Urease activity in the gastrointestinal tract of the European hare (Lepus europaeus)

Stepan’kov, A. A.; Кузнецова, Т. А.; Vecherskii, M. V.

doi:10.1134/s1062359017020194

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…Unlike other monogastric animals, urease activity in the GI tract in rabbits would provide distinct advantages because of the synthesis of microbial protein in the large intestine and its reutilization for their coprophagy habits. In European hares, urease activity was detected to some extent in the stomach arising probably from cecotrophs, followed by no urease activity in the duodenum, and again detectable urease activity in the jejunum [40] . Expectedly, the large intestine (cecum and colon) contained highest urease activity with peak values of 4.2 mg ammonia-N/g/h) in the cecum [40] .…”

Section: Urease Enzymementioning

confidence: 94%

“…In European hares, urease activity was detected to some extent in the stomach arising probably from cecotrophs, followed by no urease activity in the duodenum, and again detectable urease activity in the jejunum [40] . Expectedly, the large intestine (cecum and colon) contained highest urease activity with peak values of 4.2 mg ammonia-N/g/h) in the cecum [40] . Several studies showed that strong urease activity is present in the cecum of the rabbits [41] , [42] .…”

Section: Urease Enzymementioning

confidence: 94%

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Ureases in the gastrointestinal tracts of ruminant and monogastric animals and their implication in urea-N/ammonia metabolism: A review

Patra

Aschenbach

2018

Journal of Advanced Research

109

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Section: Urease Enzymementioning

confidence: 94%

Section: Urease Enzymementioning

confidence: 94%

Ureases in the gastrointestinal tracts of ruminant and monogastric animals and their implication in urea-N/ammonia metabolism: A review

Patra

Aschenbach

2018

Journal of Advanced Research

109

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“…In animals, it is being used as convenient quantitative measure of nitrogen recycling. Particularly in European hare (Lepus europaeus), urease activity has been found to be high in winter periods for proper recycling of nitrogen from diet [72]. In silkworm, urease is important for the nitrogen metabolism of silkworms because the formation of ammonia is assimilated into silk protein.…”

Section: Discussionmentioning

confidence: 99%

Detection of gut microflora and enzymes using metagenomics and metaproteomics in silkworm, Bombyx mori

Mohanraj

Mahalingam

Karthikeyan³

et al. 2023

Preprint

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Background Microorganisms living in insect gut plays a crucial role in the adaptation, growth and development of the insect hosts. The identification and molecular analysis of insect gut microbes will enable us to develop novel strategies for industrial product development, effective utilization of by products and facilitate us with the best pest management practices. In our present study, we employed a culture independent metagenomic approach in conjunction with metaproteomic profiling to enumerate the gut microbes in pure races and cross breeds of silkworm, Bombyx mori L.Results The phylogenetic analysis showed that the gut microflora was majorly grouped into four bacterial classes: Gamma Proteobacteria, Actinobacteria, Cocci and Bacilli. Two novel proteins in both silkworm races were identified and an additional three proteins were found to be differentially expressed between the races.Conclusions Silkworm undergoes radical morphological variations upon metamorphosis and a key challenge in studying gut microflora is the ability to access different genomes and the corresponding protein profiles. The results presented in this study may help to bridge the gap between the unknowns of silkworm gut microbiota and their importance in the silkworm gut ecosystem.

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“…This resultant pH elevation is of diverse clinical and agronomic significance [3]. Although mammalian cell does not produce urease, it enters the mammalian body through different bacteria, especially within the intestine [4]. Urease is a virulence factor for Helicobacter pyroli, Proteus mirabilis, S. saprophyticus and Mycobacterium tuberculosis among others that are associated with certain diseases in human and animals.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of urease inhibitory and free radical scavenging activities of Microsorum pustulatum (G. Frost) copel leaves (Polypodiaceae)

Olebunne¹,

Afieroho²,

Suleiman³

et al. 2022

Int. J. Biol. Pharm. Sci. Arch.

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Microsorum pustulatum is an epiphytic fern. Young leaves and stems are edible, used in wound healing (ulcer), treatment of inflammation and skin infections. This study is reporting the effect of the leaves extracts and fractions of Microsorum pustulatum on the urease enzyme as well as their free radical scavenging activity. The leaves extracts were obtained by successive cold maceration in n-hexane, acetone and 70% aqueous ethanol. Phytochemical screening was done using standard methods. Urease inhibition assay was done using the modified Berthelot method with thiourea as reference standard urease inhibitor. The free radical scavenging activity was assayed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) with ascorbic acid as reference standard. The organic (AOP) and aqueous (AAP) fractions were obtained by chloroform-water partitioning of the most active acetone extract. The acetone extract (anti-urease=51.6%, DPPH radical scavenging= 49.9%) and its AOP fraction (anti-urease= 43.3%, DPPH radical scavenging= 70.31%) were observed to have good urease inhibition and free radical scavenging effect when compared with standard at the same 1mg/ml test concentration. The trend in median DPPH radical inhibition concentration: AOP (IC50 = 0.09 mg/ml) > AAP (IC50=0.64 mg/ml) was also observed. The phyto-constituent present in the screened leaf extract are saponins, tannins, phlobatannins, phenolic, flavonoids, terpenoids, steroids, carbohydrates. In conclusion the result validates the traditional use of Microsorum pustulatum leaves in treatment of disease such as ulcer, chronic inflammation etc. caused by pathogenic microorganism (Helicobacter pyroli, Proteus mirabilis etc.) that needs urease for survival and to curb inflammation in oxidative stress state. Also acetone extract of the plant can be used as additive to urea base fertilizer in the development of ecofriendly urease inhibitors and increase in crop production.

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Urease activity in the gastrointestinal tract of the European hare (Lepus europaeus)

Cited by 5 publications

References 21 publications

Ureases in the gastrointestinal tracts of ruminant and monogastric animals and their implication in urea-N/ammonia metabolism: A review

Ureases in the gastrointestinal tracts of ruminant and monogastric animals and their implication in urea-N/ammonia metabolism: A review

Detection of gut microflora and enzymes using metagenomics and metaproteomics in silkworm, Bombyx mori

Evaluation of urease inhibitory and free radical scavenging activities of Microsorum pustulatum (G. Frost) copel leaves (Polypodiaceae)

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