Urea-treated Gram-negative Bacilli in the Indirect Hæmagglutination Reaction

Brodhage, H

doi:10.1038/193501a0

Cited by 5 publications

(3 citation statements)

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“…It will be interesting to learn whether cytophilic antibodies with CA specificity do occur. CA may represent only one of several antigens shared by enteric bacteria; for example, Brodhage (2,3,4) demonstrated another common antigen in urea extracts of various Enterobacteriaceae.…”

Section: Discussionmentioning

confidence: 99%

Opsonizing and Bactericidal Activity of Antibodies Against Common Antigen of Enterobacteriaceae

Domingue

Neter

1966

J Bacteriol

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DOMINGUE, GERALD J. (State University of New York at Buffalo, Buffalo, N.Y.), AND ERWIN NETER. Opsonizing and bactericidal activity of antibodies against common antigen of Enterobacteriaceae. J. Bacteriol. 91:129-133. 1966.-In addition to the well-known 0, H, K, and Vi antigens, Enterobacteriaceae produce a common antigen, which was first identified by hemagglutination tests with Escherichia coli 014 antiserum. Studies on the biological significance of this antigen by in vitro phagocytic experiments have revealed that opsonization is markedly enhanced in the presence of the corresponding antibody, rabbit polymorphonuclear leukocytes, and normal rabbit serum. This effect is specific, because Pseudomonas aeruginosa, which is devoid of this antigen, is not opsonized under these conditions, and removal of the antibody by absorption markedly reduces the uptake of enteric bacteria containing the antigen. Opsonization thus represents another method for the study of this antigen-antibody system. Bactericidal tests have revealed that antibodies against this antigen, engendered in rabbits by different strains of enteric bacteria and various procedures, are bactericidal for E. coli 014 but not for other enteric bacteria, possibly due to previously demonstrated differences between the antigen moieties obtained from these microorganisms.

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Section: Discussionmentioning

confidence: 99%

Opsonizing and Bactericidal Activity of Antibodies Against Common Antigen of Enterobacteriaceae

Domingue

Neter

1966

J Bacteriol

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“…Hemagglutination (HA), Hemolysis, and Hemagglutination Inhibition (HAI) Fresh human (blood group 0) or sheep RBC have been used mostly, but probably other RBC and formalin-preserved cells work as well (22,139,216). These are coated by the sensitizing ECA preparations and thus become reactive with anti-ECA sera.…”

Section: Serological Methodsmentioning

confidence: 99%

“…LPS prepared by the hot phenol-water extraction method or by trichloroacetic acid extraction as the Boivin antigen (109) is also reactive (102), whereas LPS ex- tracted with the phenol-chloroform-petroleum ether method is not (126). Brodhage (20)(21)(22) used urea extracts ofbacteria in a similar assay and detected a common antigen-probably ECA, although the exact correlation has not been studied (see also "Other cross-reactivities and related antigens").…”

Section: Serological Methodsmentioning

confidence: 99%