Urinary aquaporin-2 in children with acute pyelonephritis

Rodionova, Elena A.; Aa, Kuznetsova; Ei, Shakhmatova; Np, Prutskova; Nielsen, Søren; Holtbäck, Ulla; IuV, Natochin; Zelenina, Marina

doi:10.1007/s00467-005-2101-8

Cited by 16 publications

(19 citation statements)

References 25 publications

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“…To date, no data have been published on renal excretion of aquaporin 2 in the urine, or the contribution of this protein to the regulation of water balance in neonatal farm animals. The only information on this subject comes from the studies on human and rat neonates (Tsukahara et al 1998a, b;Valenti et al 2000;Bonilla-Felix 2004;Radionova et al 2006;Iacobelli et al 2010;Li et al 2011). In this work, we report that expression of AQP2 in the urine of calves significantly increased in the first month of life after the administration of additional portion of lactose with milk replacer.…”

Section: Discussionmentioning

confidence: 48%

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Aquaporin 2: Identification and analysis of expression in calves’ urine during their first month of life

et al. 2014

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Michałek, K., Dratwa-Chałupnik, A., Ciechanowicz, A. K. and Malinowski, E. 2014. Aquaporin 2: Identification and analysis of expression in calves’ urine during their first month of life. Can. J. Anim. Sci. 94: 653–659. Aquaporin 2 (AQP2) is a vasopressin-regulated water channel of the renal collecting duct and is excreted into urine. Renal excretion of AQP2 has been studied in humans, dogs, rats and mice. The aim of this study was to identify and analyze expression of AQP2 in calves’ urine. Short and controlled diarrhea was induced in the calves in order to determine any change in renal excretion of AQP2 and to evaluate a possible role of this channel in water balance during the neonatal period. For this purpose, an additional portion of lactose was administered to the calves with a milk replacer. The present work shows that after transient diarrhea associated with excessive supply of lactose in the diet, a significant increase in renal AQP2 excretion was reported in the first 3 wk of the calves’ lives. Renal excretion of this protein in the urine increased significantly despite the absence of changes in the concentration of vasopressin or the osmotic pressure of the blood plasma. The increase observed in the expression of the protein might be associated with the reduction of the total Ca2+ concentration in the blood plasma of calves. The results clearly indicate a different mechanism regulating urinary excretion of AQP2 in newborn calves, hitherto unknown. Although elevated renal excretion of AQP2 was observed, there was no increase in the osmolality of the urine in the calves tested.

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Section: Discussionmentioning

confidence: 48%

“…In a study conducted in newborn rats it was shown that the reduced ability to retain water in the neonate kidney also results from low expression of AQP2 in the collecting ducts. The renal tubule expression of AQP2 and AQP2 excretion in neonates is about 50% lower than in adults (Yasui et al 1996;Tsukuhara et al 1998b;Radionova et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Aquaporin 2: Identification and analysis of expression in calves’ urine during their first month of life

et al. 2014

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“…AVP also stimulates the reabsorption of NaCl mediated by the epithelial sodium channel (ENaC) and activates the cAMP-sensitive cystic fibrosis transmembrane conductance regulator (CFTR) Cl − conductance in cultured renal collecting duct cells ( 11 – 13 ). Children with pyelonephritis exhibit increased levels of circulating AVP and develop polyuria with urinary concentrating defect, probably related to acute renal interstitial inflammation ( 14 – 16 ). However, this does not exclude that vasopressin may produce unexpected biological effects on renal cells independently of its antidiuretic action.…”

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confidence: 99%

Hormonal control of the renal immune response and antibacterial host defense by arginine vasopressin

Chassin

Hornef

Bens

et al. 2007

The Journal of Experimental Medicine

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Ascending urinary tract infection (UTI) and pyelonephritis caused by uropathogenic Escherichia coli (UPEC) are very common infections that can cause severe kidney damage. Collecting duct cells, the site of hormonally regulated ion transport and water absorption controlled by vasopressin, are the preferential intrarenal site of bacterial adhesion and initiation of inflammatory response. We investigated the effect of the potent V2 receptor (V2R) agonist deamino-8-D-arginine vasopressin (dDAVP) on the activation of the innate immune response using established and primary cultured collecting duct cells and an experimental model of ascending UTI. dDAVP inhibited Toll-like receptor 4–mediated nuclear factor κB activation and chemokine secretion in a V2R-specific manner. The dDAVP-mediated suppression involved activation of protein phosphatase 2A and required an intact cystic fibrosis transmembrane conductance regulator Cl− channel. In vivo infusion of dDAVP induced a marked fall in proinflammatory mediators and neutrophil recruitment, and a dramatic rise in the renal bacterial burden in mice inoculated with UPECs. Conversely, administration of the V2R antagonist SR121463B to UPEC-infected mice stimulated both the local innate response and the antibacterial host defense. These findings evidenced a novel hormonal regulation of innate immune cellular activation and demonstrate that dDAVP is a potent modulator of microbial-induced inflammation in the kidney.

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“…Relative urinary AQP2 level in the samples was measured by dot immunoblotting as described previously [13]. Urine was applied on a nitrocellulose membrane using Bio-Dot microfiltration apparatus (Bio-Rad Laboratories, Sundbyberg, Sweden), and AQP2 was detected using rabbit polyclonal antibodies LL358 and ECL Plus Western blotting analysis system.…”

Section: Methodsmentioning

confidence: 99%

Urinary aquaporin-2 excretion during early human development

Zelenina

Glorieux³

et al. 2006

Pediatr Nephrol

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This study was undertaken to assess one of the determinants of kidney concentrating capacity, aquaporin-2 (AQP2), in order to understand the physiopathology of water balance in newborn babies. Urinary AQP2 excretion has been shown to be proportional to AQP2 level in the apical plasma membrane of the kidney collecting ducts and has been suggested as a marker of vasopressin (AVP) action. Urinary AQP2 excretion in the early postnatal period and at 3 weeks of age was measured in 123 neonates admitted during a 6-month period to the neonatal intensive care unit of the Children's Hospital of Toulouse, France. Clinical and biochemical data were collected for each child. During the first days after birth, higher urinary AQP2 was observed in boys than in girls (P=0.01) and positively correlated with urinary sodium/potassium (Na/K) ratio (r=0.33, P=0.01). When the babies had reached 3 weeks of age, urinary AQP2 was proportional to the gestational age at birth (r=0.33, P=0.0068) and daily weight gain (r=0.36, P=0.003). It did not correlate with urinary osmolality, which was overall very low in all babies. Urinary AQP2 was decreased in conditions of impaired renal function (r=-0.42, P=0.0005) and acidosis (P=0.03). Prenatal corticosteroid treatment had no significant impact on urinary AQP2 level. Our data show that urinary AQP2 correlates with the overall maturity of tubular function in human neonates. In babies at this early age, urinary AQP2 cannot serve as a direct marker of the renal action of AVP but reflects AQP2 expression level associated with different physiopathological conditions.

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Urinary aquaporin-2 in children with acute pyelonephritis

Cited by 16 publications

References 25 publications

Aquaporin 2: Identification and analysis of expression in calves’ urine during their first month of life

Aquaporin 2: Identification and analysis of expression in calves’ urine during their first month of life

Hormonal control of the renal immune response and antibacterial host defense by arginine vasopressin

Urinary aquaporin-2 excretion during early human development

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