Urinary 8-oxo-7,8-dihydroguanosine as a Potential Biomarker of Aging

Gan, Wei; Liu, Xinle; Yu, Ting; Zou, Yuangao; Li, Tingting; Wang, Shuang; Deng, Jin; Wang, Lanlan; Cai, Jian‐Ping

doi:10.3389/fnagi.2018.00034

Cited by 45 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, a new biomarker, urinary 8-oxo-7, 8-dihydroguanosine (8-oxoGsn) has emerged as a promising biomarker to assess the patient physiologic age as it increases progressively with age [42]. If validated, 8-oxoGsn could be employed to assess older cancer patients functional status to determine whether systemic therapy could be integrated with radiotherapy for patients with locally advanced or systemic disease.…”

Section: Discussionmentioning

confidence: 99%

Challenges Facing Radiation Oncologists in The Management of Older Cancer Patients: Consensus of The International Geriatric Radiotherapy Group

Popescu

Karlsson²,

Vinh-Hung

et al. 2019

Cancers

View full text Add to dashboard Cite

The management of older cancer patients remains difficult because of data paucity. Radiation oncologists need to identify potential issues which could affect treatment of those patients. A workshop was organized in Barcelona among international radiation oncologists with special interest in the management of older cancer patients on April 22, 2018. The following consensus was reached: 1. Older cancer patients often faced unconscious discriminating bias from cancer specialists and institutions because of their chronological age. 2. Advances in radiotherapy techniques have allowed patients with multiple co-morbidities precluding surgery or systemic therapy to achieve potential cure in early disease stages. 3. The lack of biomarkers for frailty remains an impediment to future research. 4. Access to healthcare insurance and daily transportation remains an issue in many countries; 5. Hypofractionation, brachytherapy, or stereotactic techniques may be ideally suited for older cancer patients to minimize transportation issues and to improve tolerance to radiotherapy. 6. Patients with locally advanced disease who are mentally and physically fit should receive combined therapy for potential cure. 7. The role of systemic therapy alone or combined with radiotherapy for frail patients needs to be defined in future clinical trials because of targeted agents or immunotherapy may be less toxic compared to conventional chemotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Challenges Facing Radiation Oncologists in The Management of Older Cancer Patients: Consensus of The International Geriatric Radiotherapy Group

Popescu

Karlsson²,

Vinh-Hung

et al. 2019

Cancers

View full text Add to dashboard Cite

show abstract

“…cross-sectional and mixed-longitudinal studies [69,70,86,[98][99][100][101][102], but not always [23,55,[103][104][105][106]. For example, urinary 8-OHdG increased with age in all patients with muscular dystrophy [86] and between certain age categories in a large cross-sectional sample of men and women [100], but did not increase with age in pollution-exposed silica miners [106]. https://doi.org/10.1371/journal.pone.0238066.g005 Table 4.…”

Section: Plos Onementioning

confidence: 99%

Urinary markers of oxidative stress respond to infection and late-life in wild chimpanzees

et al. 2020

View full text Add to dashboard Cite

Oxidative stress (OS) plays a marked role in aging and results from a variety of stressors, making it a powerful measure of health and a way to examine costs associated with life history investments within and across species. However, few urinary OS markers have been examined under field conditions, particularly in primates, and their utility to non-invasively monitor the costs of acute stressors versus the long-term damage associated with aging is poorly understood. In this study, we examined variation in 5 urinary markers of oxidative damage and protection under 5 validation paradigms for 37 wild, chimpanzees living in the Kibale National Park, Uganda. We used 924 urine samples to examine responses to acute immune challenge (respiratory illness or severe wounding), as well as mixed-longitudinal and intra-individual variation with age. DNA damage (8-OHdG) correlated positively with all other markers of damage (F-isoprostanes, MDA-TBARS, and neopterin) but did not correlate with protection (total antioxidant capacity). Within individuals, all markers of damage responded to at least one if not both types of acute infection. While OS is expected to increase with age, this was not generally true in chimpanzees. However, significant changes in oxidative damage were detected within past-prime individuals and those close to death. Our results indicate that OS can be measured using field-collected urine and integrates short-and long-term aspects of health. They further suggest that more data are needed from long-lived, wild animals to illuminate if common age-related increases in inflammation and OS damage are typical or recently aberrant in humans.

show abstract

“…More and more evidence supports that RNA oxidation plays a substantial role in the progress of multiple diseases, such as Alzheimer's disease 1 , diabetes 2 and aging 3 , and plenty of studies have reported microbial pathogens can induce the increase of oxidative stress and inflammation [4][5][6][7] . However, only a few studies have reported RNA oxidation caused by microbial pathogens 8 .…”

Section: Backgoundmentioning

confidence: 99%

Systemic RNA oxidation can be used as an index of clinical infection and prognosis in a Vibrio parahaemolyticus Challenge Rat Model

Pian

Nie

Wang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: More and more evidence supports the concept that RNA oxidation plays a substantial role in the progress of multiple diseases; however, only a few studies have reported RNA oxidation caused by microbial pathogens. Urinary 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn), which are broadly used as indicators of oxidative damage of RNA and DNA, were analyzed in this study to determine which can be used as an index of clinical infection and prognosis in a Vibrio parahaemolyticus challenge rat model. In this study, twenty-four speciﬁc-pathogen-free (SPF) male SD rats were randomly divided into two groups: an infection group and a phosphate-buffered saline (PBS) control group. An LC-MS/MS-based system was established to determine the 8-oxo-dGsn and 8-oxo-Gsn contents of urine samples. The immunohistochemistry (IHC) was used to determine the 8-oxoguanine in nuclear DNA and in cellular RNA of different tissues of rats. Hematoxylin-eosin (H&E) stain was used to analyze intestinal inflammation. Sysmex XS-1000i hematology was used to analyze WBCs in blood. Luminex and ELISA were used to detect the level of inflammatory factors in serum. In addition, body temperatures, body weights, bacterial burden of Vibrio parahaemolyticus were also detected. Results: The level of urinary and tissular 8-oxo-Gsn rather than 8-oxo-dGsn was significantly increased after infection with V. parahaemolyticus compared with PBS control. Stimultaneously, intestinal inflammation, the numbers of white blood cells (WBCs) and inflammatory factors (including CRP, IL-6, IL-1β, TNF-α, IL-10 and IL-17A) were increased sharply. What is clinical significance is that the trend of urinary 8-oxo-Gsn was consistent with WBCs or inflammation. It is more important that the concentration of urinary 8-oxo-Gsn in the infection group was positively correlated with WBCs and inflammatory cytokines. Conclusion: Our results demonstrated that 8-oxo-Gsn can be used as a more effective biomarker of clinical infection and prognosis compared with classic clinical indicators such as IL-6 and TNF-α.

show abstract

Urinary 8-oxo-7,8-dihydroguanosine as a Potential Biomarker of Aging

Cited by 45 publications

References 27 publications

Challenges Facing Radiation Oncologists in The Management of Older Cancer Patients: Consensus of The International Geriatric Radiotherapy Group

Challenges Facing Radiation Oncologists in The Management of Older Cancer Patients: Consensus of The International Geriatric Radiotherapy Group

Urinary markers of oxidative stress respond to infection and late-life in wild chimpanzees

Systemic RNA oxidation can be used as an index of clinical infection and prognosis in a Vibrio parahaemolyticus Challenge Rat Model

Contact Info

Product

Resources

About