Urease-based ISFET biosensor for arginine determination

Sheliakina, Margaryta; Arkhypova, V. М.; Солдаткін, О. О.; Saiapina, Olga; Akata, Burcu; Dzyadevych, S. V.

doi:10.1016/j.talanta.2013.12.042

Cited by 38 publications

(19 citation statements)

References 23 publications

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“…One millimolar l-Arg solutions in the pH range of 5.2-11.0 were exposed to the l-Arg-sensing membrane, whereas the l-Asn-sensing membrane was tested with 0.4 mM l-Asn in the pH range of 5.2-9.3. The l-Arg-sensing membranes were tested at different temperatures (25,30,33,37, and 40°C) at 1, 5, and 10 mM l-Arg, and the l-Asn-sensing membrane was tested at 21, 28, 31, 33, 36, and 40°C at 5 mM l-Asn.…”

Section: Measurements Of L-arg and L-asnmentioning

confidence: 99%

Ratiometric fluorescent l‐arginine and l‐asparagine biosensors based on the oxazine 170 perchlorate‐ethyl cellulose membrane

Duong

Rhee

2017

Engineering in Life Sciences

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Ratiometric fluorescent l-arginine (Arg) and l-asparagine (Asn) biosensors were developed using an oxazine 170 perchlorate (O17) ethyl cellulose (EC) membrane and the enzymes entrapped into the matrix of EC and hydrogel polyurethane. The sensing principles were based on the hydrolysis reactions of urea and l-Arg under the catalysis of the urease and arginase to produce ammonia in the case of an l-Argsensing membrane and also on the hydrolysis reaction of l-Asn under the catalysis of asparaginase in the case of an l-Asn-sensing membrane. The O17-EC membrane reacted with the ammonia produced from the hydrolysis reactions and changed the fluorescence intensities at λ em = 565 and 625 nm. The ratio of the fluorescence intensities at λ em = 565 and 625 nm was proportional to the concentrations of l-Arg or l-Asn in the range of 0.1-10 mM. The LOD of the l-Arg-and l-Asn-sensing membranes was 0.082 ± 0.0014 and 0.074 ± 0.0023 mM, respectively. The sensing membranes also showed good quality in terms of response time, reversibility, and stability. The interference study demonstrated that some components such as amino acids had little negative effects on the performance of the sensing membranes for the detection of l-Arg and l-Asn. These simple and sensitive ratiometric fluorescent sensing membranes provide a basic or comprehensive method for detecting l-Arg and l-Asn in blood and urine samples as well as in the fermentation processes.Keywords: l-Arginine / l-Asparagine / O17-EC membrane / Ratiometric fluorescent biosensor Additional supporting information may be found in the online version of this article at the publisher's web-site

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Section: Measurements Of L-arg and L-asnmentioning

confidence: 99%

Ratiometric fluorescent l‐arginine and l‐asparagine biosensors based on the oxazine 170 perchlorate‐ethyl cellulose membrane

Duong

Rhee

2017

Engineering in Life Sciences

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“…When working, arginine catalyzes the conversion of arginine into ornithine with release of urea, which, in turn, is degraded by urease to produce ammonium ions. Production of ammonium is accompanied by the subtle change of pH and thus can be detected by the transmitter [30].…”

Section: Amino Acid Detectionmentioning

confidence: 99%

Biosensors in Fermentation Applications

Shi¹,

Feng²,

Li³

2017

Fermentation Processes

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Biosensing technology offers new analytic routes to the use and study of fermentations, taking advantage of the high selectivity and sensitivity of the bioactive elements it exploits. Various biosensors had been commercially available today; they provide fermentation processes with convenient, accurate, and cost-effective ways of monitoring for key biochemical parameters. In this chapter, the basic ideas and principles of biosensors, especially applications of the most popular biosensors related to fermentations were highlighted.

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“…Potentiometric biosensor was based on pH-sensitive field effect transistors as current transducers (Sheliakina et al, 2014;Pavluchenko et al, 2011). Each transducer contained a differential pair of pHsensitive field effect transistors placed on a single crystal with the total area of 8 mm × 8 mm.…”

Section: Potentiometric Urea Biosensormentioning

confidence: 99%

Development of Multi-parameter Analyser based on Electrochemical Urea Biosensors and Electrolyte Electrodes for Monitoring of Hemodialysis Patients

Razumienė

Gureviciene

Dagys

et al. 2016

Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies

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The idea of developing multi-parameter urea analyser comprising urea, Na + and K + selective electrodes has been considered. For this purpose the urea biosensors based on urease and recombinant urease working in amperometric and potentiometric way were developed. The working parameters of both urea biosensors were studied and optimized. Possibilities of real samples analysis using the developed biosensors were shown by measuring urea concentrations in blood dialysate taken from patients with renal failure. Both the potentiometric and the amperometric biosensors demonstrated high degree of signal reproducibility (the relative standard deviation of responses did not exceed 5 %). Change of sodium and potassium concentrations during blood hemodialysis is dangerous life-threatening condition and their monitoring is an important feature of point-of-care analyser. For this purpose high integrity commercial Na + and K + selective electrodes were analysed and our own signal amplification and processing system proposed.

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Urease-based ISFET biosensor for arginine determination

Cited by 38 publications

References 23 publications

Ratiometric fluorescent l‐arginine and l‐asparagine biosensors based on the oxazine 170 perchlorate‐ethyl cellulose membrane

Ratiometric fluorescent l‐arginine and l‐asparagine biosensors based on the oxazine 170 perchlorate‐ethyl cellulose membrane

Biosensors in Fermentation Applications

Development of Multi-parameter Analyser based on Electrochemical Urea Biosensors and Electrolyte Electrodes for Monitoring of Hemodialysis Patients

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