Urinary chitinase 3-like 1 and intestinal fatty-acid binding proteins are not elevated in children with inflammatory bowel disease

Abstract

consent and assent were obtained from parents and children, respectively. Participants were categorized into 5 groups: active IBD, inactive IBD, non-IBD, healthy sibling (HS), and celiac disease (CeD). Patients with IBD were diagnosed based on the revised Porto criteria. 1 The active IBD group included those with newly diagnosed untreated IBD or those with relapsed disease requiring treatment to reinduce remission. The inactive IBD group included participants with known IBD who were in clinical remission based on clinical indexes (Pediatric Crohn' s Disease Activity Index [PCDAI] < 10 for CD or Pediatric Ulcerative Colitis Activity Index [PUCAI] < 10 for UC) and had no alteration to their IBD medications 3 months prior to enrolment. Children who underwent investigations for gastrointestinal symptoms and in whom IBD was excluded were categorized as the non-IBD group. Siblings of children with known IBD who were reported to be healthy were enrolled into the HS group. Children with newly diagnosed untreated CeD (based on any positive celiac serologies and histological modified Marsh-Oberhuber classification ≥ 2) were included as a disease control group.Baseline demographics including clinical (PCDAI and PU-CAI) and endoscopic disease activity (Simple Endoscopic Score for CD [SES-CD] and Mayo endoscopic score) were recorded. All disease groups provided baseline blood and urine samples, whilst HS group provided a urine sample only. Urine samples were collected and stored at 4°C within 24 hours of participant' s clinic appointment. Received samples were immediately aliquoted and stored at -80°C. Samples for analysis were