Urinary 6-sulfatoxymelatonin and total antioxidant capacity increase after the intake of a grape juice cv. Tempranillo stabilized with HHP

Abstract

Red grapes contain elevated amounts of antioxidant compounds (polyphenols) that may potentially prevent cell aging, cardiovascular disease and oxidation-related disorders. Since functional drinks are presently one of the most dynamic sectors of the market, the present work was aimed at evaluating the possible antioxidant effect of an experimental grape juice in terms of urinary 6-sulfatoxymelatonin (aMT6-s) and total antioxidant capacity in young (20 ± 10 yr-old), middle-aged (45 ± 10 yr-old) and elderly (75 ± 10 yr-old) individuals. Grapes (Vitis vinifera cv. Tempranillo) were de-stemmed, racked and pressed. The juice was subsequently stabilized by high hydrostatic pressure (HHP). Participants consumed 200 mL of grape juice twice a day (as the lunch and dinner desserts) for 5 days. First-void morning urines were collected before treatment (basal values), the day immediately after the last ingestion of juice (assay), and one day afterwards (post-assay). aMT6-s and total antioxidant capacity were quantified using commercial ELISA and colorimetric assay kits, respectively. The intake of grape juice cv. Tempranillo induced a significant increase of urinary aMT6-s and total antioxidant capacity in the three groups of age analyzed as compared to their corresponding basal and post-assay values. These functional/nutraceutical properties may be of interest for a prospective commercialization of the grape juice. The novel technology used for juice stabilization may be suitable for introducing into the market a product with high sensory and nutritional quality, as it has been shown in this study.

Tryptophan-enriched cereal intake improves nocturnal sleep, melatonin, serotonin, and total antioxidant capacity levels and mood in elderly humans

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The role of imbalance between urinary hexanoyl-lysine and total antioxidant capacity levels and its relation to plasma superoxide dismutase levels in autism spectrum disorder

“…Urinary levels of TAC may be affected by dietary food intake may [26,27]. Berries, fruits, nuts, chocolate, vegetables [35], and cafe and soybean oil had the highest antioxidant values [36].…”

“…Finally, we determined the relationship between HEL and TAC in urine of individuals with ASD. Because previous clinical studies have found that urinary levels of TAC are affected by diet [26,27], we also assessed dietary nutrient intake and estimated dietary TAP.…”

Melatonin and its use in atherosclerosis and dyslipidemia

“…17 Drinking 400 mL per day of a "stabilized" grape juice also elevated the 6-sulphatoximelatonin concentration in urine. 18 Serum melatonin is rapidly hydroxylated to 6-sulphatoximelatonin in the liver, is excreted by the kidneys, and has a half-life of approximately 30 minutes.…”