present study, unconjugated estrogens (estrone, estradiol-17/3 and estriol) and HCS were measured in the same plasma samples of a group of well tr...

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“…eclampsia, reduced P levels would also have been expected, provided that the metabolism of P was unaffected in pre-eclampsia. In that case, other factors such as a reduced supply of fetal oestrogen precursors and/or a reduced placental aromatase activity may be main reasons for the decreased oestrogen produc tion [Cleary and Pion, 1968;Lehmann et al" 1973;Rahman et al, 1975], However, the lack of difference in P levels may also reflect an impaired hepatic P metabolism in pre eclamptic subjects, which may conceal a vir tual decrease in placental P production (vide infra). The different patterns for free and conju gated oestrone and oestriol and the signifi cantly higher E|/tE| and E3/tE3 ratios in pre eclampsia, indicate an altered maternal oes trogen metabolism.…”

Section: Discussionmentioning

confidence: 99%

Serum Levels of Unconjugated and Total Oestrogens and Dehydroepiandrosterone, Progesterone and Urinary Oestriol Excretion in Pre-Eclampsia

Rosing

Carlström²

1984

Gynecol Obstet Invest

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Peripheral serum levels of unconjugated (E₁ and total (tE₁) oestrone, unconjugated oestradiol-17Β, unconjugated (E₃) and total (tE₃) oestriol, progesterone, unconjugated dehydroepiandrosterone (DHA) and dehydroepiandrosterone sulphate (DHAS), and urinary tE₃ excretion were determined in pre-eclamptic patients at gestational weeks 32 and 36 and in women with normal pregnancy at the corresponding length of gestation. DHA and DHAS were also analyzed in samples taken 6 weeks after delivery. There were no differences between pre-eclamptic and normal subjects in unconjugated oestrogens and progesterone. Serum tE₁ and tE₃ levels and urinary tE₃ were significantly lower, and the E₁/tE₁ and E₃/tE₃ ratios significantly higher in the pre-eclampsia patients. Serum DHA and the DHA/DHAS ratio were significantly elevated in the pre-eclampsia patients during pregnancy, while no differences in these respects were found in the samples taken 6 weeks after delivery. There were no differences in serum DHAS levels. The findings are thought to reflect a general decrease in fetoplacental oestrogen production in combination with a reduced hepatic steroid conjugation in pre-eclamptic subjects.

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Urinary Excretion of 16α-Hydroxydehydroepiandrosterone and 16-Ketoandrostenediol During the Early Neonatal Period

Cited by 19 publications

References 0 publications

Studies on Steroids in Urine of the Male Newborn

Studies on Steroids in Urine of the Male Newborn

Plasma concentrations of unconjugated estrone, estradiol-17β and estriol, and HCS throughout pregnancy in diabetics and gestational diabetics

Serum Levels of Unconjugated and Total Oestrogens and Dehydroepiandrosterone, Progesterone and Urinary Oestriol Excretion in Pre-Eclampsia

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