Uridine phosphorylase 1 associates to biological and clinical significance in thyroid carcinoma cell lines

Abstract

Thyroid cancer incidence has been continuity increasing worldwide. Uridine phosphorylase 1 (UPP1) is a protein‐coding gene and has been detected that UPP1 was the higher expression in many solid malignancies, just as head and neck cancers, breast cancer, compared with paired normal tissue. But the act of UPP1 in thyroid cancer is not explicit. In this article, we investigate the function of UPP1 expression in thyroid cancer. The Cancer Genome Atlas (TCGA) unpaired thyroid cancer and normal RNA‐seq data were downloaded, and our paired thyroid cancer and normal samples were analysed by a polymerase chain reaction. The expression of UPP1 was regulated by transfected small interfering RNA, and the function of UPP1 was determined via migration, invasion and cell proliferation assays. Western blot assay was achieved to determine the UPP1 expression correlates with the function of 5‐FU regulate epithelial‐mesenchymal transition. The significant upregulation of UPP1 in thyroid cancer tissues compared with normal thyroid tissues was revealed by our data and TCGA data. UPP1 overexpression was significantly correlated with lymph node metastasis, tumour stage and tumour size. In the cell, experiments showed that UPP1 low expression significantly suppressed the migration, invasion and proliferation. Western blot assay proves the effect of UPP1 expression on 5‐FU regulates epithelial‐mesenchymal transition pathway. UPP1 plays a crucial oncogene in thyroid cancer. Our findings indicate that UPP1 might be a biomarker of thyroid cancer and may act by regulating epithelial‐mesenchymal transition (EMT).

Novel insight into the regulatory roles of diverse RNA modifications: Re-defining the bridge between transcription and translation

“…The expression of METTL14 is decreased in HCC, especially in metastatic HCC [119] Pancreatic cancer METTL3 N.A. METTL3 protein, m 6 A abundance and mRNA levels are much higher in tumour specimens than in para-cancerous specimens [120] YTHDF2 YAP Increased YTHDF2 promotes proliferation and suppresses migration of pancreatic cancer by destabilizing YAP mRNA [121] Lung cancer METTL3 EGFR/ TAZ METTL3 enhances the translation of EGFR and TAZ mRNA in lung cancer [ [164] mRNA modifications that can change the structure of transcripts, and the other is mRNA modifications that can regulate transcription by joining hands with a complex of enzymes, such as METTL3 or NSUN2. Considering that modifications can regulate the fate of diverse diseases, such modifications have the potential to be utilized in targeted therapy.…”

“…Finally, uridine phosphorylase 1 (UPP1) is another enzyme that can reversibly catalyse the phosphorolysis of uridine to uracil [162,163]. It has been reported that expression of UPP1 significantly depends on lymph node metastasis and tumour stage and size in patients with thyroid carcinoma [164] (Table 3, Fig. 4).…”

Identification of crucial genes of pyrimidine metabolism as biomarkers for gastric cancer prognosis

“…[ 32 ] Researches have investigated the connections between the expression of UPP1 and the prognosis of cancer patients. They demonstrated that the higher the level of UPP1 in the tumor, the worse the prognosis and the shorter the survival time of cancer patients [ 33 ]. But genes are not an isolated island, the interactions between genes form a beautiful pulsating map of life.…”

Identification of Crucial Genes of Pyrimidine Metabolism as Biomarkers for Gastric Cancer Prognosis

“…Researches have investigated the connections between the expression of UPP1 and the prognosis of cancer patients. They demonstrated that the higher the level of UPP1 in the tumor, the worse the prognosis and the shorter the survival time of cancer patients [31]. But genes are not an isolated island, the interactions between genes form a beautiful pulsating map of life.…”