Uric acid enzyme biosensor based on a screen-printed electrode coated with Prussian blue and modified with chitosan-graphene composite cryogel

Jirakunakorn, Ratchaneekorn; Khumngern, Suntisak; Choosang, Jittima; Thavarungkul, Panote; Kanatharana, Proespichaya; Numnuam, Apon

doi:10.1016/j.microc.2020.104624

Cited by 57 publications

(19 citation statements)

References 28 publications

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“…The value of Km is about 0.07 mM which is much lower than the corresponding values (0.17 to 7.83 mM) reported by other workers for uric acid biosensors [27]. Jirakunakorn et al [28] in his recent work on screen printed electrode prepared using Prussian blue and a cryogel prepared from the composite of chitosan and graphene has reported a uric acid biosensor which exhibits a Km of 0.23 mM. Also, the linear range is limited to 0.4 mM [28].…”

Section: Uric Acidmentioning

confidence: 54%

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Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Jindal

Gupta

Tomar

2021

Springer Proceedings in Materials

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A novel uric acid biosensor employing ZnO thin film as matrix is developed using pulsed laser deposition technique. The dependence of electrocatalytic properties of ZnO thin films on the ZnO processing pressure during growth is studied. It has been observed that the growth kinetics of ZnO matrix play a critical role in governing the electron transfer characteristics of ZnO thin film based biosensors. It is found from the cyclic voltammetric measurements that the peak oxidation current of ZnO/ITO/glass electrode increases with a rise in pressure of ambient gas from 1 to 100 mT and is maximum (548 µA) for ZnO thin film based electrode prepared in an oxygen ambient of 100 mT. The variation in peak current with change in processing pressure is attributed to the change in surface properties, which largely depends on the mean free path and kinetic energy of ablated species arriving at the substrate. The optimized ZnO thin film (100 mT) offers high surface coverage (9.74 × 10 -9 mol/cm 2 ) during immobilization of uric acid resulting in a sensitivity of 122 µA/(mM-cm 2 ). In addition, the prepared ZnO based biosensor exhibit high affinity towards detection of uric acid (Km~0.07 mM), low limit of detection (0.01 mM) along a storage stability of more than 20 weeks. Thus, the present work suggests an important role of plume kinetics for the fabrication of ZnO thin film based biosensors.

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Section: Uric Acidmentioning

confidence: 54%

“…Jirakunakorn et al [28] in his recent work on screen printed electrode prepared using Prussian blue and a cryogel prepared from the composite of chitosan and graphene has reported a uric acid biosensor which exhibits a Km of 0.23 mM. Also, the linear range is limited to 0.4 mM [28]. In another report, Numnuam et al…”

Section: Uric Acidmentioning

confidence: 98%

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Jindal

Gupta

Tomar

2021

Springer Proceedings in Materials

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“…Thus, NH 2 -VMSF/ErGO/SPCE sensor exhibits higher sensitivity and lower LOD, which is mainly due to the further enrichment of nanochannels. The LOD obtained on NH 2 -VMSF/ErGO/SPCE was lower than that obtained on Nafion-modified SPCE [ 33 ], nanodiamond modified SPE [ 34 ], Uricase/graphene-incorporated chitosan/Prussian blue modified SPCE (Uricase/Chi-Gr/PB/SPCE) [ 35 ], uricase-inorganic hybrid nanoflowers-Au nanoparticles modified SPCE (UOx-NFs-AuNPs-SPCE) [ 36 ], and Co 3 O 4 -ErGO modified SPE (Co 3 O 4 -ErGO/SPE) [ 37 ]. The low LOD of the developed sensor lies in the dual amplification effects resulting from ErGO and nanochannels.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Sensor Nanoarchitectonics for Sensitive Detection of Uric Acid in Human Whole Blood Based on Screen-Printed Carbon Electrode Equipped with Vertically-Ordered Mesoporous Silica-Nanochannel Film

Yang

Liu

et al. 2022

Nanomaterials

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Screen-printed carbon electrodes (SPCEs) bear great potential in the detection of biomarker in clinical samples with low sample consumption. However, modification of electrode surfaces to improve the anti-interference ability and sensitivity is highly desirable for direct electroanalysis of whole blood samples. Here, a reliable and miniaturized electrochemical sensor is demonstrated based on SPCE equipped with vertically-ordered mesoporous silica-nanochannel film (VMSF). To achieve stable binding of VMSF and improve the electrocatalytic performance, electrochemically reduced graphene oxide (ErGO) is applied as a conductive adhesion layer, that is in situ reduced from GO nanosheets during fast growth (less than 10 s) of amino groups modified VMSF (NH2-VMSF) using electrochemically assisted self-assembly (EASA). In comparison with bare SPCE, NH2-VMSF/ErGO/SPCE exhibits decreased oxidation potential of uric acid (UA) by 147 mV owing to significant electrocatalytic ability of ErGO. The dual signal amplification based on electrocatalysis of ErGO and enrichment of nanochannels leads to enhanced peak current by 3.9 times. Thus, the developed NH2-VMSF/ErGO/SPCE sensor enables sensitive detection of UA in the range from 0.5 μM to 180 μM with a low limit of detection (LOD, 129 nM, S/N = 3). Owing to good anti-fouling ability and high selectivity of the sensor, direct and rapid detection of UA in human whole blood is realized with very low sample consumption (50 μL).

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“…Prussian blue (PB) is part of an important group of inorganic compounds used in electrode modification and for electrocatalytic purposes [10,11]. PB is known as an artificial peroxidase [12,13], and it is a promising candidate for the catalysis of H 2 O 2 . The use of PB as a recognition element for H 2 O 2 detection is extensive [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on Prussian Blue Electrochemically Deposited at ZrO2 Doped Carbon Nanotubes Glassy Carbon Modified Electrode

et al. 2020

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In this work, a new hydrogen peroxide (H2O2) electrochemical sensor was fabricated. Prussian blue (PB) was electrodeposited on a glassy carbon (GC) electrode modified with zirconia doped functionalized carbon nanotubes (ZrO2-fCNTs), (PB/ZrO2-fCNTs/GC). The morphology and structure of the nanostructured system were characterized by scanning and transmission electron microscopy (TEM), atomic force microscopy (AFM), specific surface area, X-ray diffraction (XRD), thermogravimetric analysis (TGA), Raman and Fourier transform infrared (FTIR) spectroscopy. The electrochemical properties were studied by cyclic voltammetry (CV) and chronoamperometry (CA). Zirconia nanocrystallites (6.6 ± 1.8 nm) with cubic crystal structure were directly synthesized on the fCNTs walls, obtaining a well dispersed distribution with a high surface area. The experimental results indicate that the ZrO2-fCNTs nanostructured system exhibits good electrochemical properties and could be tunable by enhancing the modification conditions and method of synthesis. The fabricated sensor could be used to efficiently detect H2O2, presenting a good linear relationship between the H2O2 concentration and the peak current, with quantification limit (LQ) of the 10.91 μmol·L−1 and detection limit (LD) of 3.5913 μmol·L−1.

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Uric acid enzyme biosensor based on a screen-printed electrode coated with Prussian blue and modified with chitosan-graphene composite cryogel

Cited by 57 publications

References 28 publications

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Electrochemical Sensor Nanoarchitectonics for Sensitive Detection of Uric Acid in Human Whole Blood Based on Screen-Printed Carbon Electrode Equipped with Vertically-Ordered Mesoporous Silica-Nanochannel Film

Electrochemical Sensor Based on Prussian Blue Electrochemically Deposited at ZrO2 Doped Carbon Nanotubes Glassy Carbon Modified Electrode

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