Ureasil-Based Polymer Matrices As Sensitive Layers for the Construction of Amperometric Biosensors

Controlling the Network Properties of Polymer Matrices for Improvement of Amperometric Enzyme Biosensors: Contribution of Positron Annihilation

“…Two types of polymers such as an organic-inorganic hybrid ureasilicate (shortened name -ureasil) and a photocross-linked polymer were selected for research. The synthesis of the ureasil-based polymers (pure ureasil and ureasil/As 2 S 3 composite) was reported in [1][2][3][4].…”

“…Biosensitive layers of the amperometric biosensor were formed on the surface of solid state transducers. In case of the ureasil-based polymers, planar gold electrodes DRP-C220AT from "DropSens" (Llanera, Asturias, Spain) were used as working electrodes [1,3]. In case of the photocross-linked polymers, graphite rods (type RW001, 3.05 mm diameter, area 7.3 mm 2 , Ringsdorff Werke, Bonn, Germany) were used as working electrodes [5].…”

“…In case of the photocross-linked polymers, graphite rods (type RW001, 3.05 mm diameter, area 7.3 mm 2 , Ringsdorff Werke, Bonn, Germany) were used as working electrodes [5]. Immobilization of laccase was described for the ureasil-based polymers in [1,3] and for the photocross-linked polymers in [5].…”

“…The estimation of the optimal working potential with cyclic voltamperometry and the conduction of an analysis with a chronoamperometric approach with respect to the corresponding substrate (2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid, ABTS) were performed for the constructed laccase-based amperometric enzyme biosensors of the third generation for analysis of phenol derivates using ureasil-based polymers [1,3] and photocross-linked polymers [5].…”

“…At the same time, positron annihilation lifetime spectroscopy (PALS) is known as a progressive method for microstructural analysis of macromolecular structures. Combination of both methods allowed to get information about network properties of the polymer matrices and the results obtained will be further compared with sensitivity of bioelectrodes constructed using the polymer matrices [1][2][3][4][5]. A role of free-volume and crosslink density in the host polymer matrices used for improvement of operational parameters of laccase-based amperometric biosensors would be established.…”