Urinary 8‐hydroxy‐2‘‐deoxyguanosine (8‐OHdG) Levels in Women with or without Gynecologic Cancer*

Yamamoto, Takara; Hosokawa, Kiyohito; Tamura, Takeyuki; Kanno, Hiroshi; Urabe, Mamoru; Honjo, Hideo

doi:10.1111/j.1447-0756.1996.tb00989.x

Cited by 48 publications

(35 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In an epidemiological study, Kasai et al reported that when subjects were divided into three age groups, the urinary 8-OHdG of the younger group was higher than that of the older group using multiple regression analysis 3) . Yamamoto et al reported that the urinary 8-OHdG of subjects tended to decrease as their age increased 19) , but other studies have reported no significant results about age 2,7,20,21) . In the present study, there were no significant correlations between age and the differences in 8-OHdG at the three different storage temperatures.…”

Section: Discussionmentioning

confidence: 96%

The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature

Matsumoto

Ogawa

Yoshida

et al. 2008

Journal of Occupational Health

View full text Add to dashboard Cite

The Stability of the Oxidative Stress Marker, Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), when Stored at Room Temperature: Yuki MATSUMOTO, et al. National Institute of Occupational Safety and Health-We examined the stabilities of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) stored at room temperature (25°C) for 24 h or at -80°C for 800 days. Subjects were 19 males and 17 females aged 23-58 yr for the 24-h study, and 9 males and 4 females aged 24-54 yr for the 800-day study. We obtained information on the subjects by questionnaires and interviews. The level of urinary 8-OHdG was measured by HPLC using two-step separations. There were no significant changes of amount of urinary 8-OHdG under either storage conditions. We conclude that urine samples can be stored at 25°C and below for 24 h, when the research purpose includes the determination of urinary 8-OHdG. Urinary 8-OHdG was also stable for over two years when stored at -80°C. (J Occup Health 2008; 50: 366-372)

show abstract

Section: Discussionmentioning

confidence: 96%

The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature

Matsumoto

Ogawa

Yoshida

et al. 2008

Journal of Occupational Health

View full text Add to dashboard Cite

show abstract

“…This indicates a 50% increased rate of oxidative DNA damage from smoking, in addition to the other wellknown health hazards of smoking [11]. The urinary 8OHdG levels are also significantly higher in patients with gynecologic cancer, lung cancer and colorectal cancer, brain tumor than that in control subjects [12,13].…”

Section: Introductionmentioning

confidence: 93%

Capillary electrophoresis with end‐column amperometric detection of urinary 8‐hydroxy‐2'‐deoxyguanosine

et al. 2003

View full text Add to dashboard Cite

Urinary 8-hydroxy-2'-deoxyguanosine (8OHdG) is an excellent marker of oxidative DNA damage. Until now, urinary 8OHdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 8OHdG by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. A single-step solid-phase extraction procedure was optimized and used for extracting 8OHdG from human urine. To improve the sensitivity of this method, a new focusing technique based on a dynamic pH junction was used. The limit of detection was 20 nM (signal-to-noise ratio S/N = 3), the linear range was 50 nM-10 microM, and the correlation coefficient was better than 0.999. The relative standard deviation (RSD) was found to be 0.57% for migration time, and 4.79% for peak current. To show the usefulness of the method, the urinary concentration of 8OHdG in nine healthy persons and ten cancer patients was determined. The urinary concentration of 8OHdG in cancer patients was significantly higher than that in healthy persons.

show abstract

“…Toyokuni et al reported that human carcinoma cells (breast, lung, liver, kidney, brain, stomach, ovary) have a higher content of 8-OHdG than adjacent nontumorous tissues (11). Moreover, investigators have reported a high concentration of 8-OHdG in urine samples from patients with carcinoma of female genitalia (12), malignant breast tissues with invasive ductal carcinoma (10), colorectal tumor tissues (13), gastric cancer tissues (14), and lung cancer tissues (15). They hypothesized that the tumor cells themselves produce reactive oxygen species spontaneously resulting in an increase of 8-OHdG in DNA.…”

Section: Introductionmentioning

confidence: 99%

Effect of a 4-Month Tea Intervention on Oxidative DNA Damage among Heavy Smokers

Hakim

Harris

Chow

et al. 2004

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

Glutathione S-transferase (GST), a member of the phase II group of xenobiotic metabolizing enzymes, has been intensively studied at the levels of phenotype and genotype. The GST 1 (GSTM1) and GST 1 (GSTT1) genes have a null-allele variant in which the entire gene is absent. The null genotype for both enzymes has been associated with many different types of tumors. The aim of this study was to determine the possible differences in increased oxidative stress susceptibility to smoking within the GSTM1 and GSTT1 genotypes and the impact of high tea drinking on this. We designed a Phase II randomized, controlled, three-arm tea intervention trial to study the effect of high consumption (4 cups/day) of decaffeinated green or black tea, or water on oxidative DNA damage, as measured by urinary 8-hydroxydeoxyguanosine (8-OHdG), among heavy smokers over a 4-month period and to evaluate the roles of GSTM1 and GSTT1 genotypes as effect modifiers. A total of 133 heavy smokers (100 females and 33 males) completed the intervention. GSTM1 and GSTT1 genotype statuses were determined with a PCR-based approach. Multiple linear regression models were used to estimate the main effects and interaction effect of green and black tea consumption on creatinine-adjusted urinary 8-OHdG, with or without adjustment for potential confounders. Finally, we studied whether the effect of treatment varied by GSTM1 and GSTT1 status of the individual. Although there were no differences in urinary 8-OHdG between the groups at baseline, the between-group 8-OHdG levels at month 4 were statistically significant for GSTM1-positive smokers (P ‫؍‬ 0.05) and GSTT1-positive smokers (P ‫؍‬ 0.02). GSTM1-positive and GSTT1-positive smokers consuming green tea showed a decrease in urinary 8-OHdG levels after 4 months. Assessment of urinary 8-OHdG after adjustment for baseline measurements and other potential confounders revealed significant effect for green tea consumption (P ‫؍‬ 0.001). The change from baseline was significant in both GSTM1-positive (t ‫؍‬ ؊2.99; P ‫؍‬ 0.006) and GSTT1-positive (P ‫؍‬ 0.004) green tea groups, but not in the GSTM1-negative (P ‫؍‬ 0.07) or GSTT1-negative (P ‫؍‬ 0.909) green tea groups. Decaffeinated black tea consumption had no effect on urinary 8-OHdG levels among heavy smokers. Our data show that consumption of 4 cups of tea/day is a feasible and safe approach and is associated with a significant decrease in urinary 8-OHdG among green tea consumers after 4 months of consumption. This finding also suggests that green tea intervention may be effective in the subgroup of smokers who are GSTM1 and/or GSTT1 positive.

show abstract

Urinary 8‐hydroxy‐2‘‐deoxyguanosine (8‐OHdG) Levels in Women with or without Gynecologic Cancer*

Abstract: These results suggest that it might be possible to determine the spread of cancer to some extent by determining a patient's urinary 8-OHdG level.

Cited by 48 publications

References 7 publications

The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature

The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature

Capillary electrophoresis with end‐column amperometric detection of urinary 8‐hydroxy‐2'‐deoxyguanosine

Effect of a 4-Month Tea Intervention on Oxidative DNA Damage among Heavy Smokers

Contact Info

Product

Resources

About