Urinary compounds in autism

Alcorn, Alyssa M.; Berney, T. P.; Bretherton, Karen; Mills, Malcolm J.; Savery, Dawn; Shattock, Paul

doi:10.1111/j.1365-2788.2003.00554.x

Cited by 12 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Abnormal levels of IAcrGly have been reported in a number of conditions including the photodermatitic condition, polymorphous light eruption (Marklova et al, 1975) and Hartnup disease (Jepson, 1965). Based on differing symptom phenotypes, various levels of IAcrGly have been detected in the urine of people with pervasive developmental disorder (PDD) (Shattock and Whiteley, 2002;Alcorn et al, 2004), although the precise relationship to the syndrome is unknown. PDD includes a number of heterogeneous conditions including autism (Kanner, 1943) and Asperger syndrome (Asperger, 1944).…”

Section: Introductionmentioning

confidence: 99%

Development and reproducibility of a novel high‐performance liquid‐chromatography monolithic column method for the detection and quantification of trans‐indolyl‐3‐acryloylglycine in human urine

Carr

Whiteley

Shattock

2009

Biomedical Chromatography

View full text Add to dashboard Cite

Elevated levels of trans-indolyl-3-acryloylglycine (IAcrGly) have been reported in the urine of people with various conditions including pervasive developmental disorders (PDDs) such as autism and Asperger syndrome. Reversed-phase high-performance liquid chromatography with ultra-violet detection using traditional particle silica-based columns subsequent to solid-phase extraction (SPE) has been the preferred assay method; requiring long analytical run times, high flow rates and high solvent usage. Recent developments in monolithic HPLC column technology facilitated the development of a novel analytical method, for the detection and quantification of urinary IAcrGly. The revised method eliminates the requirement for SPE pre-treatment, reduces sample run-time and decreases solvent volumes. Five urine samples from people diagnosed with PDD were run in quadruplicate to test the intra- and inter-day reliability of the new method based on retention time, peak area and peak height for IAcrGly. Detection was by UV with IAcrGly confirmation by MS/MS-MS. Relative standard deviations showed significant improvement with the new method for all parameters. The new method represents a major advancement in the detection and quantification of IAcrGly by reducing time and cost of analysis whilst improving detection limits and reproducibility.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and reproducibility of a novel high‐performance liquid‐chromatography monolithic column method for the detection and quantification of trans‐indolyl‐3‐acryloylglycine in human urine

Carr

Whiteley

Shattock

2009

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Soluble CD26 in the plasma was found to be only insignificantly different in one small study (Hunter, O'Hare, Herron, Fisher, & Jones, 2003), indicating that this deficiency may be primarily localised in the intestine. There is mounting evidence of the presence of caseomorphin and gluteomorphin, casein and gluten metabolites and other opioid peptides in the urine, (Alcorn et al, 2004;Cade, 2000;Reichelt, 1990) which together with the response of some children to GFCF diets, (Knivsberg, Reichelt, Høien, & Nødland, 2002) as is the case with Child A, further supports this possible connection.…”

Section: Discussionmentioning

confidence: 97%

PANDAS (Pediatric Autoimmune Neuropsychiatric Disease Associated with Streptococcus) in Autism?: A Case History

Cosford¹

2009

E-JAP

View full text Add to dashboard Cite

Pediatric Autoimmune Neuropsychiatric Disease Associated with Streptococcus is a recently described neuropsychiatric disorder in children, characterised by the onset of psychiatric symptoms accompanied by abnormal streptococcal serology in prepubertal children. A case of a young boy diagnosed with autism, with symptoms and serology previously described as consistent with this disorder but no apparent history of streptococcal illness, is discussed. In this case, colonic dysbiosis with overgrowth of streptococcal and enterococcal species is identified and the child is treated with probiotics and supplemental nutrients with good clinical response.

show abstract

“…Therefore, our journey delving into the etiology of ASD initially involved an exploration into the idea of increased intestinal permeability [19], also referred to as the "leaky gut syndrome." This syndrome describes the passage of potentially bioactive peptides, created from the incomplete breakdown of the proteins through the intestinal membrane, into the blood stream and ultimately into the brain through the blood-brain barrier (BBB) [20][21][22][23]. The combined work of Panksepp [15], Shattock [17,18,24,25], Waring [26], Gardner [27], and Reichelt [28] caused us to pursue this area of research.…”

Section: The Opioid Excess Theory Of Autismmentioning

confidence: 99%

Stercobilin: A Putative Link between Autism and Gastrointestinal Distress?

Wood¹,

Charlebois²,

Sekera³

et al. 2019

Autism Spectrum Disorders - Advances at the End of the Second Decade of the 21st Century

View full text Add to dashboard Cite

Despite the increasing prevalence for its diagnosis in children, there are no clinical biomarkers of autism spectrum disorders (ASD). Herein a research journey is described that began by seeking evidence for the opioid excess theory of autism using mass spectrometry methods to screen human urine specimens and has evolved into the discovery of promising murine fecal biomarkers for ASD. Our results are consistent with an emerging body of evidence that shows that intestinal microflora from ASD subjects can be distinguished from controls, suggesting that metabolite differences due to the action of intestinal microbes may provide a means to identify ASD biomarkers.

show abstract

Urinary compounds in autism

Abstract: The results would support the idea of a biological marker in prepubertal children and that it may be absent in, or obscured by the presence of severe LD.

Cited by 12 publications

References 8 publications

Development and reproducibility of a novel high‐performance liquid‐chromatography monolithic column method for the detection and quantification of trans‐indolyl‐3‐acryloylglycine in human urine

Development and reproducibility of a novel high‐performance liquid‐chromatography monolithic column method for the detection and quantification of trans‐indolyl‐3‐acryloylglycine in human urine

PANDAS (Pediatric Autoimmune Neuropsychiatric Disease Associated with Streptococcus) in Autism?: A Case History

Stercobilin: A Putative Link between Autism and Gastrointestinal Distress?

Contact Info

Product

Resources

About