Uric acid deposits and estivation in the invasive apple-snail, Pomacea canaliculata

Giraud-Billoud, Maximiliano; Abud, María; Cueto, Juan Agustín; Vega, Israel A.; Castro-Vázquez, Alfredo

doi:10.1016/j.cbpa.2010.12.012

Cited by 48 publications

(49 citation statements)

References 37 publications

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“…In contrast to P. maculata, the flexible edge of the operculum in P. canaliculata enables it to seal the shell hermetically, but is not as effective for this purpose in larger P. maculata with much thicker operculae (Bachman, 1960;Yoshida et al, 2014). However, even in cases in which it does form a substantial seal, some water is still lost during aestivation (Little, 1968;Giraud-Billoud et al, 2011). In P. canaliculata, the operculum of hatchlings and juveniles of both sexes is concave (Estebenet et al, 2006;Gamarra-Luques et al, 2013).…”

Section: Operculummentioning

confidence: 96%

“…Although some species switch to anaerobic respiration at some stage during dry-down events, the capacity for aerial gas exchange is particularly significant during such events, when the snails burrow in the mud (Meenakshi, 1956(Meenakshi, , 1964Burky et al, 1972;Thomas & Agard, 1992). The behavior displayed during experimental induction of aestivation and arousal from it are similar in P. lineata and P. canaliculata, but the process of entering aestivation takes 2-4 times longer in P. lineata (Little, 1968;Giraud-Billoud et al, 2011). Only in P. urceus is this dry dormancy thought to be an obligatory part of the life cycle, as drying conditions are a cue for the onset of reproduction (Ramnarine, 2003; see Life History).…”

Section: Aestivationmentioning

confidence: 96%

“…Mortality during aestivation in Pomacea species may be affected by duration, water conditions and body size (Little, 1968;Burky et al, 1972;Fan et al, 2000;Darby et al, 2003;Giraud-Billoud et al, 2011). Species differences seem particularly evident in mortality as a function of aestivation duration, but detailed comparisons are difficult, because most reports are of extreme survival times rather than changes in mortality rate over time (Cowie, 2002;Giraud-Billoud et al, 2011).…”

Section: Aestivationmentioning

confidence: 96%

See 2 more Smart Citations

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

et al. 2015

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Section: Operculummentioning

confidence: 96%

Section: Aestivationmentioning

confidence: 96%

Section: Aestivationmentioning

confidence: 96%

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Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

et al. 2015

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“…The role of antioxidant defences during water shortage has received special attention in terrestrial gastropods (Hermes-Lima et al, 1998;Hermes-Lima and Zenteno-Savin, 2002;RamosVasconcelos et al, 2005;Nowakowska et al, 2009;Nowakowska et al, 2010;Nowakowska et al, 2011) and has also been studied in some aquatic gastropods (Ferreira et al, 2003;Giraud-Billoud et al, 2011). In general, terrestrial gastropods have stable levels of antioxidant defences such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) (Hermes-Lima and Storey, 1995;Nowakowska et al, 2009;Nowakowska et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and molecular chaperone defenses during estivation and arousal in the South American apple-snailPomacea canaliculata

Giraud-Billoud

Vega

Tosi

et al. 2012

Journal of Experimental Biology

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SUMMARYThe invasive Pomacea canaliculata estivates during periods of drought and should cope with harmful effects of reoxygenation during arousal. We studied thiobarbituric acid reactive substances (TBARS), enzymatic (superoxide dismutase, SOD and catalase, CAT) and non-enzymatic antioxidants (uric acid and reduced glutathione), and heat shock protein expression (Hsc70, Hsp70 and Hsp90) in (1) active control snails, (2) snails after 45days of estivation, and (3) aroused snails 20min and (4) 24h after water exposure, in midgut gland, kidney and foot. Both kidney and foot (but not the midgut gland) showed a TBARS increase during estivation and a decrease after arousal. Tissue SOD and CAT did not change in any experimental groups. Uric acid increased during estivation in all tissues, and it decreased after arousal in the kidney. Allantoin, the oxidation product of uric acid, remained constant in the midgut gland but it decreased in the kidney until 20min after arousal; however, allantoin levels rose in both kidney and foot 24h after arousal. Reduced glutathione decreased during estivation and arousal, in both midgut gland and kidney, and it remained constant in the foot. Hsc70 and Hsp70 kidney levels were stable during the activity-estivation cycle and Hsp90 expression decreases during estivation and recovers in the early arousal. In foot, the expression of Hsp70 and Hsp90 was high during activity and estivation periods and disminished after arousal. Results indicate that a panoply of antioxidant and molecular chaperone defences may be involved during the activity-estivation cycle in this freshwater gastropod.

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“…The hepatopancreas was selected for analysis as this organ is important for energy metabolism and waste storage, and its protein expression has been shown to be responsive to another environmental stressor (desiccation). [44][45][46] Protein extraction, digestion and labelling Samples were placed on ice to reduce the influence of heat. Hepatopancreas tissues were homogenized using a plastic pestle.…”

Section: Protein Expressionmentioning

confidence: 99%

Genetic Basis of Differential Heat Resistance between Two Species of Congeneric Freshwater Snails: Insights from Quantitative Proteomics and Base Substitution Rate Analysis

Sun

Fang

et al. 2015

J. Proteome Res.

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We compared the heat tolerance, proteomic responses to heat stress, and adaptive sequence divergence in the invasive snail Pomacea canaliculata and its noninvasive congener Pomacea diffusa. The LT50 of P. canaliculata was significantly higher than that of P. diffusa. More than 3350 proteins were identified from the hepatopancreas of the snails exposed to acute and chronic thermal stress using iTRAQ-coupled mass spectrometry. Acute exposure (3 h exposure at 37 °C with 25 °C as control) resulted in similar numbers (27 in P. canaliculata and 23 in P. diffusa) of differentially expressed proteins in the two species. Chronic exposure (3 weeks of exposure at 35 °C with 25 °C as control) caused differential expression of more proteins (58 in P. canaliculata and 118 in P. diffusa), with many of them related to restoration of damaged molecules, ubiquitinating dysfunctional molecules, and utilization of energy reserves in both species; but only in P. diffusa was there a shift from carbohydrate to lipid catabolism. Analysis of orthologous genes encoding the differentially expressed proteins revealed two genes having clear evidence of positive selection (Ka/Ks > 1) and seven candidates for more detailed analysis of positive selection (Ka/Ks between 0.5 and 1). These nine genes are related to energy metabolism, cellular oxidative homeostasis, signaling, and binding processes. Overall, the proteomic and base substitution rate analyses indicate genetic basis of differential resistance to heat stress between the two species, and such differences could affect their further range expansion in a warming climate.

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Uric acid deposits and estivation in the invasive apple-snail, Pomacea canaliculata

Cited by 48 publications

References 37 publications

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

Antioxidant and molecular chaperone defenses during estivation and arousal in the South American apple-snailPomacea canaliculata

Genetic Basis of Differential Heat Resistance between Two Species of Congeneric Freshwater Snails: Insights from Quantitative Proteomics and Base Substitution Rate Analysis

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