Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes

Huh, Yeowool; Cho, Jeiwon

doi:10.3389/fnbeh.2013.00141

Cited by 20 publications

(17 citation statements)

References 19 publications

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“…The effects described here might have been modulated by urethane anesthesia that influences excitatory and inhibitory neuronal firing (Hara and Harris, 2002) and, consequently, might differ from multisensory interactions in the awake state (Iurilli et al, 2012; Ibrahim et al, 2016). Furthermore, it has been shown that urethane anesthesia strengthens thalamic burst firing which, in contrast to tonic firing mode, inhibits the transmission of sensory information to the cortex (Huh and Cho, 2013). By these means, the flow of unisensory and multisensory information to primary sensory cortex might be restricted under urethane anesthesia.…”

Section: Discussionmentioning

confidence: 99%

Rate and Temporal Coding Convey Multisensory Information in Primary Sensory Cortices

Bieler

Sieben

Cichon

et al. 2017

eNeuro

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Optimal behavior and survival result from integration of information across sensory systems. Modulation of network activity at the level of primary sensory cortices has been identified as a mechanism of cross-modal integration, yet its cellular substrate is still poorly understood. Here, we uncover the mechanisms by which individual neurons in primary somatosensory (S1) and visual (V1) cortices encode visual-tactile stimuli. For this, simultaneous extracellular recordings were performed from all layers of the S1 barrel field and V1 in Brown Norway rats in vivo and units were clustered and assigned to pyramidal neurons (PYRs) and interneurons (INs). We show that visual-tactile stimulation modulates the firing rate of a relatively low fraction of neurons throughout all cortical layers. Generally, it augments the firing of INs and decreases the activity of PYRs. Moreover, bimodal stimulation shapes the timing of neuronal firing by strengthening the phase-coupling between neuronal discharge and theta–beta band network oscillations as well as by modulating spiking onset. Sparse direct axonal projections between neurons in S1 and V1 seem to time the spike trains between the two cortical areas and, thus, may act as a substrate of cross-modal modulation. These results indicate that few cortical neurons mediate multisensory effects in primary sensory areas by directly encoding cross-modal information by their rate and timing of firing.

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Section: Discussionmentioning

confidence: 99%

Rate and Temporal Coding Convey Multisensory Information in Primary Sensory Cortices

Bieler

Sieben

Cichon

et al. 2017

eNeuro

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“…Urethane1.5 induced deeper anaesthesia than observed in the other groups, but yielded high ReHo, a seemingly contradictory finding that may be partly explained by the fact that urethane exerts modest effects on multiple receptor systems. However, one study in mice using the similar level of urethane reported depressed thalamic activity, indicating that there may also be other reasons for the retained ReHo under Urethane1.5 (Huh and Cho, 2013).…”

Section: Discussionmentioning

confidence: 99%

Altered regional connectivity reflecting effects of different anaesthesia protocols in the mouse brain

Grandjean

Bosshard

et al. 2017

NeuroImage

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Altered regional connectivity reflecting effects of different anaesthesia protocols in the mouse brain, NeuroImage, http://dx.doi.org/10.1016/j.neuroimage. 2017.01.074 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractStudies in mice using resting-state functional magnetic resonance imaging (rs-fMRI) have provided opportunities to investigate the effects of pharmacological manipulations on brain function and map the phenotypes of mouse models of human brain disorders. Mouse rs-fMRI is typically performed under anaesthesia, which induces both regional suppression of brain activity and disruption of large-scale neural networks. Previous comparative studies using rodents investigating various drug effects on long-distance functional connectivity (FC) have reported agent-specific FC patterns, however, effects of regional suppression are sparsely explored. Here we examined changes in regional connectivity under six different anaesthesia conditions using mouse rs-fMRI with the goal of refining the framework of understanding the brain activation under anaesthesia at a local level. Regional homogeneity (ReHo) was used to map local synchronization in the brain, followed by analysis of several brain areas based on ReHo maps. The results revealed high local coherence in most brain areas. The primary somatosensory cortex and caudate-putamen showed agent-specific properties. Lower local coherence in the cingulate cortex was observed under medetomidine, particularly when compared to the combination of medetomidine and isoflurane. The thalamus was associated with retained local coherence across anaesthetic levels and multiple nuclei. These results show that anaesthesia induced by the investigated anaesthetics through different molecular targets promote agent-specific regional connectivity. In addition, ReHo is a data-driven method with minimum user interaction, easy to use and fast to compute. Given that examination of the brain at a local level is widely applied in human rs-fMRI studies, our results show its sensitivity to extract information on varied neuronal activity under six different regimens relevant to mouse functional imaging. These results, therefore, will inform future rsfMRI studies on mice and the type of anaesthetic agent used, and will help to bridge observations between this burgeoning research field and ongoing human research across analytical scales.

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“…CMG was conducted under urethane anesthetic, which preserves reflex voiding to continuous infusion with minimal impact on cystometric parameters . Urethane inhibits suprathalamic sensory transmission thereby suppressing cortical/social control over voiding, thus CMG under urethane anesthesia tests autonomic reflex bladder emptying in response to the physiologic challenge of continuous bladder filling …”

Section: Methodsmentioning

confidence: 99%

The aging bladder phenotype is not the direct consequence of bladder aging

Hardy

Keilich

Harrison

et al. 2019

Neurourology and Urodynamics

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Aims The prevalence of urinary dysfunction increases with age, yet therapies are often suboptimal. Incomplete understanding of the linkages between system, organ, and tissue domains across lifespan remains a knowledge gap. If tissue‐level changes drive the aging bladder phenotype, parallel changes should be observed across these domains. In contrast, a lack of inter‐domain correlation across age groups would support the hypothesis that urinary performance is a measure of the physiologic reserve, dependent on centrally‐mediated adaptive mechanisms in the aging system. Methods Male and female mice across four age groups underwent sequential voiding spot assays, pressure/flow cystometry, bladder strip tension studies, histology, and quantitative PCR analyses. The primary objective of this study was to test the impact of age on the cortical, autonomic, tissue functional and structural, and molecular domains, and identify inter‐domain correlations among variables showing significant changes with age within these domains. Results Behavior revealed diminished peripheral voiding and spot size in aged females. Cystometry demonstrated increased postvoid residual and loss of volume sensitivity, but the preservation of voiding contraction power, with almost half of oldest‐old mice failing under cystometric stress. Strip studies revealed no significant differences in adrenergic, cholinergic, or EFS sensitivity. Histology showed increased detrusor and lamina propria thickness, without a change in collagen/muscle ratio. Adrb2 gene expression decreased with age. No consistent inter‐domain correlations were found across age groups. Conclusions Our findings are consistent with a model in which centrally‐mediated adaptive failures to aging stressors are more influential over the aging bladder phenotype than local tissue changes.

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Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes

Cited by 20 publications

References 19 publications

Rate and Temporal Coding Convey Multisensory Information in Primary Sensory Cortices

Rate and Temporal Coding Convey Multisensory Information in Primary Sensory Cortices

Altered regional connectivity reflecting effects of different anaesthesia protocols in the mouse brain

The aging bladder phenotype is not the direct consequence of bladder aging

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