Uricase Based Enzymatic Biosensor for Non‐invasive Detection of Uric Acid by Entrapment in PVA‐SbQ Polymer Matrix

RoyChoudhury, Sohini; Umasankar, Yogeswaran; Hutcheson, Joshua D.; Lev‐Tov, Hadar; Kirsner, Robert S.

doi:10.1002/elan.201800360

Cited by 29 publications

(23 citation statements)

References 67 publications

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“…[ 31,32 ] In this process, the polymer matrix can not only physically entrap the enzymes on the electrode but also improve their immobilization via electrostatic interactions between negatively charged amino acid chains of enzymes and positively charged styrylpyridinium side chains in PVA–SbQ. [ 33,34 ] Fluorescence microscopy and SEM characterizations demonstrated that the shedding of the enzyme from the electrode was remarkably reduced after rinsing (Figure 2e,f; Figures S9 and S10, Supporting Information). Based on the above advantages, the bioelectrocatalytic performance of both O 2 reduction and glucose oxidization can be effectively enhanced.…”

Section: Resultsmentioning

confidence: 99%

Designing Porous Antifouling Interfaces for High‐Power Implantable Biofuel Cell

Wang

Gao

et al. 2021

Adv Funct Materials

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Biofuel cells that can convert the chemical energy of biomass such as glucose into electricity are expected to continuously obtain energy from living organisms and solve bottlenecks of energy supply for implanted electronics. However, the use of biofuel cells is limited mainly by the sharp drop in performance after implanting in living organisms caused by biofouling and electrode surface inactivation. Herein, a simple and effective strategy to overcome these problems by designing a porous antifouling interface on biofuel cells, is demonstrated. It resists the biofouling from body fluid while sustaining reactant penetration, and also enhances immobilization of enzymes. As a result, the biofuel cell can maintain almost 100% performance after implanting in living organisms, and a maximal output power of 76.6 mW cm‐3 is achieved in vivo, which is ≈96 times of the highest performance reported to date. This strategy is universal and can be extended to the other electronic devices such as electrochemical sensors, which presents a new avenue for developing high‐performance implanted electronics.

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Section: Resultsmentioning

confidence: 99%

Designing Porous Antifouling Interfaces for High‐Power Implantable Biofuel Cell

Wang

Gao

et al. 2021

Adv Funct Materials

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“…7, represented by yellow and green arrows. There is a dynamic balance between monosodium urate and synovial fluid, and the separated monosodium urate ion immediately produces a chemical reaction when it encounters uricase [13]. Although monosodium urate is difficult to dissolve, it still has dissolution equilibrium with the aqueous solution.…”

Section: Discussionmentioning

confidence: 99%

“…According to reports, tophi are formed by the deposition of MSU. Monosodium urate is formed by replacing H + with Na + in the molecular structure, and this molecule is less soluble and thus more likely to form precipitates and deposits in human body [12,13]. There is no significant difference in molecular structure between the two compounds and the core and functional groups of the two compounds are similar.…”

Section: Introductionmentioning

confidence: 99%

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The effect of uricase on monosodium urate (MSU) crystallization, IL-1β and TNFα in monosodium urate-induced arthritis

Chen

Liang²,

Wang³

et al. 2020

Preprint

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Background : Although the symptoms of gouty arthritis could be controlled by clinical methods, it is often impossible to remove tophi from joints that cause acute gouty arthritis. Methods : In study, we injected a suspension of MSU into the knee joint of chicken, and then injected uricase solution into the joint after 4, 8 and 12 hours. Since the beginning of the experiment, we monitored the circumference of the diseased joint and local temperature change. Computer tomography(CT) examination was performed to examine the MSU in the knee joints and then measured the levels of TNFα and IL-1β in synovial fluid. Chicken knee joint fluid was collected under polarized light microscope to observe the condition of the crystals and measured the weight of MSU residued in synovial fluid. Finally, the chicken knee joint was dissected and the joint synovium was sliced. Results : The results showed that injection of MSU suspension into chicken knee joint, significantly increased IL-1β, TNFα levels (p<0.01), circumference of the joint (p<0.05) and the local skin temperature (p<0.05). Moreover, analysis of the joint fluid and synovial slice under the polarized light microscope revealed that crystals significantly reduced. Compared with groupⅡ(control group), the residual weight of MSU in the joint fluid of group Ⅲ and group Ⅳ is very small(p<0.05), but there is no significant difference between group Ⅲ and group Ⅳ(p＞0.05). Conclusions : The comprehensive analysis shows that the reaction process between uricase and MSU is similar to that uric acid. Therefore, it provides a new way for clinical treatment of acute gouty arthritis with fewer side effects and significant reduction in the frequency of attacks.

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“…In fact, there are many ways to modify enzymes on electrochemical electrodes, including physisorption, chemisorption, and entrapment [ 102 ]. RoyChoudhury et al [ 103 ] reported a flexible wearable electrochemical sensor for uric acid detection based on UOx, and the UOx was entrapped in poly(vinyl alcohol) N -methyl-4(4′-formylstyryl) pyridinium methosulfate acetal, a cationic polymer matrix. In addition, ferrocene carboxylic acid (FCA) was also introduced to assist the electron transfer between the active site of the enzyme and the surface of the sensor.…”

Section: Key Wound Marker Detectionmentioning

confidence: 99%

A Review of Recent Advances in Flexible Wearable Sensors for Wound Detection Based on Optical and Electrical Sensing

Sun

Zhang

et al. 2021

Biosensors

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Chronic wounds that are difficult to heal can cause persistent physical pain and significant medical costs for millions of patients each year. However, traditional wound care methods based on passive bandages cannot accurately assess the wound and may cause secondary damage during frequent replacement. With advances in materials science and smart sensing technology, flexible wearable sensors for wound condition assessment have been developed that can accurately detect physiological markers in wounds and provide the necessary information for treatment decisions. The sensors can implement the sensing of biochemical markers and physical parameters that can reflect the infection and healing process of the wound, as well as transmit vital physiological information to the mobile device through optical or electrical signals. Most reviews focused on the applicability of flexible composites in the wound environment or drug delivery devices. This paper summarizes typical biochemical markers and physical parameters in wounds and their physiological significance, reviews recent advances in flexible wearable sensors for wound detection based on optical and electrical sensing principles in the last 5 years, and discusses the challenges faced and future development. This paper provides a comprehensive overview for researchers in the development of flexible wearable sensors for wound detection.

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Uricase Based Enzymatic Biosensor for Non‐invasive Detection of Uric Acid by Entrapment in PVA‐SbQ Polymer Matrix

Cited by 29 publications

References 67 publications

Designing Porous Antifouling Interfaces for High‐Power Implantable Biofuel Cell

Designing Porous Antifouling Interfaces for High‐Power Implantable Biofuel Cell

The effect of uricase on monosodium urate (MSU) crystallization, IL-1β and TNFα in monosodium urate-induced arthritis

A Review of Recent Advances in Flexible Wearable Sensors for Wound Detection Based on Optical and Electrical Sensing

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