Urinary 8-hydroxy-2′-deoxyguanosine as a novel biomarker of inflammatory activity in patients with cardiac sarcoidosis

Kobayashi, Shigeki; Myoren, Takeki; Oda, Shinobu; Inari, Makana; Ishiguchi, Hironori; Murakami, Wakako; Fukuda, Masakazu; Tanaka, Takeo; Okuda, Shinichi; Nao, Tomoko; Doi, Masahiro; Yamada, Jiro; Okamura, Takayuki; Hoshii, Yoshinobu; Suga, Kazuyoshi; Matsuzaki, Masunori; Yano, Masafumi

doi:10.1016/j.ijcard.2015.04.144

Cited by 21 publications

(23 citation statements)

References 29 publications

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“…Body weight (g) Testis weight (g) Epididymis weight (g) Organs coefficient diac sarcoidosis [19]. Animal and human studies revealed that the prevalence of 8-OHdG-immunostained germ cells was positively correlated with clinical grades of varicocele [20,21].…”

Section: Groupsmentioning

confidence: 99%

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

Feng

Huang

Yang

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Groupsmentioning

confidence: 99%

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

Feng

Huang

Yang

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…A number of analytical techniques for measurement of urinary 8-OHdG have been developed, including enzyme-linked immunosorbent assay (ELISA)333435, capillary electrophoresis with various detectors (e.g., electrochemical or ultraviolet detection, CE-ECD or CE-UV)363738, high-performance liquid chromatography with electrochemical detection (HPLC-ECD)3940, gas chromatography-mass spectrometry (GC-MS)414243, and liquid chromatography-tandem mass spectrometry (LC-MS/MS)33344445464748495051. ELISA is the most convenient method and has received widespread use, but lack of specificity seems to be the main problem and several reports have demonstrated that ELISA frequently overestimates urinary 8-OHdG levels compared with the concentrations measured by chromatography-based techniques.…”

mentioning

confidence: 99%

Association between Oxidative DNA Damage and Risk of Colorectal Cancer: Sensitive Determination of Urinary 8-Hydroxy-2′-deoxyguanosine by UPLC-MS/MS Analysis

Guo

Wang

et al. 2016

Sci Rep

120

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Oxidative DNA damage plays crucial roles in the pathogenesis of numerous diseases including cancer. 8-hydroxy-2′-deoxyguanosine (8-OHdG) is the most representative product of oxidative modifications of DNA, and urinary 8-OHdG is potentially the best non-invasive biomarker of oxidative damage to DNA. Herein, we developed a sensitive, specific and accurate method for quantification of 8-OHdG in human urine. The urine samples were pretreated using off-line solid-phase extraction (SPE), followed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. By the use of acetic acid as an additive to the mobile phase, we improved the UPLC-MS/MS detection of 8-OHdG by 2.7−5.3 times. Using the developed strategy, we measured the contents of 8-OHdG in urine samples from 142 healthy volunteers and 84 patients with colorectal cancer (CRC). We observed increased levels of urinary 8-OHdG in patients with CRC and patients with tumor metastasis, compared to healthy controls and patients without tumor metastasis, respectively. Additionally, logistic regression analysis and receiver operator characteristic (ROC) curve analysis were performed. Our findings implicate that oxidative stress plays important roles in the development of CRC and the marked increase of urinary 8-OHdG may serve as a potential liquid biomarker for the risk estimation, early warning and detection of CRC.

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“…[19][20][21] The quantitative analysis of 18 F-FDG uptake in the lesion was based on the maximum standardized uptake value (SUVmax) per focus, as described previously. [19][20][21] The intensity of myocardial FDG uptake was quantified by measuring the SUV in all 17 segments, in accordance with the Scientific Statement from American Heart Association. Analysis of myocardial FDG uptake was performed by recognizing the endomyocardial and epicardial borders and by subdividing the LV in each segment.…”

Section: Evaluation Of Va and LV Wall Thinning In Patients With Activmentioning

confidence: 99%

“…Other Neurohumoral/Inflammatory Factors U-8-OHdG concentrations, a marker of oxidative stress, were measured using an enzyme-linked immunosorbent assay kit (Japan Institute for the Control of Aging, Fukuroi, Japan) with anti-8-OHdG antibody (N45.1), as described previously. [19][20][21][22] Other neurohumoral and inflammatory factors were measured as described previously. [19][20][21][22] Steroid Therapy for Active CS Corticosteroid treatment (prednisolone) was initiated according to existing Japanese protocols as follows: 30 mg/day for 4 weeks with gradual tapering of the dose to 5-10 mg every other day over 6 months to establish the minimal heart, which comprised viable myocardium as evaluated by other modalities.…”

Section: Measurement Of Urinary 8-hydroxy-2'-deoxyguanosine (U-8-ohdgmentioning

confidence: 99%

Detection of Active Inflammation Status Around Ventricular Aneurysms in Patients With Cardiac Sarcoidosis

Nanno

Kobayashi

Yoshitomi

et al. 2019

Circ J

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Background: Little is known about the pattern of isotope accumulation in the heart on 18 F-fluorodeoxyglucose (18 F-FDG) positron emission tomography in patients with cardiac sarcoidosis (CS) complicated by ventricular aneurysm (VA). Methods and Results: We prospectively enrolled 82 consecutive patients with CS; 54 patients with active CS (presence of abnormal 18 F-FDG accumulation in the heart) were subdivided into VA (n=17) and non-VA groups (n=37). Strong 18 F-FDG accumulation surrounding the VA and its disappearance in the VA center was observed in all patients with VA, probably because of scar formation at the VA. Peak standardized uptake value was higher around the VA than in the VA center (5.1±2.1 vs. 2.2±0.6, P=0.0003) and the VA center had no 18 F-FDG accumulation (VA center: 2.2±0.6 vs. control area: 2.1±0.6, P=0.37). On the other hand, in non-VA patients with LV wall thinning (n=28), 18 F-FDG accumulation was significantly high, even in the area of LV wall thinning (LV wall thinning area: 3.1±0.8 vs. control area: 2.0±0.6, P=0.00002). Conclusions: A pattern of strong 18 F-FDG accumulation surrounding the VA and its disappearance in the VA center might be characteristic in patients with CS complicated by VA. Careful attention to FDG uptake would further elucidate CS pathophysiology and aid in the early treatment of VA.

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Urinary 8-hydroxy-2′-deoxyguanosine as a novel biomarker of inflammatory activity in patients with cardiac sarcoidosis

Cited by 21 publications

References 29 publications

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

Association between Oxidative DNA Damage and Risk of Colorectal Cancer: Sensitive Determination of Urinary 8-Hydroxy-2′-deoxyguanosine by UPLC-MS/MS Analysis

Detection of Active Inflammation Status Around Ventricular Aneurysms in Patients With Cardiac Sarcoidosis

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