Urease from leaves of Glycine max and Zea mays

Davies, H.Maelor; Shih, Liu-Mei

doi:10.1016/0031-9422(84)83007-1

Cited by 15 publications

(12 citation statements)

References 11 publications

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“…5A) was determined using leaf extracts. This is in close agreement with the K m value of 0.5 mM from ureases of G. max and Zea mays (Davies and Shih, 1984).…”

Section: Biochemical Characterization Of Rice Urease and Arginasesupporting

confidence: 78%

Identification and Characterization of Proteins Involved in Rice Urea and Arginine Catabolism

Cao

Werner²,

Dahncke³

et al. 2010

Plant Physiology

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Rice (Oryza sativa) production relies strongly on nitrogen (N) fertilization with urea, but the proteins involved in rice urea metabolism have not yet been characterized. Coding sequences for rice arginase, urease, and the urease accessory proteins D (UreD), F (UreF), and G (UreG) involved in urease activation were identified and cloned. The functionality of urease and the urease accessory proteins was demonstrated by complementing corresponding Arabidopsis (Arabidopsis thaliana) mutants and by multiple transient coexpression of the rice proteins in Nicotiana benthamiana. Secondary structure models of rice (plant) UreD and UreF proteins revealed a possible functional conservation to bacterial orthologs, especially for UreF. Using aminoterminally StrepII-tagged urease accessory proteins, an interaction between rice UreD and urease could be shown. Prokaryotic and eukaryotic urease activation complexes seem conserved despite limited protein sequence conservation for UreF and UreD. In plant metabolism, urea is generated by the arginase reaction. Rice arginase was transiently expressed as a carboxylterminally StrepII-tagged fusion protein in N. benthamiana, purified, and biochemically characterized (K m = 67 mM, k cat = 490 s 21 ). The activity depended on the presence of manganese (K d = 1.3 mM). In physiological experiments, urease and arginase activities were not influenced by the external N source, but sole urea nutrition imbalanced the plant amino acid profile, leading to the accumulation of asparagine and glutamine in the roots. Our data indicate that reduced plant performance with urea as N source is not a direct result of insufficient urea metabolism but may in part be caused by an imbalance of N distribution.

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“…5A) was determined using leaf extracts. This is in close agreement with the K m value of 0.5 mM from ureases of G. max and Zea mays (Davies and Shih, 1984).…”

Section: Biochemical Characterization Of Rice Urease and Arginasesupporting

confidence: 78%

Identification and Characterization of Proteins Involved in Rice Urea and Arginine Catabolism

Cao

Werner²,

Dahncke³

et al. 2010

Plant Physiology

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“…The acetate buffer is used to equilibrate the gel at pH 6.0 to avoid unspecific dye precipitation during staining, but this pH coincides with a minimum in leaf urease activity. Maximum urease activity was observed at pH 5.5 and a second broad maximum occurs at pH 7.0 -8.8 as established in several plants (6,14). The localized production of NH 4 ϩ raises the pH in the vicinity of urease bands.…”

Section: Discussionmentioning

confidence: 96%

In-Gel Detection of Urease with Nitroblue Tetrazolium and Quantification of the Enzyme from Different Crop Plants Using the Indophenol Reaction

Witte¹,

Medina‐Escobar²

2001

Analytical Biochemistry

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“…Urease extraction from plant material usually involves grinding fresh plant material immediately or flash freezing the tissue with liquid nitrogen before processing. Davies and Shih (1984) reported greater urease activities in urease extracts from fresh soybean leaves compared to corn leaves. This difference could be due to greater activity in soybeans or more urease being produced by soybean than corn.…”

Section: Introductionmentioning

confidence: 93%

“…This is due to the inhibitory effect of the orthophosphate ion (H 2 PO 4 − ), which deprotonates as the pH approaches 7.2 and upon deprotonation decreases the inhibition of urease (Krajewska and Zaborska 1999). Krajewska (2009) reported pH optima for soybean urease activity to be 7.0 and jackbean urease activity at pH 7.0-7.5, whereas Davies and Shih (1984) reported activity optima for soybean and corn leaf urease to be at 5.5 and 8.8 for soybean and 5.0-5.5, 7.5, and 8.8 for corn.…”

Section: Introductionmentioning

confidence: 93%

“…The use of buffered solutions containing sodium or potassium phosphate, ethylenediaminetetraacetic acid (EDTA), sodium chloride, and 2-mercaptoethanol are the most common chemical agents utilized in urease extracting solutions (Davies and Shih 1984;Witte and Medina-Escobar 2001;Follmer et al 2004). Phosphate buffer solutions have been shown to be competitive inhibitors of urease as the concentration of phosphate increases in the buffering solutions and as pH decreases.…”

Section: Introductionmentioning

confidence: 99%

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Ureolytic Activity of Soybean and Corn Residue Extracts

Frame

Ridgley

Gaasch

et al. 2014

Communications in Soil Science and Plant Analysis

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The enzyme urease is responsible for the rapid hydrolysis of urea in agroecosystems where more than 50% of the applied nitrogen (N) can be lost via ammonia volatilization. The objectives of the study were to (1) extract urease from corn (Zea mays L.) and soybean (Glycine max L.) and (2) compare the urease activity from soybean and corn residues to Jackbean (Canavalia ensiformis) urease using Fourier transform infrared spectroscopy (FTI-R). Concentrations of urea and sodium bicarbonate ranging from 0 to 200 mM were analyzed with FTI-R to determine the correlation to peak height. Bicarbonate produced the most responsive peaks to concentration at 1,362 cm −1 . Urease extracted from soybean residue was active, producing a bicarbonate peak at 1362 cm −1 , whereas no urease activity was observed in corn residue. Variation among urease activities in crop residues could suggest a need for more precise nitrogen management in conservation tillage agroecosystems to reduce ammonia volatilization.

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Urease from leaves of Glycine max and Zea mays

Cited by 15 publications

References 11 publications

Identification and Characterization of Proteins Involved in Rice Urea and Arginine Catabolism

Identification and Characterization of Proteins Involved in Rice Urea and Arginine Catabolism

In-Gel Detection of Urease with Nitroblue Tetrazolium and Quantification of the Enzyme from Different Crop Plants Using the Indophenol Reaction

Ureolytic Activity of Soybean and Corn Residue Extracts

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