Urinary Enzyme Excretion and Changes in Renal Functions Induced by Toxic Substances or by Renal Ischemia in Rats

Ansermet, François; Miéville, C; Diezi, J.

doi:10.1007/978-3-642-67729-8_43

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…Medina et al found no difference in the degree of renal failure analyziing 12 serum urea levels at shortterm kidneys IR between IGF-I50 μg/100 g reperfusion treatment than untreated controls male rats. Ansermet et al 13 found plasma urea levels not depressed after the higher doses of toxic agents measured within 48 h after either mercuric chloride, or gentamicin, or tobramycin administration or renal arteries IR in adult male Wistar rats. Lin et al…”

Section: Discussionmentioning

confidence: 96%

The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

Tsompos

Panoulis

Toutouzas³

et al. 2017

Ital J Med

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This experimental study examined the effect of the antioxidant drug U-74389G, on a rat model and particularly in a renal ischemia-reperfusion protocol. The effects of that molecule were studied biochemically using blood mean urea levels. Forty rats of mean weight 231.875 g were used in the study. Urea levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. U-74389G administration significantly decreased the predicted urea levels by 11.35%±2.73% (P=0.0001). Reperfusion time non-significantly increased the predicted urea levels by 2.26%±3.29% (P=0.4103). However, U-74389G administration and reperfusion time together significantly decreased the predicted urea levels by 6.31%±1.70% (P=0.0005). U-74389G administration whether it interacted or not with reperfusion time had significant decreasing effect on the urea serum levels, reflecting a respective renal function augmentation.

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Section: Discussionmentioning

confidence: 96%

The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

Tsompos

Panoulis

Toutouzas³

et al. 2017

Ital J Med

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“…Thus we cannot exclude that the increased renal tubular enzyme excretion following amphotericin B application results from a reduction of renal blood flow. However we consider this possibility unlikely as in vivo and in vitro as well amphotericin B has been shown to increase renal tubular permeability 13,17 . In addition it has been demonstrated that amphotericin B inhibits accumulation of p ‐aminohippuric acid in rat renal cortical slices 8 …”

Section: Discussionmentioning

confidence: 97%

“…However we consider this possibility unlikely as in vivo and in vitro as well amphotericin B has been shown to increase renal tubular permeability. 13,17 In addition it has been demonstrated that amphotericin B inhibits accumulation of p-aminohippuric acid in rat renal cortical slices. 8 Independent of the underlying mechanism, the results of the present study suggest that amphotericin B induces damage to the proximal renal tubuli soon after application of the drug which persists at least 4 days with a concomitant decrease in the creatinine clearance.…”

Section: Discussionmentioning

confidence: 99%

Enzymuria following amphotericin B application in the rat

Inselmann

Balaschke

Heidemann

2003

Mycoses

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The effect of amphotericin B application on urinary renal tubule enzyme excretion was investigated in rats treated with amphotericin B (1.5 mg kg-1 b.i.d., i.v.) for 4 days. Application of amphotericin B induced a significant higher daily urinary enzyme activity of the renal tubular enzymes N-acetyl-beta-glucosaminidase (NAG), beta-glucuronidase (GRS), alanine-aminopeptidase (AAP) and gamma-glutamyltransferase (GGT) in comparison with controls and sodium deoxycholate treated animals as well. A significant increase in the renal excretion of NAG, GRS, AAP and GGT occurred after the first day of amphotericin B treatment and continued until the fourth day. Following treatment for 4 days with amphotericin B urine AAP activity amounted to 69 +/- 19 U g-1 creatinine, control: 39 +/- 7 U g-1 creatinine (P < 0.05). After 4 days GGT excretion increased to 803 +/- 238 U g-1 creatinine, control: 445 +/- 106 U g-1 creatinine (P < 0.05). At the fourth day NAG excretion was 80 +/- 39 U g-1 creatinine, control: 23 +/- 5 U g-1 creatinine (P < 0.05) and GRS 724 +/- 604 U g-1 creatinine (amphotericin B), control: 276 +/- 158 U g-1 creatinine (P < 0.05). Treatment with amphotericin B decreased the creatinine clearance significantly: 0.94 +/- 0.16 ml-1 min-1 vs. control 1.35 +/- 0.29 ml-1min-1 (P < 0.05). Fractional sodium and potassium excretion was not influenced by amphotericin B. The application of sodium deoxycholate had no influence on urinary renal tubular enzyme activity. The results show that amphotericin B application induces early enzymuria of renal tubule enzymes suggesting damage of proximal renal tubules.

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“…The endosteal osteoblast population and the vasculature near the periosteal surface in flight rats showed more pronounced changes in cortical bone but no difference in the cell membrane-associated ALP activity than in metaphyseal bone compared with ground controls. Ansermet et al (1980) measured the urinary excretion of enzyme ALP Tsompos et al in unanesthetized adult male Wistar rats within 48 h after either a single injection of mercuric chloride (HgCl 2 ), or of gentamicin, or of tobramoycin, or after 30 min of clamping of both renal arteries. The excretion of ALP was increased significantly above control levels after renal ischemia or the nephrotoxic agents; the increase was dose-related after HgCl 2 .…”

Section: Discussionmentioning

confidence: 99%

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

TSOMPOS¹,

PANOULİS²,

TOUTOUZAS³

et al. 2014

jecm

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Article HistoryReceived 16 / 03 / 2014 Accepted 10 / 05 / 2014The aim of this experimental study was to examine the effect of the antioxidant drug "U-74389G", on rat model and particularly in a liver ischemia reperfusion (IR) protocol. The beneficial effect or non-effectiveness of that molecule were studied biochemically using mean blood alkaline phosphatase levels (ALP). Forty rats of mean weight 231.875 gr were used in the study. ALP levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with administration of the drug U-74389G in groups C and D. U-74389G administration non-significantly increased the ALP levels by 19.76%±15.06% (p=0.1784), but by 31.91%±7.69% (p=0.0001) for predicted values. Reperfusion time non-significantly decreased the ALP levels by 25.21%±14.83% (p=0.0675), also by 6.38%±9.30% (p=0.3650) for predicted values. However, U-74389G administration and reperfusion time together non-significantly increased the ALP levels by 8.08%±9.19% (p=0.3696), but also by 17.75%±4.79% (p=0.0005) for predicted values. This study indicates that U-74389G administration and its interaction with reperfusion time non-significantly increased short-term effects on ALP levels. However, the predicted ALP values adjusted for rats weight, kept significantly increased ones, which stand for the restorating effects of U-74389G, that are slower than it seems.

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Urinary Enzyme Excretion and Changes in Renal Functions Induced by Toxic Substances or by Renal Ischemia in Rats

Cited by 3 publications

References 14 publications

The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

Enzymuria following amphotericin B application in the rat

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

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